Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3860 and GFF3861 are separated by 75 nucleotides GFF3861 and GFF3862 are separated by 90 nucleotides GFF3862 and GFF3863 are separated by 38 nucleotides
GFF3860 - FIG01045963: hypothetical protein, at 136,407 to 136,577
GFF3860
GFF3861 - Uridine kinase (EC 2.7.1.48) [C1], at 136,653 to 137,294
GFF3861
GFF3862 - Deoxycytidine triphosphate deaminase (EC 3.5.4.13), at 137,385 to 137,966
GFF3862
GFF3863 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 138,005 to 139,861
GFF3863
Position (kb)
137
138 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 136.388 kb on - strand at 136.388 kb on - strand at 136.393 kb on - strand at 136.393 kb on - strand at 136.396 kb on - strand at 136.430 kb on + strand, within GFF3860 at 136.586 kb on + strand at 136.623 kb on + strand at 136.623 kb on + strand at 136.623 kb on + strand at 136.624 kb on - strand at 136.797 kb on + strand, within GFF3861 at 136.800 kb on + strand, within GFF3861 at 136.801 kb on - strand, within GFF3861 at 136.848 kb on + strand, within GFF3861 at 136.849 kb on - strand, within GFF3861 at 136.935 kb on + strand, within GFF3861 at 136.935 kb on + strand, within GFF3861 at 136.936 kb on - strand, within GFF3861 at 136.941 kb on + strand, within GFF3861 at 136.942 kb on - strand, within GFF3861 at 136.997 kb on + strand, within GFF3861 at 137.009 kb on + strand, within GFF3861 at 137.209 kb on - strand, within GFF3861 at 137.217 kb on - strand, within GFF3861 at 137.406 kb on - strand at 137.488 kb on + strand, within GFF3862 at 137.526 kb on - strand, within GFF3862 at 137.555 kb on + strand, within GFF3862 at 137.693 kb on - strand, within GFF3862 at 137.895 kb on + strand, within GFF3862 at 137.896 kb on - strand, within GFF3862 at 137.897 kb on + strand, within GFF3862 at 138.104 kb on + strand at 138.106 kb on + strand at 138.107 kb on - strand at 138.107 kb on - strand at 138.174 kb on + strand at 138.190 kb on + strand at 138.398 kb on - strand, within GFF3863 at 138.949 kb on + strand, within GFF3863 at 138.950 kb on - strand, within GFF3863
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 136,388 - -0.3 136,388 - +0.8 136,393 - -0.9 136,393 - -0.2 136,396 - -0.9 136,430 + GFF3860 0.13 +0.7 136,586 + -0.0 136,623 + +0.1 136,623 + -2.5 136,623 + +0.2 136,624 - +0.2 136,797 + GFF3861 0.22 +0.5 136,800 + GFF3861 0.23 +0.7 136,801 - GFF3861 0.23 +1.0 136,848 + GFF3861 0.30 -0.1 136,849 - GFF3861 0.31 +0.9 136,935 + GFF3861 0.44 +0.0 136,935 + GFF3861 0.44 -0.1 136,936 - GFF3861 0.44 +0.3 136,941 + GFF3861 0.45 +0.2 136,942 - GFF3861 0.45 +1.0 136,997 + GFF3861 0.54 -1.3 137,009 + GFF3861 0.55 +0.1 137,209 - GFF3861 0.87 +0.7 137,217 - GFF3861 0.88 +0.7 137,406 - -0.6 137,488 + GFF3862 0.18 -3.9 137,526 - GFF3862 0.24 +0.6 137,555 + GFF3862 0.29 -2.2 137,693 - GFF3862 0.53 -0.3 137,895 + GFF3862 0.88 -3.7 137,896 - GFF3862 0.88 +1.0 137,897 + GFF3862 0.88 -2.6 138,104 + +2.2 138,106 + +1.5 138,107 - +1.8 138,107 - +2.4 138,174 + +1.9 138,190 + +1.9 138,398 - GFF3863 0.21 +2.8 138,949 + GFF3863 0.51 +1.0 138,950 - GFF3863 0.51 +1.5
Or see this region's nucleotide sequence