Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF754 and GFF755 overlap by 17 nucleotides GFF755 and GFF756 are separated by 57 nucleotides GFF756 and GFF757 are separated by 96 nucleotides GFF757 and GFF758 are separated by 345 nucleotides
GFF754 - YbjC protein, clustered with oxygen-insensitive NADPH nitroreductase, at 112,571 to 112,861
GFF754
GFF755 - Oxygen-insensitive NADPH nitroreductase (EC 1.-.-.-), at 112,845 to 113,567
GFF755
GFF756 - Ribosomal protein S6 glutaminyl transferase, at 113,625 to 114,527
GFF756
GFF757 - putative sensory transduction regulator, at 114,624 to 115,100
GFF757
GFF758 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 115,446 to 116,561
GFF758
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-2
-1
0
1 at 112.670 kb on - strand, within GFF754 at 112.707 kb on + strand, within GFF754 at 112.707 kb on + strand, within GFF754 at 112.934 kb on + strand, within GFF755 at 112.986 kb on - strand, within GFF755 at 113.227 kb on - strand, within GFF755 at 113.291 kb on - strand, within GFF755 at 113.439 kb on + strand, within GFF755 at 113.443 kb on - strand, within GFF755 at 113.443 kb on - strand, within GFF755 at 113.460 kb on - strand, within GFF755 at 113.536 kb on - strand at 113.536 kb on - strand at 113.566 kb on - strand at 113.584 kb on + strand at 113.717 kb on - strand, within GFF756 at 113.717 kb on - strand, within GFF756 at 113.737 kb on - strand, within GFF756 at 113.739 kb on - strand, within GFF756 at 113.748 kb on - strand, within GFF756 at 113.772 kb on + strand, within GFF756 at 113.832 kb on - strand, within GFF756 at 113.839 kb on - strand, within GFF756 at 113.839 kb on - strand, within GFF756 at 113.907 kb on - strand, within GFF756 at 114.015 kb on - strand, within GFF756 at 114.159 kb on + strand, within GFF756 at 114.159 kb on + strand, within GFF756 at 114.160 kb on - strand, within GFF756 at 114.173 kb on + strand, within GFF756 at 114.613 kb on - strand at 114.623 kb on + strand at 114.653 kb on + strand at 114.654 kb on - strand at 114.753 kb on + strand, within GFF757 at 114.937 kb on + strand, within GFF757 at 115.098 kb on + strand at 115.296 kb on + strand at 115.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 112,670 - GFF754 0.34 -0.5 112,707 + GFF754 0.47 +0.3 112,707 + GFF754 0.47 -0.3 112,934 + GFF755 0.12 +0.6 112,986 - GFF755 0.20 -1.3 113,227 - GFF755 0.53 +0.2 113,291 - GFF755 0.62 +0.8 113,439 + GFF755 0.82 +0.4 113,443 - GFF755 0.83 +0.4 113,443 - GFF755 0.83 -0.9 113,460 - GFF755 0.85 +0.7 113,536 - +0.4 113,536 - -0.9 113,566 - +0.1 113,584 + +0.7 113,717 - GFF756 0.10 -0.2 113,717 - GFF756 0.10 -1.9 113,737 - GFF756 0.12 +0.9 113,739 - GFF756 0.13 -0.5 113,748 - GFF756 0.14 -0.5 113,772 + GFF756 0.16 -0.7 113,832 - GFF756 0.23 -0.2 113,839 - GFF756 0.24 -1.3 113,839 - GFF756 0.24 -0.8 113,907 - GFF756 0.31 -0.2 114,015 - GFF756 0.43 -0.5 114,159 + GFF756 0.59 +1.2 114,159 + GFF756 0.59 -0.1 114,160 - GFF756 0.59 -0.9 114,173 + GFF756 0.61 +0.7 114,613 - -0.5 114,623 + -0.5 114,653 + +0.0 114,654 - -1.0 114,753 + GFF757 0.27 -0.7 114,937 + GFF757 0.66 -0.6 115,098 + +0.0 115,296 + +0.6 115,375 - +0.6
Or see this region's nucleotide sequence