Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2754 and GFF2755 are separated by 218 nucleotides GFF2755 and GFF2756 are separated by 50 nucleotides GFF2756 and GFF2874 are separated by 158 nucleotides GFF2874 and GFF2875 are separated by 204 nucleotides GFF2875 and GFF2757 are separated by 434 nucleotides
GFF2754 - UPF0028 protein YchK, at 275,661 to 276,557
GFF2754
GFF2755 - UPF0225 protein YchJ, at 276,776 to 277,135
GFF2755
GFF2756 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 277,186 to 278,028
GFF2756
GFF2874 - tRNA-Tyr-GTA, at 278,187 to 278,271
GFF2874
GFF2875 - tRNA-Tyr-GTA, at 278,476 to 278,560
GFF2875
GFF2757 - Putative secreted protein, at 278,995 to 280,524
GFF2757
Position (kb) 277
278
279 Strain fitness (log2 ratio) -2
-1
0
1
2 at 276.194 kb on + strand, within GFF2754 at 276.202 kb on - strand, within GFF2754 at 276.282 kb on - strand, within GFF2754 at 276.339 kb on + strand, within GFF2754 at 276.339 kb on + strand, within GFF2754 at 276.381 kb on + strand, within GFF2754 at 276.468 kb on - strand at 276.569 kb on + strand at 276.569 kb on + strand at 276.600 kb on - strand at 276.623 kb on - strand at 276.628 kb on - strand at 276.673 kb on + strand at 276.674 kb on - strand at 276.713 kb on + strand at 276.714 kb on - strand at 276.715 kb on + strand at 276.720 kb on + strand at 276.720 kb on + strand at 276.720 kb on + strand at 276.720 kb on + strand at 276.721 kb on - strand at 276.721 kb on - strand at 276.754 kb on - strand at 276.823 kb on - strand, within GFF2755 at 276.925 kb on + strand, within GFF2755 at 276.926 kb on - strand, within GFF2755 at 276.953 kb on + strand, within GFF2755 at 277.046 kb on + strand, within GFF2755 at 277.047 kb on - strand, within GFF2755 at 277.048 kb on + strand, within GFF2755 at 277.197 kb on - strand at 277.218 kb on + strand at 277.270 kb on - strand at 277.504 kb on + strand, within GFF2756 at 277.505 kb on - strand, within GFF2756 at 277.690 kb on + strand, within GFF2756 at 277.693 kb on - strand, within GFF2756 at 277.852 kb on - strand, within GFF2756 at 277.852 kb on - strand, within GFF2756 at 278.040 kb on - strand at 278.179 kb on + strand at 278.290 kb on + strand at 278.352 kb on + strand at 278.595 kb on + strand at 278.749 kb on + strand at 278.750 kb on - strand at 278.779 kb on + strand at 278.989 kb on - strand at 278.999 kb on + strand at 278.999 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 276,194 + GFF2754 0.59 +0.4 276,202 - GFF2754 0.60 +0.4 276,282 - GFF2754 0.69 +1.3 276,339 + GFF2754 0.76 +1.9 276,339 + GFF2754 0.76 +1.5 276,381 + GFF2754 0.80 +0.7 276,468 - +0.8 276,569 + +2.2 276,569 + +1.7 276,600 - -2.2 276,623 - -1.8 276,628 - -2.5 276,673 + +1.4 276,674 - -1.0 276,713 + +1.4 276,714 - -0.9 276,715 + +0.9 276,720 + +0.7 276,720 + +0.6 276,720 + +0.8 276,720 + +0.8 276,721 - -1.6 276,721 - -1.6 276,754 - -1.7 276,823 - GFF2755 0.13 -2.0 276,925 + GFF2755 0.41 +0.5 276,926 - GFF2755 0.42 -0.5 276,953 + GFF2755 0.49 -0.8 277,046 + GFF2755 0.75 -0.3 277,047 - GFF2755 0.75 -0.5 277,048 + GFF2755 0.76 -0.3 277,197 - -0.5 277,218 + +0.3 277,270 - -0.7 277,504 + GFF2756 0.38 +0.3 277,505 - GFF2756 0.38 +0.2 277,690 + GFF2756 0.60 +0.2 277,693 - GFF2756 0.60 -1.1 277,852 - GFF2756 0.79 -0.1 277,852 - GFF2756 0.79 -0.6 278,040 - -0.3 278,179 + -0.2 278,290 + -0.0 278,352 + +0.0 278,595 + +0.2 278,749 + -0.2 278,750 - -0.1 278,779 + +0.1 278,989 - +0.0 278,999 + +1.6 278,999 + -0.4
Or see this region's nucleotide sequence