Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF9

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntGFF8 and GFF9 are separated by 227 nucleotidesGFF9 and GFF10 are separated by 309 nucleotides GFF8 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase I alpha (EC 2.5.1.54), at 4,506 to 5,558 GFF8 GFF9 - Fumarate hydratase class I, aerobic (EC 4.2.1.2); L(+)-tartrate dehydratase beta subunit (EC 4.2.1.32), at 5,786 to 5,902 GFF9 GFF10 - L(+)-tartrate dehydratase alpha subunit (EC 4.2.1.32), at 6,212 to 7,057 GFF10 Position (kb) 5 6Strain fitness (log2 ratio) -2 -1 0 1at 5.226 kb on + strand, within GFF8at 5.226 kb on + strand, within GFF8at 5.422 kb on + strand, within GFF8at 5.600 kb on + strandat 5.643 kb on - strandat 5.666 kb on + strandat 5.670 kb on + strandat 5.691 kb on - strandat 5.966 kb on + strandat 6.244 kb on + strandat 6.358 kb on + strand, within GFF10at 6.809 kb on + strand, within GFF10at 6.810 kb on - strand, within GFF10

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
remove
5,226 + GFF8 0.68 +0.5
5,226 + GFF8 0.68 +0.6
5,422 + GFF8 0.87 +0.2
5,600 + -0.0
5,643 - -1.9
5,666 + +0.2
5,670 + +0.9
5,691 - -0.1
5,966 + -2.0
6,244 + +0.0
6,358 + GFF10 0.17 -0.4
6,809 + GFF10 0.71 +0.1
6,810 - GFF10 0.71 +0.2

Or see this region's nucleotide sequence