Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3408 and GFF3409 are separated by 93 nucleotides GFF3409 and GFF3410 overlap by 17 nucleotides GFF3410 and GFF3411 are separated by 250 nucleotides
GFF3408 - FIG01045299: hypothetical protein, at 172,965 to 173,141
GFF3408
GFF3409 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 173,235 to 174,098
GFF3409
GFF3410 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 174,082 to 175,218
GFF3410
GFF3411 - Tripeptide aminopeptidase (EC 3.4.11.4), at 175,469 to 176,698
GFF3411
Position (kb)
174
175
176 Strain fitness (log2 ratio)
-2
-1
0
1 at 173.244 kb on + strand at 173.374 kb on - strand, within GFF3409 at 173.746 kb on + strand, within GFF3409 at 173.942 kb on - strand, within GFF3409 at 174.036 kb on + strand at 174.037 kb on - strand at 174.037 kb on - strand at 174.132 kb on + strand at 174.298 kb on - strand, within GFF3410 at 174.322 kb on + strand, within GFF3410 at 174.386 kb on - strand, within GFF3410 at 174.386 kb on - strand, within GFF3410 at 174.386 kb on - strand, within GFF3410 at 174.387 kb on + strand, within GFF3410 at 174.388 kb on - strand, within GFF3410 at 174.710 kb on - strand, within GFF3410 at 174.878 kb on - strand, within GFF3410 at 174.943 kb on - strand, within GFF3410 at 174.947 kb on - strand, within GFF3410 at 175.099 kb on - strand, within GFF3410 at 175.220 kb on + strand at 175.335 kb on + strand at 175.336 kb on - strand at 175.435 kb on + strand at 175.435 kb on + strand at 175.613 kb on - strand, within GFF3411 at 175.618 kb on + strand, within GFF3411 at 175.624 kb on - strand, within GFF3411 at 175.624 kb on - strand, within GFF3411 at 175.776 kb on + strand, within GFF3411 at 176.046 kb on - strand, within GFF3411 at 176.047 kb on + strand, within GFF3411 at 176.048 kb on - strand, within GFF3411 at 176.048 kb on - strand, within GFF3411 at 176.048 kb on - strand, within GFF3411 at 176.048 kb on - strand, within GFF3411 at 176.129 kb on - strand, within GFF3411
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 173,244 + -0.1 173,374 - GFF3409 0.16 -0.7 173,746 + GFF3409 0.59 +0.5 173,942 - GFF3409 0.82 -0.6 174,036 + +1.0 174,037 - +0.2 174,037 - -0.4 174,132 + -0.2 174,298 - GFF3410 0.19 -0.1 174,322 + GFF3410 0.21 +0.1 174,386 - GFF3410 0.27 -0.6 174,386 - GFF3410 0.27 -0.3 174,386 - GFF3410 0.27 -1.2 174,387 + GFF3410 0.27 -0.5 174,388 - GFF3410 0.27 +0.0 174,710 - GFF3410 0.55 +0.1 174,878 - GFF3410 0.70 -0.5 174,943 - GFF3410 0.76 -2.1 174,947 - GFF3410 0.76 -0.2 175,099 - GFF3410 0.89 -0.6 175,220 + +0.0 175,335 + +0.4 175,336 - +0.4 175,435 + +1.3 175,435 + -0.4 175,613 - GFF3411 0.12 +0.6 175,618 + GFF3411 0.12 +0.1 175,624 - GFF3411 0.13 -0.4 175,624 - GFF3411 0.13 +0.7 175,776 + GFF3411 0.25 +0.8 176,046 - GFF3411 0.47 +0.3 176,047 + GFF3411 0.47 -0.1 176,048 - GFF3411 0.47 -0.7 176,048 - GFF3411 0.47 +0.7 176,048 - GFF3411 0.47 +1.3 176,048 - GFF3411 0.47 +0.1 176,129 - GFF3411 0.54 +0.4
Or see this region's nucleotide sequence