Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF51

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF49 and GFF50 are separated by 53 nucleotidesGFF50 and GFF51 are separated by 146 nucleotidesGFF51 and GFF52 are separated by 144 nucleotides GFF49 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase (EC 1.1.1.267), at 30,047 to 31,330 GFF49 GFF50 - Ribosome recycling factor, at 31,384 to 31,941 GFF50 GFF51 - Uridine monophosphate kinase (EC 2.7.4.22), at 32,088 to 32,813 GFF51 GFF52 - Translation elongation factor Ts, at 32,958 to 33,809 GFF52 Position (kb) 32 33Strain fitness (log2 ratio) -1 0 1 2at 31.327 kb on - strandat 31.365 kb on - strandat 31.375 kb on - strandat 31.992 kb on - strandat 32.005 kb on - strandat 32.038 kb on - strandat 32.868 kb on - strandat 32.970 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
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31,327 - -0.4
31,365 - -0.7
31,375 - -0.5
31,992 - -0.2
32,005 - +0.2
32,038 - +0.3
32,868 - -0.0
32,970 - +2.3

Or see this region's nucleotide sequence