Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF947 and GFF948 overlap by 1 nucleotides GFF948 and GFF949 are separated by 136 nucleotides GFF949 and GFF950 are separated by 487 nucleotides
GFF947 - Valyl-tRNA synthetase (EC 6.1.1.9), at 120,365 to 123,220
GFF947
GFF948 - DNA polymerase III chi subunit (EC 2.7.7.7), at 123,220 to 123,663
GFF948
GFF949 - Cytosol aminopeptidase PepA (EC 3.4.11.1), at 123,800 to 125,311
GFF949
GFF950 - FIG000988: Predicted permease, at 125,799 to 126,806
GFF950
Position (kb)
123
124
125
126 Strain fitness (log2 ratio)
-1
0
1 at 123.363 kb on + strand, within GFF948 at 123.364 kb on - strand, within GFF948 at 123.364 kb on - strand, within GFF948 at 123.364 kb on - strand, within GFF948 at 123.644 kb on + strand at 123.665 kb on + strand at 123.666 kb on - strand at 123.689 kb on - strand at 123.854 kb on + strand at 123.930 kb on + strand at 123.930 kb on + strand at 123.930 kb on + strand at 123.934 kb on + strand at 123.940 kb on + strand at 124.218 kb on + strand, within GFF949 at 124.218 kb on + strand, within GFF949 at 124.552 kb on - strand, within GFF949 at 124.552 kb on - strand, within GFF949 at 124.672 kb on - strand, within GFF949 at 124.672 kb on - strand, within GFF949 at 124.708 kb on - strand, within GFF949 at 124.717 kb on + strand, within GFF949 at 124.847 kb on - strand, within GFF949 at 124.897 kb on + strand, within GFF949 at 124.900 kb on - strand, within GFF949 at 124.900 kb on - strand, within GFF949 at 125.021 kb on + strand, within GFF949 at 125.044 kb on + strand, within GFF949 at 125.045 kb on - strand, within GFF949 at 125.045 kb on - strand, within GFF949 at 125.046 kb on + strand, within GFF949 at 125.047 kb on - strand, within GFF949 at 125.187 kb on + strand at 125.188 kb on - strand at 125.357 kb on - strand at 125.415 kb on + strand at 125.475 kb on + strand at 125.546 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 123,363 + GFF948 0.32 +0.2 123,364 - GFF948 0.32 -1.2 123,364 - GFF948 0.32 -0.4 123,364 - GFF948 0.32 -0.9 123,644 + +0.2 123,665 + -1.0 123,666 - -0.1 123,689 - +0.3 123,854 + +1.1 123,930 + +0.5 123,930 + +1.2 123,930 + +0.4 123,934 + -0.4 123,940 + +0.6 124,218 + GFF949 0.28 +0.6 124,218 + GFF949 0.28 +0.9 124,552 - GFF949 0.50 -0.7 124,552 - GFF949 0.50 +0.1 124,672 - GFF949 0.58 +0.4 124,672 - GFF949 0.58 -0.9 124,708 - GFF949 0.60 +0.6 124,717 + GFF949 0.61 +0.9 124,847 - GFF949 0.69 +0.9 124,897 + GFF949 0.73 -0.2 124,900 - GFF949 0.73 +0.5 124,900 - GFF949 0.73 +0.5 125,021 + GFF949 0.81 -0.4 125,044 + GFF949 0.82 +0.7 125,045 - GFF949 0.82 +0.6 125,045 - GFF949 0.82 +0.9 125,046 + GFF949 0.82 +0.9 125,047 - GFF949 0.82 +0.8 125,187 + +0.6 125,188 - +1.0 125,357 - -0.0 125,415 + +0.9 125,475 + +0.1 125,546 + +0.6
Or see this region's nucleotide sequence