Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1210 and GFF1211 are separated by 14 nucleotides GFF1211 and GFF1212 overlap by 8 nucleotides GFF1212 and GFF1213 are separated by 83 nucleotides GFF1213 and GFF1214 are separated by 27 nucleotides
GFF1210 - Kappa-fimbriae probable subunit, at 49,899 to 50,456
GFF1210
GFF1211 - Kappa-fimbriae chaperone protein, at 50,471 to 51,163
GFF1211
GFF1212 - Kappa-fimbriae usher protein, at 51,156 to 53,564
GFF1212
GFF1213 - hypothetical protein, at 53,648 to 53,764
GFF1213
GFF1214 - Kappa-fimbriae major subunit, at 53,792 to 54,310
GFF1214
Position (kb)
51
52
53
54 Strain fitness (log2 ratio)
-2
-1
0
1 at 50.192 kb on + strand, within GFF1210 at 50.193 kb on - strand, within GFF1210 at 50.269 kb on + strand, within GFF1210 at 50.270 kb on - strand, within GFF1210 at 50.279 kb on + strand, within GFF1210 at 50.280 kb on - strand, within GFF1210 at 50.283 kb on - strand, within GFF1210 at 50.349 kb on - strand, within GFF1210 at 50.485 kb on - strand at 50.485 kb on - strand at 50.930 kb on - strand, within GFF1211 at 50.955 kb on + strand, within GFF1211 at 50.955 kb on + strand, within GFF1211 at 50.955 kb on + strand, within GFF1211 at 50.956 kb on - strand, within GFF1211 at 50.956 kb on - strand, within GFF1211 at 50.956 kb on - strand, within GFF1211 at 50.956 kb on - strand, within GFF1211 at 50.956 kb on - strand, within GFF1211 at 51.064 kb on - strand, within GFF1211 at 51.157 kb on + strand at 51.157 kb on + strand at 51.158 kb on - strand at 51.158 kb on - strand at 51.166 kb on + strand at 51.192 kb on - strand at 51.201 kb on - strand at 51.201 kb on - strand at 51.272 kb on + strand at 51.273 kb on - strand at 51.273 kb on - strand at 51.385 kb on + strand at 51.521 kb on - strand, within GFF1212 at 51.545 kb on - strand, within GFF1212 at 51.545 kb on - strand, within GFF1212 at 51.545 kb on - strand, within GFF1212 at 51.704 kb on + strand, within GFF1212 at 51.901 kb on + strand, within GFF1212 at 51.902 kb on - strand, within GFF1212 at 51.935 kb on + strand, within GFF1212 at 51.955 kb on + strand, within GFF1212 at 51.974 kb on - strand, within GFF1212 at 51.983 kb on + strand, within GFF1212 at 51.984 kb on - strand, within GFF1212 at 51.988 kb on + strand, within GFF1212 at 51.988 kb on + strand, within GFF1212 at 51.988 kb on + strand, within GFF1212 at 51.989 kb on - strand, within GFF1212 at 51.989 kb on - strand, within GFF1212 at 52.032 kb on - strand, within GFF1212 at 52.078 kb on + strand, within GFF1212 at 52.079 kb on - strand, within GFF1212 at 52.079 kb on - strand, within GFF1212 at 52.109 kb on + strand, within GFF1212 at 52.162 kb on + strand, within GFF1212 at 52.182 kb on - strand, within GFF1212 at 52.187 kb on - strand, within GFF1212 at 52.208 kb on - strand, within GFF1212 at 52.273 kb on + strand, within GFF1212 at 52.273 kb on + strand, within GFF1212 at 52.273 kb on + strand, within GFF1212 at 52.402 kb on + strand, within GFF1212 at 52.403 kb on - strand, within GFF1212 at 52.514 kb on + strand, within GFF1212 at 52.514 kb on + strand, within GFF1212 at 52.515 kb on - strand, within GFF1212 at 52.515 kb on - strand, within GFF1212 at 52.515 kb on - strand, within GFF1212 at 52.516 kb on + strand, within GFF1212 at 52.516 kb on + strand, within GFF1212 at 52.516 kb on + strand, within GFF1212 at 52.517 kb on - strand, within GFF1212 at 52.520 kb on + strand, within GFF1212 at 52.549 kb on + strand, within GFF1212 at 52.550 kb on - strand, within GFF1212 at 52.550 kb on - strand, within GFF1212 at 52.550 kb on - strand, within GFF1212 at 52.550 kb on - strand, within GFF1212 at 52.685 kb on - strand, within GFF1212 at 52.838 kb on - strand, within GFF1212 at 52.985 kb on + strand, within GFF1212 at 52.985 kb on + strand, within GFF1212 at 52.986 kb on - strand, within GFF1212 at 53.036 kb on - strand, within GFF1212 at 53.081 kb on - strand, within GFF1212 at 53.107 kb on + strand, within GFF1212 at 53.108 kb on - strand, within GFF1212 at 53.203 kb on + strand, within GFF1212 at 53.204 kb on - strand, within GFF1212 at 53.224 kb on + strand, within GFF1212 at 53.224 kb on + strand, within GFF1212 at 53.224 kb on + strand, within GFF1212 at 53.225 kb on - strand, within GFF1212 at 53.225 kb on - strand, within GFF1212 at 53.258 kb on - strand, within GFF1212 at 53.395 kb on + strand at 53.395 kb on + strand at 53.395 kb on + strand at 53.396 kb on - strand at 53.396 kb on - strand at 53.396 kb on - strand at 53.396 kb on - strand at 53.430 kb on + strand at 53.969 kb on + strand, within GFF1214 at 53.983 kb on + strand, within GFF1214 at 53.983 kb on + strand, within GFF1214 at 54.284 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 50,192 + GFF1210 0.53 +0.0 50,193 - GFF1210 0.53 +0.1 50,269 + GFF1210 0.66 -0.2 50,270 - GFF1210 0.66 +0.6 50,279 + GFF1210 0.68 -0.3 50,280 - GFF1210 0.68 -1.0 50,283 - GFF1210 0.69 +0.5 50,349 - GFF1210 0.81 -0.2 50,485 - -0.1 50,485 - -0.7 50,930 - GFF1211 0.66 +0.7 50,955 + GFF1211 0.70 +0.3 50,955 + GFF1211 0.70 +0.2 50,955 + GFF1211 0.70 +0.2 50,956 - GFF1211 0.70 -0.4 50,956 - GFF1211 0.70 -0.5 50,956 - GFF1211 0.70 -0.3 50,956 - GFF1211 0.70 +0.3 50,956 - GFF1211 0.70 +0.1 51,064 - GFF1211 0.86 -0.6 51,157 + +0.2 51,157 + -0.5 51,158 - +0.6 51,158 - -0.6 51,166 + +0.7 51,192 - +0.5 51,201 - -0.0 51,201 - +0.2 51,272 + -0.2 51,273 - +1.7 51,273 - -0.9 51,385 + -0.1 51,521 - GFF1212 0.15 +0.6 51,545 - GFF1212 0.16 -0.8 51,545 - GFF1212 0.16 +0.0 51,545 - GFF1212 0.16 +0.2 51,704 + GFF1212 0.23 -0.1 51,901 + GFF1212 0.31 +0.1 51,902 - GFF1212 0.31 -0.5 51,935 + GFF1212 0.32 +0.3 51,955 + GFF1212 0.33 -0.5 51,974 - GFF1212 0.34 -0.9 51,983 + GFF1212 0.34 +0.4 51,984 - GFF1212 0.34 +0.1 51,988 + GFF1212 0.35 -0.1 51,988 + GFF1212 0.35 -0.1 51,988 + GFF1212 0.35 -0.4 51,989 - GFF1212 0.35 -0.4 51,989 - GFF1212 0.35 -0.8 52,032 - GFF1212 0.36 -2.6 52,078 + GFF1212 0.38 -2.0 52,079 - GFF1212 0.38 -0.3 52,079 - GFF1212 0.38 -0.8 52,109 + GFF1212 0.40 +0.5 52,162 + GFF1212 0.42 +0.2 52,182 - GFF1212 0.43 -0.3 52,187 - GFF1212 0.43 -0.6 52,208 - GFF1212 0.44 +0.6 52,273 + GFF1212 0.46 +0.4 52,273 + GFF1212 0.46 +0.1 52,273 + GFF1212 0.46 +0.1 52,402 + GFF1212 0.52 -0.9 52,403 - GFF1212 0.52 -0.6 52,514 + GFF1212 0.56 +0.0 52,514 + GFF1212 0.56 -0.1 52,515 - GFF1212 0.56 -2.0 52,515 - GFF1212 0.56 +0.4 52,515 - GFF1212 0.56 -0.4 52,516 + GFF1212 0.56 +0.2 52,516 + GFF1212 0.56 -1.1 52,516 + GFF1212 0.56 +0.9 52,517 - GFF1212 0.56 -0.5 52,520 + GFF1212 0.57 -0.1 52,549 + GFF1212 0.58 +0.9 52,550 - GFF1212 0.58 -0.1 52,550 - GFF1212 0.58 -0.0 52,550 - GFF1212 0.58 -1.5 52,550 - GFF1212 0.58 +0.1 52,685 - GFF1212 0.63 -0.6 52,838 - GFF1212 0.70 -0.1 52,985 + GFF1212 0.76 -0.4 52,985 + GFF1212 0.76 +0.7 52,986 - GFF1212 0.76 +0.9 53,036 - GFF1212 0.78 -0.4 53,081 - GFF1212 0.80 -0.2 53,107 + GFF1212 0.81 -0.1 53,108 - GFF1212 0.81 +0.4 53,203 + GFF1212 0.85 -0.7 53,204 - GFF1212 0.85 -1.2 53,224 + GFF1212 0.86 -0.1 53,224 + GFF1212 0.86 -0.6 53,224 + GFF1212 0.86 +0.2 53,225 - GFF1212 0.86 +1.0 53,225 - GFF1212 0.86 -1.1 53,258 - GFF1212 0.87 -0.2 53,395 + +0.2 53,395 + -0.5 53,395 + +0.3 53,396 - +0.3 53,396 - +0.1 53,396 - +0.1 53,396 - -0.5 53,430 + -0.1 53,969 + GFF1214 0.34 +0.1 53,983 + GFF1214 0.37 +0.5 53,983 + GFF1214 0.37 -0.4 54,284 - -0.1
Or see this region's nucleotide sequence