Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF82

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF81 and GFF82 are separated by 91 nucleotidesGFF82 and GFF83 are separated by 138 nucleotides GFF81 - Ferric hydroxamate outer membrane receptor FhuA, at 63,162 to 65,273 GFF81 GFF82 - hypothetical protein, at 65,365 to 65,505 GFF82 GFF83 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 65,644 to 68,166 GFF83 Position (kb) 65 66Strain fitness (log2 ratio) -1 0 1 2at 64.403 kb on - strand, within GFF81at 64.429 kb on + strand, within GFF81at 64.552 kb on + strand, within GFF81at 64.566 kb on - strand, within GFF81at 64.782 kb on - strand, within GFF81at 64.997 kb on + strand, within GFF81at 65.026 kb on - strand, within GFF81at 65.132 kb on + strandat 65.133 kb on - strandat 65.296 kb on - strandat 65.355 kb on + strandat 65.356 kb on - strandat 65.357 kb on + strandat 65.396 kb on - strand, within GFF82at 65.528 kb on + strandat 65.572 kb on + strandat 65.809 kb on - strandat 65.874 kb on - strandat 65.917 kb on + strand, within GFF83at 65.982 kb on - strand, within GFF83at 66.179 kb on + strand, within GFF83at 66.228 kb on - strand, within GFF83at 66.238 kb on - strand, within GFF83at 66.350 kb on - strand, within GFF83

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
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64,403 - GFF81 0.59 +0.4
64,429 + GFF81 0.60 +0.1
64,552 + GFF81 0.66 +0.0
64,566 - GFF81 0.66 +0.2
64,782 - GFF81 0.77 -0.1
64,997 + GFF81 0.87 -0.1
65,026 - GFF81 0.88 +0.1
65,132 + +0.9
65,133 - +0.5
65,296 - +0.2
65,355 + +0.1
65,356 - +0.2
65,357 + -0.8
65,396 - GFF82 0.22 +0.7
65,528 + -0.5
65,572 + +0.2
65,809 - +1.2
65,874 - +1.1
65,917 + GFF83 0.11 +1.9
65,982 - GFF83 0.13 +2.0
66,179 + GFF83 0.21 +2.0
66,228 - GFF83 0.23 +1.9
66,238 - GFF83 0.24 +2.3
66,350 - GFF83 0.28 -0.2

Or see this region's nucleotide sequence