Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3890

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntGFF3888 and GFF3889 are separated by 41 nucleotidesGFF3889 and GFF3890 are separated by 3 nucleotidesGFF3890 and GFF3891 are separated by 5 nucleotidesGFF3891 and GFF3892 overlap by 4 nucleotides GFF3888 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 167,358 to 168,257 GFF3888 GFF3889 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 168,299 to 169,183 GFF3889 GFF3890 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 169,187 to 169,735 GFF3890 GFF3891 - CDP-6-deoxy-delta-3,4-glucoseen reductase-like, at 169,741 to 170,733 GFF3891 GFF3892 - Glucose-1-phosphate cytidylyltransferase (EC 2.7.7.33), at 170,730 to 171,503 GFF3892 Position (kb) 169 170Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 168.264 kb on + strandat 169.309 kb on + strand, within GFF3890at 169.598 kb on + strand, within GFF3890at 169.648 kb on + strand, within GFF3890at 170.055 kb on - strand, within GFF3891at 170.087 kb on + strand, within GFF3891at 170.442 kb on + strand, within GFF3891at 170.606 kb on + strand, within GFF3891

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
remove
168,264 + +1.5
169,309 + GFF3890 0.22 -3.9
169,598 + GFF3890 0.75 -4.2
169,648 + GFF3890 0.84 -4.2
170,055 - GFF3891 0.32 -2.0
170,087 + GFF3891 0.35 -1.8
170,442 + GFF3891 0.71 -1.4
170,606 + GFF3891 0.87 -1.8

Or see this region's nucleotide sequence