Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2805 and GFF2806 are separated by 46 nucleotides GFF2806 and GFF2807 are separated by 219 nucleotides GFF2807 and GFF2808 are separated by 192 nucleotides GFF2808 and GFF2809 are separated by 108 nucleotides
GFF2805 - FIG004684: SpoVR-like protein, at 329,579 to 331,111
GFF2805
GFF2806 - Transcriptional regulator for fatty acid degradation FadR, GntR family, at 331,158 to 331,877
GFF2806
GFF2807 - Na+/H+ antiporter NhaB, at 332,097 to 333,641
GFF2807
GFF2808 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 333,834 to 334,313
GFF2808
GFF2809 - FIG074102: hypothetical protein, at 334,422 to 334,763
GFF2809
Position (kb) 332
333
334 Strain fitness (log2 ratio) -1
0
1
2 at 331.282 kb on - strand, within GFF2806 at 331.291 kb on - strand, within GFF2806 at 331.343 kb on - strand, within GFF2806 at 331.363 kb on + strand, within GFF2806 at 331.547 kb on + strand, within GFF2806 at 331.557 kb on - strand, within GFF2806 at 331.833 kb on - strand at 331.834 kb on + strand at 332.171 kb on - strand at 332.303 kb on - strand, within GFF2807 at 332.350 kb on + strand, within GFF2807 at 332.351 kb on - strand, within GFF2807 at 332.351 kb on - strand, within GFF2807 at 332.416 kb on - strand, within GFF2807 at 332.416 kb on - strand, within GFF2807 at 332.420 kb on + strand, within GFF2807 at 332.590 kb on - strand, within GFF2807 at 332.659 kb on - strand, within GFF2807 at 332.659 kb on - strand, within GFF2807 at 332.848 kb on + strand, within GFF2807 at 332.995 kb on + strand, within GFF2807 at 333.263 kb on - strand, within GFF2807 at 333.274 kb on - strand, within GFF2807 at 333.274 kb on - strand, within GFF2807 at 333.330 kb on + strand, within GFF2807 at 333.516 kb on - strand at 333.542 kb on + strand at 333.543 kb on - strand at 333.565 kb on + strand at 333.698 kb on + strand at 333.698 kb on + strand at 333.699 kb on - strand at 333.829 kb on + strand at 333.910 kb on + strand, within GFF2808 at 333.910 kb on + strand, within GFF2808 at 333.910 kb on + strand, within GFF2808 at 333.914 kb on + strand, within GFF2808 at 333.914 kb on + strand, within GFF2808 at 333.918 kb on + strand, within GFF2808 at 333.918 kb on + strand, within GFF2808 at 334.017 kb on + strand, within GFF2808 at 334.036 kb on + strand, within GFF2808 at 334.055 kb on + strand, within GFF2808 at 334.267 kb on + strand at 334.421 kb on + strand at 334.422 kb on - strand at 334.432 kb on + strand at 334.432 kb on + strand at 334.433 kb on - strand at 334.467 kb on - strand, within GFF2809 at 334.572 kb on + strand, within GFF2809
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 331,282 - GFF2806 0.17 +2.4 331,291 - GFF2806 0.18 +1.9 331,343 - GFF2806 0.26 +0.9 331,363 + GFF2806 0.28 +0.7 331,547 + GFF2806 0.54 +0.6 331,557 - GFF2806 0.55 +1.9 331,833 - +1.8 331,834 + +1.2 332,171 - -0.7 332,303 - GFF2807 0.13 -0.7 332,350 + GFF2807 0.16 +0.4 332,351 - GFF2807 0.16 +0.0 332,351 - GFF2807 0.16 -0.7 332,416 - GFF2807 0.21 +0.9 332,416 - GFF2807 0.21 -0.1 332,420 + GFF2807 0.21 -0.7 332,590 - GFF2807 0.32 +0.6 332,659 - GFF2807 0.36 +0.0 332,659 - GFF2807 0.36 +0.0 332,848 + GFF2807 0.49 -0.0 332,995 + GFF2807 0.58 -0.3 333,263 - GFF2807 0.75 -0.7 333,274 - GFF2807 0.76 -0.8 333,274 - GFF2807 0.76 -0.2 333,330 + GFF2807 0.80 -0.6 333,516 - +0.1 333,542 + -1.7 333,543 - -0.4 333,565 + +0.0 333,698 + +0.4 333,698 + -0.3 333,699 - +0.1 333,829 + +1.2 333,910 + GFF2808 0.16 +1.2 333,910 + GFF2808 0.16 +1.1 333,910 + GFF2808 0.16 +1.6 333,914 + GFF2808 0.17 +0.6 333,914 + GFF2808 0.17 +1.0 333,918 + GFF2808 0.17 +1.2 333,918 + GFF2808 0.17 +1.8 334,017 + GFF2808 0.38 +1.1 334,036 + GFF2808 0.42 +1.8 334,055 + GFF2808 0.46 +1.0 334,267 + -0.4 334,421 + +0.1 334,422 - -0.2 334,432 + +0.0 334,432 + -0.0 334,433 - +0.2 334,467 - GFF2809 0.13 -0.1 334,572 + GFF2809 0.44 +0.0
Or see this region's nucleotide sequence