Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF910 and GFF911 are separated by 11 nucleotides GFF911 and GFF912 are separated by 58 nucleotides GFF912 and GFF913 overlap by 17 nucleotides
GFF910 - Putative inner membrane protein, at 88,329 to 89,105
GFF910
GFF911 - Putative inner membrane protein, at 89,117 to 89,761
GFF911
GFF912 - Metallo-dependent hydrolases, subgroup B, at 89,820 to 90,953
GFF912
GFF913 - D-Glucosaminate-6-phosphate ammonia-lyase (EC 4.3.1.-), at 90,937 to 92,055
GFF913
Position (kb)
89
90
91 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 88.928 kb on + strand, within GFF910 at 89.207 kb on - strand, within GFF911 at 89.210 kb on + strand, within GFF911 at 89.211 kb on - strand, within GFF911 at 89.213 kb on + strand, within GFF911 at 89.213 kb on + strand, within GFF911 at 89.213 kb on + strand, within GFF911 at 89.213 kb on + strand, within GFF911 at 89.213 kb on + strand, within GFF911 at 89.213 kb on + strand, within GFF911 at 89.214 kb on - strand, within GFF911 at 89.214 kb on - strand, within GFF911 at 89.219 kb on + strand, within GFF911 at 89.237 kb on - strand, within GFF911 at 89.498 kb on + strand, within GFF911 at 89.518 kb on + strand, within GFF911 at 89.678 kb on + strand, within GFF911 at 89.678 kb on + strand, within GFF911 at 89.679 kb on - strand, within GFF911 at 89.679 kb on - strand, within GFF911 at 89.679 kb on - strand, within GFF911 at 89.759 kb on + strand at 89.759 kb on + strand at 89.830 kb on + strand at 89.830 kb on + strand at 89.870 kb on + strand at 89.871 kb on - strand at 89.873 kb on + strand at 89.874 kb on - strand at 90.096 kb on - strand, within GFF912 at 90.142 kb on - strand, within GFF912 at 90.173 kb on + strand, within GFF912 at 90.174 kb on - strand, within GFF912 at 90.254 kb on + strand, within GFF912 at 90.367 kb on + strand, within GFF912 at 90.367 kb on + strand, within GFF912 at 90.368 kb on - strand, within GFF912 at 90.368 kb on - strand, within GFF912 at 90.371 kb on + strand, within GFF912 at 90.371 kb on + strand, within GFF912 at 90.372 kb on - strand, within GFF912 at 90.372 kb on - strand, within GFF912 at 90.372 kb on - strand, within GFF912 at 90.585 kb on - strand, within GFF912 at 90.605 kb on + strand, within GFF912 at 90.605 kb on + strand, within GFF912 at 90.606 kb on - strand, within GFF912 at 90.625 kb on - strand, within GFF912 at 90.625 kb on - strand, within GFF912 at 90.665 kb on + strand, within GFF912 at 90.806 kb on + strand, within GFF912 at 90.807 kb on - strand, within GFF912 at 90.813 kb on - strand, within GFF912 at 90.856 kb on + strand at 90.856 kb on + strand at 90.857 kb on - strand at 90.909 kb on + strand at 91.103 kb on - strand, within GFF913 at 91.227 kb on + strand, within GFF913 at 91.228 kb on - strand, within GFF913 at 91.381 kb on - strand, within GFF913 at 91.405 kb on + strand, within GFF913 at 91.405 kb on + strand, within GFF913 at 91.405 kb on + strand, within GFF913 at 91.408 kb on - strand, within GFF913 at 91.427 kb on + strand, within GFF913 at 91.480 kb on - strand, within GFF913 at 91.554 kb on + strand, within GFF913 at 91.558 kb on + strand, within GFF913 at 91.632 kb on - strand, within GFF913 at 91.752 kb on - strand, within GFF913 at 91.811 kb on + strand, within GFF913 at 91.921 kb on + strand, within GFF913 at 91.930 kb on + strand, within GFF913 at 91.931 kb on - strand, within GFF913
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 88,928 + GFF910 0.77 -0.5 89,207 - GFF911 0.14 -0.1 89,210 + GFF911 0.14 +0.1 89,211 - GFF911 0.15 +2.3 89,213 + GFF911 0.15 -1.6 89,213 + GFF911 0.15 -0.5 89,213 + GFF911 0.15 +0.0 89,213 + GFF911 0.15 -1.2 89,213 + GFF911 0.15 +0.2 89,213 + GFF911 0.15 +0.9 89,214 - GFF911 0.15 +0.2 89,214 - GFF911 0.15 +0.4 89,219 + GFF911 0.16 -1.4 89,237 - GFF911 0.19 +0.3 89,498 + GFF911 0.59 +0.3 89,518 + GFF911 0.62 +0.3 89,678 + GFF911 0.87 +0.1 89,678 + GFF911 0.87 -0.0 89,679 - GFF911 0.87 +0.1 89,679 - GFF911 0.87 +0.9 89,679 - GFF911 0.87 -0.5 89,759 + +1.5 89,759 + +0.1 89,830 + -0.2 89,830 + +0.6 89,870 + -0.1 89,871 - -0.1 89,873 + -0.1 89,874 - -0.7 90,096 - GFF912 0.24 -0.1 90,142 - GFF912 0.28 +0.3 90,173 + GFF912 0.31 +0.7 90,174 - GFF912 0.31 -0.1 90,254 + GFF912 0.38 +0.6 90,367 + GFF912 0.48 +0.2 90,367 + GFF912 0.48 +0.1 90,368 - GFF912 0.48 +0.2 90,368 - GFF912 0.48 -0.0 90,371 + GFF912 0.49 -1.2 90,371 + GFF912 0.49 -1.0 90,372 - GFF912 0.49 +0.4 90,372 - GFF912 0.49 -0.4 90,372 - GFF912 0.49 +0.0 90,585 - GFF912 0.67 +1.2 90,605 + GFF912 0.69 -1.2 90,605 + GFF912 0.69 -0.5 90,606 - GFF912 0.69 -0.0 90,625 - GFF912 0.71 +0.3 90,625 - GFF912 0.71 -1.1 90,665 + GFF912 0.75 +0.1 90,806 + GFF912 0.87 -1.1 90,807 - GFF912 0.87 +0.8 90,813 - GFF912 0.88 -0.3 90,856 + +0.9 90,856 + -0.0 90,857 - -2.1 90,909 + -0.3 91,103 - GFF913 0.15 +0.6 91,227 + GFF913 0.26 -0.0 91,228 - GFF913 0.26 -0.7 91,381 - GFF913 0.40 -0.5 91,405 + GFF913 0.42 +0.1 91,405 + GFF913 0.42 +0.1 91,405 + GFF913 0.42 +0.0 91,408 - GFF913 0.42 +0.2 91,427 + GFF913 0.44 +0.1 91,480 - GFF913 0.49 -0.6 91,554 + GFF913 0.55 -1.8 91,558 + GFF913 0.55 +0.1 91,632 - GFF913 0.62 -1.4 91,752 - GFF913 0.73 +0.4 91,811 + GFF913 0.78 +0.3 91,921 + GFF913 0.88 -0.3 91,930 + GFF913 0.89 +0.1 91,931 - GFF913 0.89 -1.2
Or see this region's nucleotide sequence