Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF677 and GFF678 are separated by 396 nucleotides GFF678 and GFF679 are separated by 21 nucleotides GFF679 and GFF680 are separated by 2 nucleotides
GFF677 - Putative structural protein, at 36,397 to 37,305
GFF677
GFF678 - Molybdenum cofactor biosynthesis protein MoaA, at 37,702 to 38,691
GFF678
GFF679 - Molybdenum cofactor biosynthesis protein MoaB, at 38,713 to 39,225
GFF679
GFF680 - Molybdenum cofactor biosynthesis protein MoaC, at 39,228 to 39,713
GFF680
Position (kb) 37
38
39 Strain fitness (log2 ratio) -1
0
1
2 at 37.185 kb on + strand, within GFF677 at 37.213 kb on + strand, within GFF677 at 37.214 kb on - strand, within GFF677 at 37.214 kb on - strand, within GFF677 at 37.244 kb on - strand at 37.368 kb on - strand at 37.368 kb on - strand at 37.390 kb on + strand at 37.445 kb on - strand at 37.445 kb on - strand at 37.447 kb on - strand at 37.447 kb on - strand at 37.551 kb on + strand at 37.552 kb on - strand at 37.570 kb on - strand at 37.588 kb on - strand at 37.640 kb on + strand at 37.699 kb on + strand at 37.699 kb on + strand at 37.700 kb on - strand at 37.742 kb on - strand at 37.742 kb on - strand at 37.744 kb on + strand at 37.773 kb on + strand at 37.929 kb on - strand, within GFF678 at 37.978 kb on - strand, within GFF678 at 38.322 kb on + strand, within GFF678 at 38.337 kb on - strand, within GFF678 at 38.519 kb on + strand, within GFF678 at 38.581 kb on + strand, within GFF678 at 38.581 kb on + strand, within GFF678 at 38.581 kb on + strand, within GFF678 at 38.582 kb on - strand, within GFF678 at 38.659 kb on - strand at 38.678 kb on - strand at 39.247 kb on + strand at 39.510 kb on - strand, within GFF680 at 39.635 kb on + strand, within GFF680 at 39.640 kb on + strand, within GFF680 at 39.641 kb on - strand, within GFF680
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 37,185 + GFF677 0.87 +0.4 37,213 + GFF677 0.90 +1.0 37,214 - GFF677 0.90 -0.2 37,214 - GFF677 0.90 +0.4 37,244 - +0.1 37,368 - +0.9 37,368 - -0.5 37,390 + +0.7 37,445 - +1.2 37,445 - -0.1 37,447 - -0.1 37,447 - +0.2 37,551 + +0.1 37,552 - +0.7 37,570 - -0.1 37,588 - +0.9 37,640 + +0.8 37,699 + -0.1 37,699 + +1.0 37,700 - +1.7 37,742 - +1.4 37,742 - +1.4 37,744 + +1.4 37,773 + +1.0 37,929 - GFF678 0.23 -0.0 37,978 - GFF678 0.28 +1.3 38,322 + GFF678 0.63 +1.9 38,337 - GFF678 0.64 +1.7 38,519 + GFF678 0.83 +1.4 38,581 + GFF678 0.89 +1.4 38,581 + GFF678 0.89 +1.6 38,581 + GFF678 0.89 +1.1 38,582 - GFF678 0.89 +0.9 38,659 - +0.5 38,678 - +1.2 39,247 + +1.1 39,510 - GFF680 0.58 -1.0 39,635 + GFF680 0.84 +1.1 39,640 + GFF680 0.85 +0.9 39,641 - GFF680 0.85 +1.5
Or see this region's nucleotide sequence