Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF134 and GFF135 are separated by 225 nucleotides GFF135 and GFF136 are separated by 41 nucleotides GFF136 and GFF137 are separated by 9 nucleotides GFF137 and GFF138 are separated by 153 nucleotides GFF138 and GFF139 are separated by 33 nucleotides
GFF134 - GMP reductase (EC 1.7.1.7), at 123,994 to 125,037
GFF134
GFF135 - Dephospho-CoA kinase (EC 2.7.1.24), at 125,263 to 125,883
GFF135
GFF136 - FIG002842: hypothetical protein, at 125,925 to 126,626
GFF136
GFF137 - FIG003276: zinc-binding protein, at 126,636 to 126,827
GFF137
GFF138 - Aldo-keto reductase, at 126,981 to 127,115
GFF138
GFF139 - Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-), at 127,149 to 127,544
GFF139
Position (kb)
125
126
127 Strain fitness (log2 ratio)
-1
0
1
2 at 125.040 kb on - strand at 125.040 kb on - strand at 125.093 kb on + strand at 125.098 kb on + strand at 125.099 kb on - strand at 125.178 kb on + strand at 125.198 kb on - strand at 125.262 kb on + strand at 125.930 kb on + strand at 125.931 kb on - strand at 126.167 kb on - strand, within GFF136 at 126.167 kb on - strand, within GFF136 at 126.261 kb on - strand, within GFF136 at 126.302 kb on + strand, within GFF136 at 126.303 kb on - strand, within GFF136 at 126.303 kb on - strand, within GFF136 at 126.491 kb on + strand, within GFF136 at 126.492 kb on - strand, within GFF136 at 126.537 kb on - strand, within GFF136 at 126.537 kb on - strand, within GFF136 at 126.537 kb on - strand, within GFF136 at 126.655 kb on + strand at 126.924 kb on + strand at 126.944 kb on + strand at 126.949 kb on + strand at 127.105 kb on + strand at 127.117 kb on - strand at 127.305 kb on - strand, within GFF139 at 127.364 kb on + strand, within GFF139 at 127.383 kb on + strand, within GFF139 at 127.383 kb on + strand, within GFF139 at 127.384 kb on - strand, within GFF139
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 125,040 - +0.4 125,040 - +0.2 125,093 + +0.0 125,098 + +0.0 125,099 - -0.1 125,178 + -0.5 125,198 - +1.8 125,262 + -0.8 125,930 + +0.6 125,931 - +0.5 126,167 - GFF136 0.34 +0.6 126,167 - GFF136 0.34 +0.8 126,261 - GFF136 0.48 +1.0 126,302 + GFF136 0.54 +0.3 126,303 - GFF136 0.54 +0.7 126,303 - GFF136 0.54 +1.3 126,491 + GFF136 0.81 +0.5 126,492 - GFF136 0.81 +0.6 126,537 - GFF136 0.87 +0.4 126,537 - GFF136 0.87 +1.0 126,537 - GFF136 0.87 +0.6 126,655 + +0.5 126,924 + -0.4 126,944 + +0.2 126,949 + +0.2 127,105 + -0.1 127,117 - -0.9 127,305 - GFF139 0.39 -0.1 127,364 + GFF139 0.54 -0.4 127,383 + GFF139 0.59 +0.0 127,383 + GFF139 0.59 +0.3 127,384 - GFF139 0.59 +0.1
Or see this region's nucleotide sequence