Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2801

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2799 and GFF2800 are separated by 13 nucleotidesGFF2800 and GFF2801 are separated by 170 nucleotidesGFF2801 and GFF2802 are separated by 96 nucleotides GFF2799 - Inner membrane protein, at 322,537 to 323,271 GFF2799 GFF2800 - Membrane-bound lytic murein transglycosylase E (EC 3.2.1.-), at 323,285 to 323,896 GFF2800 GFF2801 - Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13), at 324,067 to 324,981 GFF2801 GFF2802 - Cell volume regulation protein A, at 325,078 to 326,811 GFF2802 Position (kb) 324 325Strain fitness (log2 ratio) -1 0 1 2at 323.282 kb on - strandat 323.461 kb on - strand, within GFF2800at 323.461 kb on - strand, within GFF2800at 323.861 kb on + strandat 323.862 kb on - strandat 323.865 kb on - strandat 323.865 kb on - strandat 323.904 kb on - strandat 323.922 kb on + strandat 323.923 kb on - strandat 323.960 kb on + strandat 323.961 kb on - strandat 323.961 kb on - strandat 323.961 kb on - strandat 324.014 kb on + strandat 324.017 kb on + strandat 324.034 kb on - strandat 324.047 kb on - strandat 324.047 kb on - strandat 324.064 kb on - strandat 324.448 kb on - strand, within GFF2801at 324.483 kb on + strand, within GFF2801at 324.483 kb on + strand, within GFF2801at 324.484 kb on - strand, within GFF2801at 324.699 kb on + strand, within GFF2801at 324.700 kb on - strand, within GFF2801at 324.953 kb on + strandat 324.967 kb on - strandat 325.000 kb on + strandat 325.043 kb on - strandat 325.112 kb on + strandat 325.145 kb on + strandat 325.146 kb on - strandat 325.245 kb on + strandat 325.262 kb on - strand, within GFF2802at 325.262 kb on - strand, within GFF2802at 325.264 kb on - strand, within GFF2802at 325.264 kb on - strand, within GFF2802at 325.587 kb on + strand, within GFF2802at 325.722 kb on + strand, within GFF2802

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
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323,282 - -0.2
323,461 - GFF2800 0.29 +0.3
323,461 - GFF2800 0.29 -0.2
323,861 + -0.0
323,862 - +0.2
323,865 - +0.2
323,865 - -0.4
323,904 - +0.2
323,922 + -0.5
323,923 - +0.3
323,960 + +0.3
323,961 - -0.2
323,961 - -0.2
323,961 - +0.3
324,014 + -0.1
324,017 + +0.0
324,034 - +0.3
324,047 - +0.5
324,047 - +0.1
324,064 - +1.1
324,448 - GFF2801 0.42 +1.3
324,483 + GFF2801 0.45 +0.7
324,483 + GFF2801 0.45 +0.9
324,484 - GFF2801 0.46 +1.2
324,699 + GFF2801 0.69 +1.8
324,700 - GFF2801 0.69 -0.2
324,953 + +1.6
324,967 - +1.0
325,000 + -0.2
325,043 - -0.1
325,112 + -0.1
325,145 + -0.3
325,146 - -0.2
325,245 + -1.5
325,262 - GFF2802 0.11 -0.2
325,262 - GFF2802 0.11 +0.2
325,264 - GFF2802 0.11 -0.7
325,264 - GFF2802 0.11 -0.1
325,587 + GFF2802 0.29 -0.2
325,722 + GFF2802 0.37 +0.1

Or see this region's nucleotide sequence