Experiment: toluene 2.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potG and potH are separated by 9 nucleotides potH and potI overlap by 4 nucleotides potI and ybjO are separated by 59 nucleotides ybjO and rumB are separated by 40 nucleotides
b0855: potG - ATP-binding component of putrescine transport system (VIMSS), at 894,214 to 895,347
potG
b0856: potH - putrescine transporter subunit: membrane component of ABC superfamily (NCBI), at 895,357 to 896,310
potH
b0857: potI - putrescine transporter subunit: membrane component of ABC superfamily (NCBI), at 896,307 to 897,152
potI
b0858: ybjO - predicted inner membrane protein (NCBI), at 897,212 to 897,700
ybjO
b0859: rumB - 23S rRNA m(5)U747-methyltransferase (NCBI), at 897,741 to 898,868
rumB
Position (kb) 896
897
898 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 895.325 kb on + strand at 895.325 kb on + strand at 895.461 kb on + strand, within potH at 895.461 kb on + strand, within potH at 895.603 kb on - strand, within potH at 895.694 kb on + strand, within potH at 895.848 kb on + strand, within potH at 895.861 kb on - strand, within potH at 895.930 kb on + strand, within potH at 895.937 kb on - strand, within potH at 895.937 kb on - strand, within potH at 896.095 kb on - strand, within potH at 896.425 kb on + strand, within potI at 896.425 kb on + strand, within potI at 896.429 kb on + strand, within potI at 896.429 kb on + strand, within potI at 896.497 kb on - strand, within potI at 896.497 kb on - strand, within potI at 896.607 kb on - strand, within potI at 896.607 kb on - strand, within potI at 897.041 kb on + strand, within potI at 897.053 kb on - strand, within potI at 897.084 kb on - strand at 897.084 kb on - strand at 897.371 kb on - strand, within ybjO at 897.402 kb on - strand, within ybjO at 897.426 kb on + strand, within ybjO at 897.702 kb on - strand at 897.724 kb on + strand at 897.744 kb on - strand at 897.780 kb on - strand at 897.815 kb on - strand at 897.815 kb on - strand at 897.894 kb on + strand, within rumB at 897.894 kb on + strand, within rumB at 897.929 kb on + strand, within rumB at 897.929 kb on + strand, within rumB at 897.953 kb on + strand, within rumB at 897.956 kb on - strand, within rumB at 897.960 kb on + strand, within rumB at 898.006 kb on - strand, within rumB at 898.006 kb on - strand, within rumB
Per-strain Table
Position Strand Gene LocusTag Fraction toluene 2.5 vol% remove 895,325 + -2.0 895,325 + +1.8 895,461 + potH b0856 0.11 -1.1 895,461 + potH b0856 0.11 +0.9 895,603 - potH b0856 0.26 +1.0 895,694 + potH b0856 0.35 -1.6 895,848 + potH b0856 0.51 +0.4 895,861 - potH b0856 0.53 -0.6 895,930 + potH b0856 0.60 +0.8 895,937 - potH b0856 0.61 -0.2 895,937 - potH b0856 0.61 -1.0 896,095 - potH b0856 0.77 -0.3 896,425 + potI b0857 0.14 -1.6 896,425 + potI b0857 0.14 -3.0 896,429 + potI b0857 0.14 -0.2 896,429 + potI b0857 0.14 -1.5 896,497 - potI b0857 0.22 -1.6 896,497 - potI b0857 0.22 -2.0 896,607 - potI b0857 0.35 -0.6 896,607 - potI b0857 0.35 +0.3 897,041 + potI b0857 0.87 -0.4 897,053 - potI b0857 0.88 -2.1 897,084 - -0.3 897,084 - -0.1 897,371 - ybjO b0858 0.33 -1.4 897,402 - ybjO b0858 0.39 +1.8 897,426 + ybjO b0858 0.44 -3.1 897,702 - -2.6 897,724 + +0.6 897,744 - +1.4 897,780 - -1.9 897,815 - -1.3 897,815 - -1.6 897,894 + rumB b0859 0.14 -0.4 897,894 + rumB b0859 0.14 +1.7 897,929 + rumB b0859 0.17 -2.1 897,929 + rumB b0859 0.17 -1.0 897,953 + rumB b0859 0.19 +0.1 897,956 - rumB b0859 0.19 -0.4 897,960 + rumB b0859 0.19 +0.2 898,006 - rumB b0859 0.23 -1.6 898,006 - rumB b0859 0.23 +0.8
Or see this region's nucleotide sequence