Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF632 and GFF633 are separated by 17 nucleotides GFF633 and GFF634 overlap by 8 nucleotides GFF634 and GFF635 overlap by 8 nucleotides
GFF632 - Methyl-directed repair DNA adenine methylase (EC 2.1.1.72), at 373,556 to 374,392
GFF632
GFF633 - Ribulose-phosphate 3-epimerase (EC 5.1.3.1), at 374,410 to 375,087
GFF633
GFF634 - Phosphoglycolate phosphatase (EC 3.1.3.18), at 375,080 to 375,838
GFF634
GFF635 - Tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 375,831 to 376,835
GFF635
Position (kb) 375
376 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 374.305 kb on + strand, within GFF632 at 374.419 kb on + strand at 374.420 kb on - strand at 374.533 kb on + strand, within GFF633 at 374.534 kb on - strand, within GFF633 at 374.718 kb on - strand, within GFF633 at 374.718 kb on - strand, within GFF633 at 374.742 kb on + strand, within GFF633 at 374.783 kb on - strand, within GFF633 at 374.791 kb on + strand, within GFF633 at 375.085 kb on + strand at 375.331 kb on + strand, within GFF634 at 375.331 kb on + strand, within GFF634 at 375.331 kb on + strand, within GFF634 at 375.332 kb on - strand, within GFF634 at 375.352 kb on - strand, within GFF634 at 375.355 kb on + strand, within GFF634 at 375.356 kb on - strand, within GFF634 at 375.380 kb on + strand, within GFF634 at 375.380 kb on + strand, within GFF634 at 375.468 kb on + strand, within GFF634 at 375.537 kb on - strand, within GFF634 at 375.574 kb on + strand, within GFF634 at 375.713 kb on + strand, within GFF634 at 375.714 kb on - strand, within GFF634 at 375.719 kb on + strand, within GFF634 at 375.725 kb on + strand, within GFF634 at 375.764 kb on + strand at 375.776 kb on - strand at 375.836 kb on + strand at 375.891 kb on + strand at 375.891 kb on + strand at 375.946 kb on - strand, within GFF635 at 375.968 kb on + strand, within GFF635 at 375.998 kb on - strand, within GFF635 at 376.002 kb on - strand, within GFF635 at 376.123 kb on - strand, within GFF635 at 376.124 kb on + strand, within GFF635 at 376.124 kb on + strand, within GFF635 at 376.129 kb on - strand, within GFF635 at 376.167 kb on - strand, within GFF635 at 376.533 kb on + strand, within GFF635
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 374,305 + GFF632 0.89 +1.2 374,419 + +0.5 374,420 - -0.3 374,533 + GFF633 0.18 -4.2 374,534 - GFF633 0.18 -1.8 374,718 - GFF633 0.45 -1.5 374,718 - GFF633 0.45 +0.7 374,742 + GFF633 0.49 -1.6 374,783 - GFF633 0.55 -0.4 374,791 + GFF633 0.56 -1.4 375,085 + -0.1 375,331 + GFF634 0.33 +0.2 375,331 + GFF634 0.33 +0.7 375,331 + GFF634 0.33 -0.1 375,332 - GFF634 0.33 +2.4 375,352 - GFF634 0.36 +1.0 375,355 + GFF634 0.36 +0.2 375,356 - GFF634 0.36 +2.3 375,380 + GFF634 0.40 +0.4 375,380 + GFF634 0.40 +1.6 375,468 + GFF634 0.51 -0.1 375,537 - GFF634 0.60 +1.7 375,574 + GFF634 0.65 +0.3 375,713 + GFF634 0.83 -0.4 375,714 - GFF634 0.84 +3.1 375,719 + GFF634 0.84 -0.3 375,725 + GFF634 0.85 +0.0 375,764 + +0.6 375,776 - +3.4 375,836 + +1.9 375,891 + +1.4 375,891 + +2.7 375,946 - GFF635 0.11 +2.8 375,968 + GFF635 0.14 +2.4 375,998 - GFF635 0.17 +2.7 376,002 - GFF635 0.17 +1.1 376,123 - GFF635 0.29 +2.4 376,124 + GFF635 0.29 +2.6 376,124 + GFF635 0.29 +2.0 376,129 - GFF635 0.30 +2.4 376,167 - GFF635 0.33 +3.0 376,533 + GFF635 0.70 +2.2
Or see this region's nucleotide sequence