Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2316

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2315 and GFF2316 are separated by 186 nucleotidesGFF2316 and GFF2317 are separated by 154 nucleotides GFF2315 - Cell division protein FtsN, at 100,037 to 100,918 GFF2315 GFF2316 - Transcriptional (co)regulator CytR, at 101,105 to 102,130 GFF2316 GFF2317 - Helicase PriA essential for oriC/DnaA-independent DNA replication, at 102,285 to 104,483 GFF2317 Position (kb) 101 102 103Strain fitness (log2 ratio) -1 0 1at 101.102 kb on - strandat 101.123 kb on - strandat 101.366 kb on + strand, within GFF2316at 101.450 kb on - strand, within GFF2316at 101.469 kb on + strand, within GFF2316at 101.470 kb on - strand, within GFF2316at 101.470 kb on - strand, within GFF2316at 101.470 kb on - strand, within GFF2316at 101.501 kb on + strand, within GFF2316at 101.514 kb on + strand, within GFF2316at 101.515 kb on - strand, within GFF2316at 101.523 kb on - strand, within GFF2316at 101.636 kb on - strand, within GFF2316at 101.636 kb on - strand, within GFF2316at 101.747 kb on + strand, within GFF2316at 101.748 kb on - strand, within GFF2316at 101.783 kb on - strand, within GFF2316at 102.064 kb on + strandat 102.109 kb on + strandat 102.175 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
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101,102 - -0.5
101,123 - -0.1
101,366 + GFF2316 0.25 -0.3
101,450 - GFF2316 0.34 -0.7
101,469 + GFF2316 0.35 -0.8
101,470 - GFF2316 0.36 +1.0
101,470 - GFF2316 0.36 -0.8
101,470 - GFF2316 0.36 -1.0
101,501 + GFF2316 0.39 -0.1
101,514 + GFF2316 0.40 -0.2
101,515 - GFF2316 0.40 -1.1
101,523 - GFF2316 0.41 -0.4
101,636 - GFF2316 0.52 -0.2
101,636 - GFF2316 0.52 -0.4
101,747 + GFF2316 0.63 -0.1
101,748 - GFF2316 0.63 -0.4
101,783 - GFF2316 0.66 -1.2
102,064 + -0.1
102,109 + +1.2
102,175 + -0.3

Or see this region's nucleotide sequence