Strain Fitness in Escherichia coli BW25113 around b2535

Experiment: toluene 2.5 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	toluene 2.5 vol%
2,662,457	+				+0.4
2,662,590	-	yfhR	b2534	0.21	-1.3
2,662,595	-	yfhR	b2534	0.21	-1.3
2,662,595	-	yfhR	b2534	0.21	+0.9
2,662,650	+	yfhR	b2534	0.28	-0.1
2,662,788	-	yfhR	b2534	0.44	-2.6
2,662,823	-	yfhR	b2534	0.48	-1.1
2,662,857	+	yfhR	b2534	0.52	-1.4
2,662,870	-	yfhR	b2534	0.54	-2.2
2,662,905	+	yfhR	b2534	0.58	+0.3
2,662,991	+	yfhR	b2534	0.68	-0.0
2,662,999	-	yfhR	b2534	0.69	+0.3
2,663,113	-	yfhR	b2534	0.82	-0.1
2,663,120	+	yfhR	b2534	0.83	-0.5
2,663,157	+	yfhR	b2534	0.87	+0.5
2,663,304	+				+0.6
2,663,336	+				+0.1
2,663,393	+				+1.1
2,663,459	-				+0.3
2,663,469	-				+0.1
2,663,570	+				+1.1
2,663,570	+				+0.8
2,663,573	+				-0.6
2,663,578	-				+1.7
2,663,628	+	csiE	b2535	0.13	+1.0
2,663,628	+	csiE	b2535	0.13	+0.3
2,663,694	+	csiE	b2535	0.19	-0.2
2,663,697	-	csiE	b2535	0.19	-0.2
2,663,697	-	csiE	b2535	0.19	+1.2
2,663,733	-	csiE	b2535	0.22	+2.1
2,663,733	-	csiE	b2535	0.22	-0.6
2,663,745	-	csiE	b2535	0.22	-1.6
2,663,849	-	csiE	b2535	0.31	-1.3
2,663,927	+	csiE	b2535	0.37	+1.1
2,663,945	-	csiE	b2535	0.38	+0.7
2,663,945	-	csiE	b2535	0.38	+0.4
2,663,956	-	csiE	b2535	0.39	-0.4
2,663,956	-	csiE	b2535	0.39	-1.4
2,663,956	-	csiE	b2535	0.39	-1.3
2,663,981	+	csiE	b2535	0.41	-0.1
2,663,983	-	csiE	b2535	0.41	+1.0
2,664,044	+	csiE	b2535	0.46	+1.0
2,664,044	+	csiE	b2535	0.46	-0.8
2,664,058	+	csiE	b2535	0.47	+1.1
2,664,091	-	csiE	b2535	0.49	+1.4
2,664,091	-	csiE	b2535	0.49	+0.8
2,664,151	-	csiE	b2535	0.54	+0.4
2,664,151	-	csiE	b2535	0.54	-2.0
2,664,172	-	csiE	b2535	0.56	-0.7
2,664,242	+	csiE	b2535	0.61	+0.8
2,664,242	+	csiE	b2535	0.61	-0.5
2,664,242	+	csiE	b2535	0.61	-0.1
2,664,305	-	csiE	b2535	0.66	+0.8
2,664,315	-	csiE	b2535	0.67	-0.7
2,664,315	-	csiE	b2535	0.67	+0.3
2,664,317	+	csiE	b2535	0.67	-0.3
2,664,324	+	csiE	b2535	0.68	-0.7
2,664,326	+	csiE	b2535	0.68	+0.6
2,664,329	+	csiE	b2535	0.68	-0.0
2,664,329	+	csiE	b2535	0.68	+0.5
2,664,329	+	csiE	b2535	0.68	-3.2
2,664,349	+	csiE	b2535	0.70	+0.4
2,664,502	-	csiE	b2535	0.82	-0.3
2,664,535	-	csiE	b2535	0.84	-0.6
2,664,569	+	csiE	b2535	0.87	+0.3
2,664,629	+				+0.9
2,664,637	-				-1.3
2,664,637	-				-0.7
2,664,673	+				+0.1
2,664,681	-				-0.8
2,664,681	-				+0.7
2,664,687	+				-0.6
2,664,696	+				+0.3
2,664,732	+				+0.2
2,664,790	+				+0.4
2,664,890	+	hcaT	b2536	0.14	+0.4
2,664,933	-	hcaT	b2536	0.18	-1.6
2,665,029	+	hcaT	b2536	0.26	+0.1
2,665,232	+	hcaT	b2536	0.44	-2.9
2,665,232	+	hcaT	b2536	0.44	-0.6
2,665,242	+	hcaT	b2536	0.45	-0.9
2,665,242	+	hcaT	b2536	0.45	-1.9
2,665,318	-	hcaT	b2536	0.52	+0.4
2,665,358	+	hcaT	b2536	0.55	-0.7
2,665,358	+	hcaT	b2536	0.55	+1.5
2,665,375	+	hcaT	b2536	0.57	-0.0
2,665,375	+	hcaT	b2536	0.57	+0.0
2,665,383	-	hcaT	b2536	0.57	-0.3
2,665,518	-	hcaT	b2536	0.69	-0.4
2,665,518	-	hcaT	b2536	0.69	+1.2
2,665,530	-	hcaT	b2536	0.70	+0.7
2,665,530	-	hcaT	b2536	0.70	+1.7
2,665,556	+	hcaT	b2536	0.73	-0.7
2,665,556	+	hcaT	b2536	0.73	-0.8