Strain Fitness in Escherichia coli BW25113 around b2894

Experiment: toluene 2.5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecJ and dsbC are separated by 5 nucleotidesdsbC and xerD are separated by 24 nucleotidesxerD and fldB are separated by 111 nucleotidesfldB and ygfX are separated by 39 nucleotides b2892: recJ - ssDNA exonuclease, 5' --> 3'-specific (NCBI), at 3,034,395 to 3,036,128 recJ b2893: dsbC - protein disulfide isomerase II (NCBI), at 3,036,134 to 3,036,844 dsbC b2894: xerD - site-specific tyrosine recombinase XerD (NCBI), at 3,036,869 to 3,037,765 xerD b2895: fldB - flavodoxin 2 (NCBI), at 3,037,877 to 3,038,398 fldB b2896: ygfX - hypothetical protein (NCBI), at 3,038,438 to 3,038,845 ygfX Position (kb) 3036 3037 3038Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3035.869 kb on - strand, within recJat 3035.906 kb on - strand, within recJat 3035.906 kb on - strandat 3035.960 kb on - strandat 3035.996 kb on - strandat 3035.996 kb on - strandat 3035.996 kb on - strandat 3036.046 kb on - strandat 3036.068 kb on - strandat 3036.229 kb on + strand, within dsbCat 3036.229 kb on - strand, within dsbCat 3036.268 kb on + strand, within dsbCat 3036.457 kb on - strand, within dsbCat 3036.511 kb on + strand, within dsbCat 3036.511 kb on + strand, within dsbCat 3036.613 kb on - strand, within dsbCat 3036.626 kb on - strand, within dsbCat 3036.660 kb on - strand, within dsbCat 3036.660 kb on - strand, within dsbCat 3036.666 kb on + strand, within dsbCat 3036.668 kb on + strand, within dsbCat 3036.761 kb on + strand, within dsbCat 3037.372 kb on + strand, within xerDat 3037.773 kb on + strandat 3037.777 kb on + strandat 3037.777 kb on + strandat 3037.900 kb on + strandat 3037.900 kb on + strandat 3037.986 kb on - strand, within fldBat 3038.097 kb on + strand, within fldBat 3038.128 kb on + strand, within fldBat 3038.128 kb on + strand, within fldBat 3038.128 kb on + strand, within fldBat 3038.149 kb on + strand, within fldBat 3038.149 kb on + strand, within fldBat 3038.321 kb on + strand, within fldBat 3038.348 kb on - strandat 3038.348 kb on - strandat 3038.418 kb on - strandat 3038.516 kb on - strand, within ygfXat 3038.698 kb on - strand, within ygfXat 3038.714 kb on + strand, within ygfXat 3038.714 kb on + strand, within ygfXat 3038.716 kb on - strand, within ygfXat 3038.718 kb on + strand, within ygfXat 3038.718 kb on + strand, within ygfXat 3038.746 kb on - strand, within ygfX

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Per-strain Table

Position Strand Gene LocusTag Fraction toluene 2.5 vol%
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3,035,869 - recJ b2892 0.85 -1.2
3,035,906 - recJ b2892 0.87 -4.1
3,035,906 - +1.2
3,035,960 - -0.7
3,035,996 - -1.0
3,035,996 - -0.8
3,035,996 - +0.0
3,036,046 - -1.8
3,036,068 - -3.0
3,036,229 + dsbC b2893 0.13 +0.2
3,036,229 - dsbC b2893 0.13 -2.1
3,036,268 + dsbC b2893 0.19 +0.2
3,036,457 - dsbC b2893 0.45 -2.2
3,036,511 + dsbC b2893 0.53 +1.0
3,036,511 + dsbC b2893 0.53 +0.4
3,036,613 - dsbC b2893 0.67 -1.7
3,036,626 - dsbC b2893 0.69 -1.8
3,036,660 - dsbC b2893 0.74 -1.2
3,036,660 - dsbC b2893 0.74 -1.0
3,036,666 + dsbC b2893 0.75 -0.9
3,036,668 + dsbC b2893 0.75 -0.7
3,036,761 + dsbC b2893 0.88 +1.2
3,037,372 + xerD b2894 0.56 -3.2
3,037,773 + -2.9
3,037,777 + +1.5
3,037,777 + +2.0
3,037,900 + -2.2
3,037,900 + -2.6
3,037,986 - fldB b2895 0.21 -1.1
3,038,097 + fldB b2895 0.42 -2.2
3,038,128 + fldB b2895 0.48 -2.0
3,038,128 + fldB b2895 0.48 -2.7
3,038,128 + fldB b2895 0.48 +1.2
3,038,149 + fldB b2895 0.52 -1.7
3,038,149 + fldB b2895 0.52 -0.6
3,038,321 + fldB b2895 0.85 -2.2
3,038,348 - -3.8
3,038,348 - -0.5
3,038,418 - -0.5
3,038,516 - ygfX b2896 0.19 +0.8
3,038,698 - ygfX b2896 0.64 -0.8
3,038,714 + ygfX b2896 0.68 +1.0
3,038,714 + ygfX b2896 0.68 +0.6
3,038,716 - ygfX b2896 0.68 +0.7
3,038,718 + ygfX b2896 0.69 -1.8
3,038,718 + ygfX b2896 0.69 +1.2
3,038,746 - ygfX b2896 0.75 -0.4

Or see this region's nucleotide sequence