Strain Fitness in Escherichia coli BW25113 around b2024

Experiment: toluene 2.5 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	toluene 2.5 vol%
2,092,160	+	hisB	b2022	0.63	-0.9
2,092,171	-	hisB	b2022	0.64	-0.2
2,092,193	-	hisB	b2022	0.66	-0.3
2,092,193	-	hisB	b2022	0.66	-3.0
2,092,355	+	hisB	b2022	0.81	+0.2
2,092,355	+	hisB	b2022	0.81	-0.7
2,092,359	-	hisB	b2022	0.81	+0.6
2,092,406	+	hisB	b2022	0.86	-0.1
2,092,406	+	hisB	b2022	0.86	+0.9
2,092,412	+	hisB	b2022	0.86	-0.5
2,092,412	+	hisB	b2022	0.86	-1.0
2,092,464	+				+0.3
2,092,533	-				-0.3
2,092,549	-				-2.4
2,092,752	-	hisH	b2023	0.33	+0.5
2,092,772	-	hisH	b2023	0.36	-0.0
2,092,907	+	hisH	b2023	0.59	+0.2
2,093,026	+	hisH	b2023	0.79	+0.8
2,093,026	+	hisH	b2023	0.79	+0.1
2,093,071	+	hisH	b2023	0.87	+1.6
2,093,071	+	hisH	b2023	0.87	-3.3
2,093,079	-	hisH	b2023	0.88	-0.4
2,093,079	-	hisH	b2023	0.88	-0.1
2,093,079	-	hisH	b2023	0.88	-0.3
2,093,079	-	hisH	b2023	0.88	-0.1
2,093,094	-				-0.4
2,093,183	+				+0.4
2,093,183	+				-0.2
2,093,454	-	hisA	b2024	0.41	+1.6
2,093,510	-	hisA	b2024	0.49	-0.1
2,093,818	-				-0.9
2,093,818	-				+1.0
2,093,881	+				-2.6
2,093,918	+				+0.9
2,093,978	+	hisF	b2025	0.14	+1.8
2,094,236	+	hisF	b2025	0.47	-0.3
2,094,236	+	hisF	b2025	0.47	-3.2
2,094,236	+	hisF	b2025	0.47	+0.5
2,094,244	-	hisF	b2025	0.48	+0.2
2,094,244	-	hisF	b2025	0.48	+0.7
2,094,248	-	hisF	b2025	0.49	-2.0
2,094,338	-	hisF	b2025	0.60	+1.0
2,094,596	+				-0.5
2,094,596	+				+0.2
2,094,647	+				-2.2
2,094,712	-	hisI	b2026	0.12	+0.7
2,094,712	-	hisI	b2026	0.12	+0.6
2,094,716	+	hisI	b2026	0.13	+1.0
2,094,869	+	hisI	b2026	0.38	-1.7