Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1541 and GFF1542 are separated by 136 nucleotides GFF1542 and GFF1543 are separated by 91 nucleotides GFF1543 and GFF1544 overlap by 48 nucleotides
GFF1541 - Flagellar synthesis: repressor of fliC, at 805 to 1,275
GFF1541
GFF1542 - Flagellar biosynthesis protein FliC, at 1,412 to 2,932
GFF1542
GFF1543 - DNA-invertase, at 3,024 to 3,596
GFF1543
GFF1544 - hypothetical protein, at 3,549 to 3,686
GFF1544
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-1
0
1 at 0.427 kb on + strand at 0.492 kb on + strand at 0.571 kb on - strand at 0.571 kb on - strand at 0.620 kb on - strand at 0.639 kb on - strand at 0.673 kb on + strand at 0.719 kb on - strand at 0.861 kb on + strand, within GFF1541 at 0.938 kb on + strand, within GFF1541 at 1.569 kb on - strand, within GFF1542 at 1.850 kb on - strand, within GFF1542 at 1.851 kb on + strand, within GFF1542 at 1.864 kb on + strand, within GFF1542 at 1.908 kb on + strand, within GFF1542 at 1.967 kb on - strand, within GFF1542 at 2.027 kb on + strand, within GFF1542 at 2.072 kb on + strand, within GFF1542 at 2.072 kb on + strand, within GFF1542 at 2.081 kb on + strand, within GFF1542 at 2.082 kb on - strand, within GFF1542 at 2.176 kb on - strand, within GFF1542 at 2.218 kb on + strand, within GFF1542 at 2.230 kb on - strand, within GFF1542 at 2.230 kb on - strand, within GFF1542 at 2.299 kb on + strand, within GFF1542 at 2.334 kb on - strand, within GFF1542 at 2.348 kb on - strand, within GFF1542 at 2.398 kb on - strand, within GFF1542 at 2.922 kb on - strand at 3.002 kb on - strand at 3.032 kb on + strand at 3.093 kb on - strand, within GFF1543 at 3.317 kb on + strand, within GFF1543 at 3.546 kb on - strand at 3.550 kb on + strand at 3.722 kb on + strand at 3.726 kb on + strand at 3.726 kb on + strand at 3.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 427 + -0.5 492 + -0.2 571 - +0.5 571 - +0.9 620 - +0.0 639 - +0.1 673 + +0.3 719 - +0.4 861 + GFF1541 0.12 +0.1 938 + GFF1541 0.28 +0.0 1,569 - GFF1542 0.10 +0.0 1,850 - GFF1542 0.29 +0.1 1,851 + GFF1542 0.29 +0.4 1,864 + GFF1542 0.30 +0.3 1,908 + GFF1542 0.33 -0.1 1,967 - GFF1542 0.36 +0.2 2,027 + GFF1542 0.40 +0.1 2,072 + GFF1542 0.43 +0.4 2,072 + GFF1542 0.43 +0.6 2,081 + GFF1542 0.44 -0.0 2,082 - GFF1542 0.44 +0.7 2,176 - GFF1542 0.50 +0.9 2,218 + GFF1542 0.53 +0.3 2,230 - GFF1542 0.54 +0.4 2,230 - GFF1542 0.54 +0.2 2,299 + GFF1542 0.58 +0.3 2,334 - GFF1542 0.61 +0.2 2,348 - GFF1542 0.62 +0.1 2,398 - GFF1542 0.65 +0.6 2,922 - +0.1 3,002 - +1.1 3,032 + +0.6 3,093 - GFF1543 0.12 +0.3 3,317 + GFF1543 0.51 -0.1 3,546 - +0.3 3,550 + +0.3 3,722 + +0.7 3,726 + -0.4 3,726 + -0.5 3,911 - -0.1
Or see this region's nucleotide sequence