Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF491

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF490 and GFF491 are separated by 10 nucleotidesGFF491 and GFF492 are separated by 3 nucleotides GFF490 - Cellulose synthase catalytic subunit [UDP-forming] (EC 2.4.1.12), at 209,612 to 212,236 GFF490 GFF491 - Cyclic di-GMP binding protein precursor, at 212,247 to 214,547 GFF491 GFF492 - Endoglucanase precursor (EC 3.2.1.4), at 214,551 to 215,660 GFF492 Position (kb) 212 213 214 215Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 211.269 kb on - strand, within GFF490at 211.269 kb on - strand, within GFF490at 211.303 kb on - strand, within GFF490at 211.339 kb on + strand, within GFF490at 211.362 kb on - strand, within GFF490at 211.362 kb on - strand, within GFF490at 211.409 kb on + strand, within GFF490at 211.410 kb on - strand, within GFF490at 211.410 kb on - strand, within GFF490at 211.500 kb on - strand, within GFF490at 211.529 kb on - strand, within GFF490at 211.583 kb on + strand, within GFF490at 211.584 kb on - strand, within GFF490at 211.584 kb on - strand, within GFF490at 211.640 kb on + strand, within GFF490at 211.640 kb on + strand, within GFF490at 211.641 kb on - strand, within GFF490at 211.641 kb on - strand, within GFF490at 211.688 kb on - strand, within GFF490at 211.773 kb on + strand, within GFF490at 211.877 kb on + strand, within GFF490at 211.958 kb on - strand, within GFF490at 211.976 kb on - strandat 212.014 kb on + strandat 212.030 kb on - strandat 212.112 kb on + strandat 212.196 kb on + strandat 212.241 kb on - strandat 212.291 kb on - strandat 212.462 kb on + strandat 212.557 kb on - strand, within GFF491at 212.677 kb on + strand, within GFF491at 212.677 kb on + strand, within GFF491at 212.677 kb on + strand, within GFF491at 212.678 kb on - strand, within GFF491at 212.681 kb on + strand, within GFF491at 212.682 kb on - strand, within GFF491at 212.696 kb on + strand, within GFF491at 212.798 kb on + strand, within GFF491at 212.817 kb on + strand, within GFF491at 212.817 kb on + strand, within GFF491at 212.817 kb on + strand, within GFF491at 212.818 kb on - strand, within GFF491at 212.818 kb on - strand, within GFF491at 213.012 kb on + strand, within GFF491at 213.013 kb on - strand, within GFF491at 213.013 kb on - strand, within GFF491at 213.139 kb on - strand, within GFF491at 213.174 kb on - strand, within GFF491at 213.174 kb on - strand, within GFF491at 213.216 kb on - strand, within GFF491at 213.216 kb on - strand, within GFF491at 213.254 kb on - strand, within GFF491at 213.414 kb on + strand, within GFF491at 213.440 kb on + strand, within GFF491at 213.454 kb on - strand, within GFF491at 213.462 kb on - strand, within GFF491at 213.530 kb on + strand, within GFF491at 213.652 kb on + strand, within GFF491at 213.653 kb on - strand, within GFF491at 213.653 kb on - strand, within GFF491at 213.653 kb on - strand, within GFF491at 213.669 kb on + strand, within GFF491at 213.671 kb on + strand, within GFF491at 213.672 kb on - strand, within GFF491at 213.707 kb on + strand, within GFF491at 213.707 kb on + strand, within GFF491at 213.708 kb on - strand, within GFF491at 213.708 kb on - strand, within GFF491at 213.711 kb on - strand, within GFF491at 213.775 kb on - strand, within GFF491at 214.127 kb on + strand, within GFF491at 214.277 kb on - strand, within GFF491at 214.380 kb on + strandat 214.380 kb on + strandat 214.380 kb on + strandat 214.380 kb on + strandat 214.384 kb on - strandat 214.477 kb on + strandat 214.477 kb on + strandat 214.508 kb on + strandat 214.559 kb on + strandat 214.603 kb on + strandat 214.622 kb on + strandat 214.623 kb on - strandat 214.706 kb on + strand, within GFF492at 214.729 kb on - strand, within GFF492at 214.828 kb on - strand, within GFF492at 215.076 kb on - strand, within GFF492at 215.097 kb on + strand, within GFF492at 215.098 kb on - strand, within GFF492at 215.224 kb on - strand, within GFF492at 215.278 kb on - strand, within GFF492at 215.295 kb on + strand, within GFF492at 215.345 kb on - strand, within GFF492at 215.547 kb on + strand, within GFF492at 215.547 kb on + strand, within GFF492

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
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211,269 - GFF490 0.63 +0.4
211,269 - GFF490 0.63 -0.2
211,303 - GFF490 0.64 +0.5
211,339 + GFF490 0.66 -0.2
211,362 - GFF490 0.67 +0.5
211,362 - GFF490 0.67 -0.0
211,409 + GFF490 0.68 -0.1
211,410 - GFF490 0.68 -1.3
211,410 - GFF490 0.68 -1.5
211,500 - GFF490 0.72 +0.2
211,529 - GFF490 0.73 +0.0
211,583 + GFF490 0.75 -0.0
211,584 - GFF490 0.75 -1.1
211,584 - GFF490 0.75 -0.1
211,640 + GFF490 0.77 +1.2
211,640 + GFF490 0.77 -0.1
211,641 - GFF490 0.77 -0.1
211,641 - GFF490 0.77 +0.5
211,688 - GFF490 0.79 +0.4
211,773 + GFF490 0.82 +0.6
211,877 + GFF490 0.86 +0.3
211,958 - GFF490 0.89 -0.1
211,976 - -0.3
212,014 + -0.7
212,030 - +1.7
212,112 + +0.2
212,196 + +0.7
212,241 - -0.4
212,291 - -1.0
212,462 + -0.1
212,557 - GFF491 0.13 -0.1
212,677 + GFF491 0.19 -1.0
212,677 + GFF491 0.19 -0.2
212,677 + GFF491 0.19 -0.3
212,678 - GFF491 0.19 -1.2
212,681 + GFF491 0.19 -0.2
212,682 - GFF491 0.19 +0.1
212,696 + GFF491 0.20 -0.3
212,798 + GFF491 0.24 -0.3
212,817 + GFF491 0.25 +0.3
212,817 + GFF491 0.25 +0.4
212,817 + GFF491 0.25 -1.4
212,818 - GFF491 0.25 -0.0
212,818 - GFF491 0.25 -0.4
213,012 + GFF491 0.33 +0.3
213,013 - GFF491 0.33 -1.6
213,013 - GFF491 0.33 +0.2
213,139 - GFF491 0.39 -3.2
213,174 - GFF491 0.40 -0.3
213,174 - GFF491 0.40 -0.2
213,216 - GFF491 0.42 -1.7
213,216 - GFF491 0.42 -0.9
213,254 - GFF491 0.44 -1.5
213,414 + GFF491 0.51 +1.5
213,440 + GFF491 0.52 -0.4
213,454 - GFF491 0.52 +0.2
213,462 - GFF491 0.53 -1.4
213,530 + GFF491 0.56 -0.2
213,652 + GFF491 0.61 +0.1
213,653 - GFF491 0.61 +0.2
213,653 - GFF491 0.61 -0.1
213,653 - GFF491 0.61 -0.3
213,669 + GFF491 0.62 -0.2
213,671 + GFF491 0.62 -0.2
213,672 - GFF491 0.62 +0.1
213,707 + GFF491 0.63 -1.0
213,707 + GFF491 0.63 -0.4
213,708 - GFF491 0.63 -0.3
213,708 - GFF491 0.63 +0.1
213,711 - GFF491 0.64 -0.2
213,775 - GFF491 0.66 -0.4
214,127 + GFF491 0.82 +1.1
214,277 - GFF491 0.88 +0.2
214,380 + -0.1
214,380 + +0.5
214,380 + -0.1
214,380 + +0.2
214,384 - -0.1
214,477 + -0.1
214,477 + -1.7
214,508 + -0.3
214,559 + +0.6
214,603 + +0.9
214,622 + +0.4
214,623 - +0.3
214,706 + GFF492 0.14 -1.2
214,729 - GFF492 0.16 +1.0
214,828 - GFF492 0.25 +1.0
215,076 - GFF492 0.47 +0.6
215,097 + GFF492 0.49 +1.3
215,098 - GFF492 0.49 +1.2
215,224 - GFF492 0.61 +2.6
215,278 - GFF492 0.65 -1.6
215,295 + GFF492 0.67 +1.2
215,345 - GFF492 0.72 +3.2
215,547 + GFF492 0.90 +0.7
215,547 + GFF492 0.90 +0.9

Or see this region's nucleotide sequence