Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1542

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1541 and GFF1542 are separated by 136 nucleotidesGFF1542 and GFF1543 are separated by 91 nucleotidesGFF1543 and GFF1544 overlap by 48 nucleotides GFF1541 - Flagellar synthesis: repressor of fliC, at 805 to 1,275 GFF1541 GFF1542 - Flagellar biosynthesis protein FliC, at 1,412 to 2,932 GFF1542 GFF1543 - DNA-invertase, at 3,024 to 3,596 GFF1543 GFF1544 - hypothetical protein, at 3,549 to 3,686 GFF1544 Position (kb) 1 2 3Strain fitness (log2 ratio) -1 0 1at 0.427 kb on + strandat 0.492 kb on + strandat 0.571 kb on - strandat 0.571 kb on - strandat 0.620 kb on - strandat 0.639 kb on - strandat 0.673 kb on + strandat 0.719 kb on - strandat 0.861 kb on + strand, within GFF1541at 0.938 kb on + strand, within GFF1541at 1.569 kb on - strand, within GFF1542at 1.850 kb on - strand, within GFF1542at 1.851 kb on + strand, within GFF1542at 1.864 kb on + strand, within GFF1542at 1.908 kb on + strand, within GFF1542at 1.967 kb on - strand, within GFF1542at 2.027 kb on + strand, within GFF1542at 2.072 kb on + strand, within GFF1542at 2.072 kb on + strand, within GFF1542at 2.081 kb on + strand, within GFF1542at 2.082 kb on - strand, within GFF1542at 2.176 kb on - strand, within GFF1542at 2.218 kb on + strand, within GFF1542at 2.230 kb on - strand, within GFF1542at 2.230 kb on - strand, within GFF1542at 2.299 kb on + strand, within GFF1542at 2.334 kb on - strand, within GFF1542at 2.348 kb on - strand, within GFF1542at 2.398 kb on - strand, within GFF1542at 2.922 kb on - strandat 3.002 kb on - strandat 3.032 kb on + strandat 3.093 kb on - strand, within GFF1543at 3.317 kb on + strand, within GFF1543at 3.546 kb on - strandat 3.550 kb on + strandat 3.722 kb on + strandat 3.726 kb on + strandat 3.726 kb on + strandat 3.911 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
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427 + -0.5
492 + -0.2
571 - +0.5
571 - +0.9
620 - +0.0
639 - +0.1
673 + +0.3
719 - +0.4
861 + GFF1541 0.12 +0.1
938 + GFF1541 0.28 +0.0
1,569 - GFF1542 0.10 +0.0
1,850 - GFF1542 0.29 +0.1
1,851 + GFF1542 0.29 +0.4
1,864 + GFF1542 0.30 +0.3
1,908 + GFF1542 0.33 -0.1
1,967 - GFF1542 0.36 +0.2
2,027 + GFF1542 0.40 +0.1
2,072 + GFF1542 0.43 +0.4
2,072 + GFF1542 0.43 +0.6
2,081 + GFF1542 0.44 -0.0
2,082 - GFF1542 0.44 +0.7
2,176 - GFF1542 0.50 +0.9
2,218 + GFF1542 0.53 +0.3
2,230 - GFF1542 0.54 +0.4
2,230 - GFF1542 0.54 +0.2
2,299 + GFF1542 0.58 +0.3
2,334 - GFF1542 0.61 +0.2
2,348 - GFF1542 0.62 +0.1
2,398 - GFF1542 0.65 +0.6
2,922 - +0.1
3,002 - +1.1
3,032 + +0.6
3,093 - GFF1543 0.12 +0.3
3,317 + GFF1543 0.51 -0.1
3,546 - +0.3
3,550 + +0.3
3,722 + +0.7
3,726 + -0.4
3,726 + -0.5
3,911 - -0.1

Or see this region's nucleotide sequence