Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1044 and GFF1045 are separated by 51 nucleotides GFF1045 and GFF1046 are separated by 209 nucleotides GFF1046 and GFF1047 are separated by 37 nucleotides
GFF1044 - Thymidine phosphorylase (EC 2.4.2.4), at 225,686 to 227,008
GFF1044
GFF1045 - Phosphopentomutase (EC 5.4.2.7), at 227,060 to 228,283
GFF1045
GFF1046 - Purine nucleoside phosphorylase (EC 2.4.2.1), at 228,493 to 229,212
GFF1046
GFF1047 - Beta-fimbriae probable major subunit, at 229,250 to 229,822
GFF1047
Position (kb)
227
228
229 Strain fitness (log2 ratio)
-1
0
1
2 at 226.294 kb on - strand, within GFF1044 at 226.325 kb on + strand, within GFF1044 at 226.514 kb on - strand, within GFF1044 at 226.673 kb on + strand, within GFF1044 at 226.682 kb on + strand, within GFF1044 at 226.683 kb on - strand, within GFF1044 at 226.683 kb on - strand, within GFF1044 at 226.713 kb on + strand, within GFF1044 at 226.713 kb on + strand, within GFF1044 at 226.715 kb on + strand, within GFF1044 at 226.716 kb on - strand, within GFF1044 at 226.716 kb on - strand, within GFF1044 at 226.949 kb on - strand at 227.006 kb on + strand at 227.007 kb on - strand at 227.012 kb on + strand at 227.012 kb on + strand at 227.013 kb on - strand at 227.075 kb on - strand at 227.075 kb on - strand at 227.449 kb on + strand, within GFF1045 at 227.631 kb on - strand, within GFF1045 at 227.675 kb on + strand, within GFF1045 at 227.849 kb on + strand, within GFF1045 at 227.850 kb on - strand, within GFF1045 at 227.850 kb on - strand, within GFF1045 at 228.128 kb on - strand, within GFF1045 at 228.192 kb on + strand at 228.248 kb on - strand at 228.306 kb on - strand at 228.341 kb on + strand at 228.341 kb on + strand at 228.342 kb on - strand at 228.342 kb on - strand at 228.342 kb on - strand at 228.345 kb on + strand at 228.345 kb on + strand at 228.346 kb on - strand at 228.346 kb on - strand at 228.350 kb on + strand at 228.406 kb on + strand at 228.507 kb on + strand at 228.507 kb on + strand at 228.508 kb on - strand at 228.542 kb on + strand at 228.542 kb on + strand at 228.632 kb on + strand, within GFF1046 at 228.650 kb on - strand, within GFF1046 at 228.814 kb on - strand, within GFF1046 at 228.855 kb on + strand, within GFF1046 at 228.974 kb on - strand, within GFF1046 at 229.028 kb on + strand, within GFF1046 at 229.047 kb on + strand, within GFF1046 at 229.117 kb on - strand, within GFF1046 at 229.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.075 MOI remove 226,294 - GFF1044 0.46 +0.0 226,325 + GFF1044 0.48 -0.0 226,514 - GFF1044 0.63 +0.6 226,673 + GFF1044 0.75 -0.5 226,682 + GFF1044 0.75 -0.2 226,683 - GFF1044 0.75 -0.5 226,683 - GFF1044 0.75 +0.4 226,713 + GFF1044 0.78 -0.2 226,713 + GFF1044 0.78 -0.4 226,715 + GFF1044 0.78 -0.1 226,716 - GFF1044 0.78 +0.1 226,716 - GFF1044 0.78 +1.0 226,949 - +0.4 227,006 + +0.8 227,007 - +0.5 227,012 + +0.0 227,012 + -0.1 227,013 - +0.3 227,075 - +0.4 227,075 - -0.4 227,449 + GFF1045 0.32 -0.2 227,631 - GFF1045 0.47 +1.0 227,675 + GFF1045 0.50 +0.9 227,849 + GFF1045 0.64 +0.5 227,850 - GFF1045 0.65 -0.1 227,850 - GFF1045 0.65 +0.7 228,128 - GFF1045 0.87 +1.5 228,192 + +0.3 228,248 - +2.0 228,306 - +1.5 228,341 + -0.0 228,341 + -0.1 228,342 - +0.6 228,342 - +0.2 228,342 - +0.6 228,345 + -0.2 228,345 + -0.1 228,346 - +0.3 228,346 - +1.0 228,350 + -0.6 228,406 + +0.1 228,507 + +1.2 228,507 + +1.2 228,508 - +0.8 228,542 + +1.6 228,542 + +0.9 228,632 + GFF1046 0.19 +0.9 228,650 - GFF1046 0.22 +1.0 228,814 - GFF1046 0.45 +0.5 228,855 + GFF1046 0.50 +1.1 228,974 - GFF1046 0.67 +0.3 229,028 + GFF1046 0.74 +0.6 229,047 + GFF1046 0.77 +1.0 229,117 - GFF1046 0.87 +0.6 229,204 - -0.1
Or see this region's nucleotide sequence