Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF752 and GFF753 are separated by 31 nucleotides GFF753 and GFF754 are separated by 169 nucleotides GFF754 and GFF755 overlap by 17 nucleotides GFF755 and GFF756 are separated by 57 nucleotides
GFF752 - Inner membrane protein, at 111,804 to 112,106
GFF752
GFF753 - Glutaredoxin 1, at 112,138 to 112,401
GFF753
GFF754 - YbjC protein, clustered with oxygen-insensitive NADPH nitroreductase, at 112,571 to 112,861
GFF754
GFF755 - Oxygen-insensitive NADPH nitroreductase (EC 1.-.-.-), at 112,845 to 113,567
GFF755
GFF756 - Ribosomal protein S6 glutaminyl transferase, at 113,625 to 114,527
GFF756
Position (kb)
112
113
114 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 112.015 kb on + strand, within GFF752 at 112.234 kb on + strand, within GFF753 at 112.296 kb on + strand, within GFF753 at 112.365 kb on - strand, within GFF753 at 112.395 kb on + strand at 112.396 kb on - strand at 112.401 kb on + strand at 112.401 kb on + strand at 112.401 kb on + strand at 112.441 kb on + strand at 112.442 kb on - strand at 112.505 kb on + strand at 112.507 kb on + strand at 112.507 kb on + strand at 112.539 kb on - strand at 112.670 kb on - strand, within GFF754 at 112.707 kb on + strand, within GFF754 at 112.707 kb on + strand, within GFF754 at 112.934 kb on + strand, within GFF755 at 112.986 kb on - strand, within GFF755 at 113.227 kb on - strand, within GFF755 at 113.291 kb on - strand, within GFF755 at 113.439 kb on + strand, within GFF755 at 113.443 kb on - strand, within GFF755 at 113.443 kb on - strand, within GFF755 at 113.460 kb on - strand, within GFF755 at 113.536 kb on - strand at 113.536 kb on - strand at 113.566 kb on - strand at 113.584 kb on + strand at 113.717 kb on - strand, within GFF756 at 113.717 kb on - strand, within GFF756 at 113.737 kb on - strand, within GFF756 at 113.739 kb on - strand, within GFF756 at 113.748 kb on - strand, within GFF756 at 113.772 kb on + strand, within GFF756 at 113.832 kb on - strand, within GFF756 at 113.839 kb on - strand, within GFF756 at 113.839 kb on - strand, within GFF756 at 113.907 kb on - strand, within GFF756 at 114.015 kb on - strand, within GFF756 at 114.159 kb on + strand, within GFF756 at 114.159 kb on + strand, within GFF756 at 114.160 kb on - strand, within GFF756 at 114.173 kb on + strand, within GFF756
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 112,015 + GFF752 0.70 -0.1 112,234 + GFF753 0.36 +1.0 112,296 + GFF753 0.60 +1.0 112,365 - GFF753 0.86 +0.7 112,395 + +0.4 112,396 - +1.1 112,401 + -0.6 112,401 + +0.0 112,401 + +0.3 112,441 + +0.5 112,442 - +2.8 112,505 + -0.7 112,507 + -2.4 112,507 + -0.1 112,539 - -0.1 112,670 - GFF754 0.34 -0.5 112,707 + GFF754 0.47 +0.3 112,707 + GFF754 0.47 -0.3 112,934 + GFF755 0.12 +0.6 112,986 - GFF755 0.20 -1.3 113,227 - GFF755 0.53 +0.2 113,291 - GFF755 0.62 +0.8 113,439 + GFF755 0.82 +0.4 113,443 - GFF755 0.83 +0.4 113,443 - GFF755 0.83 -0.9 113,460 - GFF755 0.85 +0.7 113,536 - +0.4 113,536 - -0.9 113,566 - +0.1 113,584 + +0.7 113,717 - GFF756 0.10 -0.2 113,717 - GFF756 0.10 -1.9 113,737 - GFF756 0.12 +0.9 113,739 - GFF756 0.13 -0.5 113,748 - GFF756 0.14 -0.5 113,772 + GFF756 0.16 -0.7 113,832 - GFF756 0.23 -0.2 113,839 - GFF756 0.24 -1.3 113,839 - GFF756 0.24 -0.8 113,907 - GFF756 0.31 -0.2 114,015 - GFF756 0.43 -0.5 114,159 + GFF756 0.59 +1.2 114,159 + GFF756 0.59 -0.1 114,160 - GFF756 0.59 -0.9 114,173 + GFF756 0.61 +0.7
Or see this region's nucleotide sequence