Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF716 and GFF717 are separated by 218 nucleotides GFF717 and GFF718 are separated by 455 nucleotides
GFF716 - L,D-transpeptidase YbiS, at 70,961 to 71,881
GFF716
GFF717 - Putative ATPase component of ABC transporter with duplicated ATPase domain, at 72,100 to 73,692
GFF717
GFF718 - putative inner membrane protein, at 74,148 to 75,044
GFF718
Position (kb) 72
73
74 Strain fitness (log2 ratio) -2
-1
0
1 at 71.136 kb on + strand, within GFF716 at 71.250 kb on - strand, within GFF716 at 71.310 kb on + strand, within GFF716 at 71.311 kb on - strand, within GFF716 at 71.488 kb on + strand, within GFF716 at 71.547 kb on + strand, within GFF716 at 71.550 kb on + strand, within GFF716 at 71.551 kb on - strand, within GFF716 at 71.734 kb on + strand, within GFF716 at 71.911 kb on - strand at 71.926 kb on - strand at 71.948 kb on + strand at 72.062 kb on - strand at 72.065 kb on - strand at 72.093 kb on + strand at 72.138 kb on + strand at 72.407 kb on - strand, within GFF717 at 72.442 kb on - strand, within GFF717 at 72.461 kb on + strand, within GFF717 at 72.462 kb on - strand, within GFF717 at 72.585 kb on - strand, within GFF717 at 72.608 kb on + strand, within GFF717 at 72.615 kb on + strand, within GFF717 at 72.667 kb on - strand, within GFF717 at 72.669 kb on + strand, within GFF717 at 72.756 kb on + strand, within GFF717 at 72.808 kb on + strand, within GFF717 at 72.808 kb on + strand, within GFF717 at 72.809 kb on - strand, within GFF717 at 72.809 kb on - strand, within GFF717 at 72.809 kb on - strand, within GFF717 at 72.809 kb on - strand, within GFF717 at 72.817 kb on + strand, within GFF717 at 72.818 kb on - strand, within GFF717 at 72.818 kb on - strand, within GFF717 at 72.818 kb on - strand, within GFF717 at 73.258 kb on + strand, within GFF717 at 73.276 kb on + strand, within GFF717 at 73.276 kb on + strand, within GFF717 at 73.359 kb on + strand, within GFF717 at 73.359 kb on + strand, within GFF717 at 73.377 kb on - strand, within GFF717 at 73.377 kb on - strand, within GFF717 at 73.392 kb on + strand, within GFF717 at 73.690 kb on + strand at 73.777 kb on + strand at 73.781 kb on + strand at 74.679 kb on - strand, within GFF718
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 71,136 + GFF716 0.19 -1.8 71,250 - GFF716 0.31 -2.4 71,310 + GFF716 0.38 -0.5 71,311 - GFF716 0.38 +0.2 71,488 + GFF716 0.57 -1.8 71,547 + GFF716 0.64 -2.0 71,550 + GFF716 0.64 -1.4 71,551 - GFF716 0.64 -1.3 71,734 + GFF716 0.84 -1.2 71,911 - -1.6 71,926 - -0.3 71,948 + -1.5 72,062 - +0.7 72,065 - +0.3 72,093 + +0.3 72,138 + +1.3 72,407 - GFF717 0.19 +0.9 72,442 - GFF717 0.21 -0.7 72,461 + GFF717 0.23 +1.1 72,462 - GFF717 0.23 +0.9 72,585 - GFF717 0.30 +1.0 72,608 + GFF717 0.32 +0.7 72,615 + GFF717 0.32 +0.4 72,667 - GFF717 0.36 -0.6 72,669 + GFF717 0.36 +1.3 72,756 + GFF717 0.41 +1.2 72,808 + GFF717 0.44 +0.9 72,808 + GFF717 0.44 +0.8 72,809 - GFF717 0.45 +0.9 72,809 - GFF717 0.45 +0.5 72,809 - GFF717 0.45 +0.2 72,809 - GFF717 0.45 +0.9 72,817 + GFF717 0.45 +0.5 72,818 - GFF717 0.45 +0.6 72,818 - GFF717 0.45 +1.7 72,818 - GFF717 0.45 +1.3 73,258 + GFF717 0.73 +0.5 73,276 + GFF717 0.74 +1.0 73,276 + GFF717 0.74 +0.4 73,359 + GFF717 0.79 +0.7 73,359 + GFF717 0.79 +0.5 73,377 - GFF717 0.80 +0.4 73,377 - GFF717 0.80 +0.6 73,392 + GFF717 0.81 +0.7 73,690 + +0.2 73,777 + +0.8 73,781 + +0.0 74,679 - GFF718 0.59 -0.4
Or see this region's nucleotide sequence