Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF576 and GFF577 are separated by 14 nucleotides GFF577 and GFF578 overlap by 1 nucleotides
GFF576 - Glycogen debranching enzyme (EC 3.2.1.-), at 306,980 to 308,956
GFF576
GFF577 - Glucose-1-phosphate adenylyltransferase (EC 2.7.7.27), at 308,971 to 310,266
GFF577
GFF578 - Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21), at 310,266 to 311,699
GFF578
Position (kb)
308
309
310
311 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 308.396 kb on + strand, within GFF576 at 308.396 kb on + strand, within GFF576 at 308.443 kb on - strand, within GFF576 at 308.443 kb on - strand, within GFF576 at 308.557 kb on + strand, within GFF576 at 308.671 kb on - strand, within GFF576 at 308.680 kb on + strand, within GFF576 at 308.681 kb on - strand, within GFF576 at 308.767 kb on + strand at 308.767 kb on + strand at 308.768 kb on - strand at 308.836 kb on + strand at 308.836 kb on + strand at 308.836 kb on + strand at 308.836 kb on + strand at 308.839 kb on - strand at 308.982 kb on - strand at 309.000 kb on - strand at 309.038 kb on + strand at 309.039 kb on - strand at 309.144 kb on - strand, within GFF577 at 309.193 kb on + strand, within GFF577 at 309.193 kb on + strand, within GFF577 at 309.220 kb on - strand, within GFF577 at 309.291 kb on + strand, within GFF577 at 309.351 kb on + strand, within GFF577 at 309.351 kb on + strand, within GFF577 at 309.352 kb on - strand, within GFF577 at 309.374 kb on - strand, within GFF577 at 309.400 kb on - strand, within GFF577 at 309.400 kb on - strand, within GFF577 at 309.403 kb on + strand, within GFF577 at 309.404 kb on - strand, within GFF577 at 309.424 kb on - strand, within GFF577 at 309.424 kb on - strand, within GFF577 at 309.472 kb on - strand, within GFF577 at 309.640 kb on + strand, within GFF577 at 309.641 kb on - strand, within GFF577 at 309.800 kb on + strand, within GFF577 at 309.800 kb on + strand, within GFF577 at 309.801 kb on - strand, within GFF577 at 309.801 kb on - strand, within GFF577 at 309.804 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.868 kb on + strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.869 kb on - strand, within GFF577 at 309.891 kb on + strand, within GFF577 at 309.892 kb on - strand, within GFF577 at 309.898 kb on - strand, within GFF577 at 309.951 kb on + strand, within GFF577 at 309.988 kb on - strand, within GFF577 at 309.988 kb on - strand, within GFF577 at 310.038 kb on - strand, within GFF577 at 310.053 kb on + strand, within GFF577 at 310.107 kb on - strand, within GFF577 at 310.134 kb on + strand, within GFF577 at 310.135 kb on - strand, within GFF577 at 310.209 kb on + strand at 310.209 kb on + strand at 310.276 kb on + strand at 310.276 kb on + strand at 310.282 kb on + strand at 310.283 kb on - strand at 310.461 kb on - strand, within GFF578 at 310.485 kb on + strand, within GFF578 at 310.485 kb on + strand, within GFF578 at 310.486 kb on - strand, within GFF578 at 310.486 kb on - strand, within GFF578 at 310.565 kb on - strand, within GFF578 at 310.565 kb on - strand, within GFF578 at 310.566 kb on + strand, within GFF578 at 310.572 kb on + strand, within GFF578 at 310.573 kb on - strand, within GFF578 at 310.603 kb on + strand, within GFF578 at 310.758 kb on + strand, within GFF578 at 310.758 kb on + strand, within GFF578 at 310.817 kb on + strand, within GFF578 at 310.873 kb on - strand, within GFF578 at 310.875 kb on + strand, within GFF578 at 310.886 kb on + strand, within GFF578 at 310.886 kb on + strand, within GFF578 at 310.887 kb on - strand, within GFF578 at 310.888 kb on + strand, within GFF578 at 310.909 kb on - strand, within GFF578 at 310.909 kb on - strand, within GFF578 at 310.923 kb on - strand, within GFF578 at 310.996 kb on + strand, within GFF578
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 308,396 + GFF576 0.72 -0.1 308,396 + GFF576 0.72 +1.4 308,443 - GFF576 0.74 +0.1 308,443 - GFF576 0.74 +0.7 308,557 + GFF576 0.80 +0.1 308,671 - GFF576 0.86 +0.5 308,680 + GFF576 0.86 +0.5 308,681 - GFF576 0.86 +0.3 308,767 + -0.5 308,767 + -0.4 308,768 - +0.1 308,836 + +0.0 308,836 + -0.1 308,836 + -0.6 308,836 + +0.1 308,839 - +0.5 308,982 - -0.0 309,000 - +0.2 309,038 + +0.4 309,039 - +0.7 309,144 - GFF577 0.13 +0.8 309,193 + GFF577 0.17 -0.1 309,193 + GFF577 0.17 +0.4 309,220 - GFF577 0.19 -0.0 309,291 + GFF577 0.25 -1.3 309,351 + GFF577 0.29 +0.8 309,351 + GFF577 0.29 +0.4 309,352 - GFF577 0.29 -0.4 309,374 - GFF577 0.31 +1.0 309,400 - GFF577 0.33 -2.0 309,400 - GFF577 0.33 +0.8 309,403 + GFF577 0.33 -0.0 309,404 - GFF577 0.33 +0.5 309,424 - GFF577 0.35 +1.5 309,424 - GFF577 0.35 -0.9 309,472 - GFF577 0.39 +0.5 309,640 + GFF577 0.52 +1.0 309,641 - GFF577 0.52 +0.4 309,800 + GFF577 0.64 +0.3 309,800 + GFF577 0.64 +1.4 309,801 - GFF577 0.64 +0.5 309,801 - GFF577 0.64 -1.0 309,804 + GFF577 0.64 +0.3 309,868 + GFF577 0.69 +0.6 309,868 + GFF577 0.69 +0.4 309,868 + GFF577 0.69 +0.2 309,868 + GFF577 0.69 +0.3 309,869 - GFF577 0.69 -0.4 309,869 - GFF577 0.69 -0.0 309,869 - GFF577 0.69 +0.1 309,869 - GFF577 0.69 +0.5 309,891 + GFF577 0.71 +0.3 309,892 - GFF577 0.71 +0.2 309,898 - GFF577 0.72 +0.4 309,951 + GFF577 0.76 -1.3 309,988 - GFF577 0.78 -0.5 309,988 - GFF577 0.78 +0.3 310,038 - GFF577 0.82 -1.4 310,053 + GFF577 0.83 -0.9 310,107 - GFF577 0.88 +1.2 310,134 + GFF577 0.90 +0.5 310,135 - GFF577 0.90 +1.0 310,209 + +0.6 310,209 + +0.6 310,276 + +0.1 310,276 + +1.6 310,282 + +1.5 310,283 - +1.5 310,461 - GFF578 0.14 +1.4 310,485 + GFF578 0.15 +1.8 310,485 + GFF578 0.15 +1.5 310,486 - GFF578 0.15 +1.6 310,486 - GFF578 0.15 +1.7 310,565 - GFF578 0.21 +1.7 310,565 - GFF578 0.21 +2.2 310,566 + GFF578 0.21 +0.6 310,572 + GFF578 0.21 +1.7 310,573 - GFF578 0.21 +1.0 310,603 + GFF578 0.24 +2.0 310,758 + GFF578 0.34 +1.9 310,758 + GFF578 0.34 +2.0 310,817 + GFF578 0.38 +2.5 310,873 - GFF578 0.42 +1.3 310,875 + GFF578 0.42 +1.0 310,886 + GFF578 0.43 +1.6 310,886 + GFF578 0.43 +2.3 310,887 - GFF578 0.43 +1.9 310,888 + GFF578 0.43 +2.6 310,909 - GFF578 0.45 +1.9 310,909 - GFF578 0.45 +1.7 310,923 - GFF578 0.46 +1.7 310,996 + GFF578 0.51 +1.5
Or see this region's nucleotide sequence