Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF71 and GFF72 overlap by 4 nucleotides GFF72 and GFF73 are separated by 18 nucleotides GFF73 and GFF74 are separated by 17 nucleotides
GFF71 - Minor fimbrial subunit StfF, at 53,355 to 53,831
GFF71
GFF72 - Minor fimbrial subunit StfE, at 53,828 to 54,340
GFF72
GFF73 - Periplasmic fimbrial chaperone StfD, at 54,359 to 55,111
GFF73
GFF74 - Fimbriae usher protein StfC, at 55,129 to 57,786
GFF74
Position (kb) 54
55
56 Strain fitness (log2 ratio) -2
-1
0
1 at 53.490 kb on - strand, within GFF71 at 53.730 kb on - strand, within GFF71 at 53.835 kb on + strand at 54.257 kb on - strand, within GFF72 at 54.406 kb on + strand at 54.407 kb on - strand at 54.450 kb on + strand, within GFF73 at 54.589 kb on - strand, within GFF73 at 54.589 kb on - strand, within GFF73 at 54.645 kb on + strand, within GFF73 at 54.649 kb on - strand, within GFF73 at 54.649 kb on - strand, within GFF73 at 54.890 kb on + strand, within GFF73 at 54.945 kb on + strand, within GFF73 at 54.992 kb on - strand, within GFF73 at 55.191 kb on + strand at 55.191 kb on + strand at 55.192 kb on - strand at 55.205 kb on - strand at 55.326 kb on + strand at 55.397 kb on - strand, within GFF74 at 55.427 kb on + strand, within GFF74 at 55.620 kb on - strand, within GFF74 at 55.620 kb on - strand, within GFF74 at 55.620 kb on - strand, within GFF74 at 55.620 kb on - strand, within GFF74 at 55.662 kb on + strand, within GFF74 at 55.662 kb on + strand, within GFF74 at 55.663 kb on - strand, within GFF74 at 55.663 kb on - strand, within GFF74 at 55.663 kb on - strand, within GFF74 at 55.815 kb on + strand, within GFF74 at 55.859 kb on + strand, within GFF74 at 55.860 kb on - strand, within GFF74 at 55.878 kb on - strand, within GFF74 at 55.881 kb on - strand, within GFF74 at 55.955 kb on + strand, within GFF74 at 56.035 kb on - strand, within GFF74 at 56.048 kb on + strand, within GFF74 at 56.048 kb on + strand, within GFF74 at 56.076 kb on - strand, within GFF74 at 56.076 kb on - strand, within GFF74
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 53,490 - GFF71 0.28 +0.1 53,730 - GFF71 0.79 -0.2 53,835 + -0.1 54,257 - GFF72 0.84 -0.2 54,406 + +0.0 54,407 - -0.7 54,450 + GFF73 0.12 +0.2 54,589 - GFF73 0.31 -0.3 54,589 - GFF73 0.31 +0.1 54,645 + GFF73 0.38 -0.9 54,649 - GFF73 0.39 +0.3 54,649 - GFF73 0.39 +1.1 54,890 + GFF73 0.71 +1.0 54,945 + GFF73 0.78 +0.1 54,992 - GFF73 0.84 +0.3 55,191 + -2.0 55,191 + -0.4 55,192 - -0.2 55,205 - -0.2 55,326 + -0.3 55,397 - GFF74 0.10 -1.4 55,427 + GFF74 0.11 +0.4 55,620 - GFF74 0.18 -0.7 55,620 - GFF74 0.18 +0.6 55,620 - GFF74 0.18 +0.6 55,620 - GFF74 0.18 +0.6 55,662 + GFF74 0.20 +0.0 55,662 + GFF74 0.20 -0.7 55,663 - GFF74 0.20 +0.4 55,663 - GFF74 0.20 -0.9 55,663 - GFF74 0.20 -1.5 55,815 + GFF74 0.26 -0.6 55,859 + GFF74 0.27 -1.3 55,860 - GFF74 0.28 +1.1 55,878 - GFF74 0.28 +0.5 55,881 - GFF74 0.28 -0.2 55,955 + GFF74 0.31 +0.5 56,035 - GFF74 0.34 -0.2 56,048 + GFF74 0.35 +0.7 56,048 + GFF74 0.35 +0.4 56,076 - GFF74 0.36 +0.9 56,076 - GFF74 0.36 -0.7
Or see this region's nucleotide sequence