Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4908 and GFF4909 are separated by 143 nucleotides GFF4909 and GFF4910 are separated by 8 nucleotides
GFF4908 - FIG003145: Surface protein, at 9,418 to 10,317
GFF4908
GFF4909 - Hydroxylamine reductase (EC 1.7.-.-), at 10,461 to 12,113
GFF4909
GFF4910 - NADH oxidoreductase hcr (EC 1.-.-.-), at 12,122 to 13,093
GFF4910
Position (kb)
10
11
12
13 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 9.513 kb on - strand, within GFF4908 at 9.535 kb on - strand, within GFF4908 at 9.625 kb on - strand, within GFF4908 at 9.635 kb on + strand, within GFF4908 at 9.720 kb on + strand, within GFF4908 at 9.720 kb on + strand, within GFF4908 at 9.721 kb on - strand, within GFF4908 at 9.831 kb on + strand, within GFF4908 at 10.129 kb on - strand, within GFF4908 at 10.217 kb on + strand, within GFF4908 at 10.259 kb on + strand at 10.259 kb on + strand at 10.259 kb on + strand at 10.260 kb on - strand at 10.379 kb on - strand at 10.538 kb on + strand at 10.634 kb on + strand, within GFF4909 at 10.691 kb on + strand, within GFF4909 at 10.866 kb on - strand, within GFF4909 at 10.953 kb on + strand, within GFF4909 at 11.008 kb on - strand, within GFF4909 at 11.136 kb on + strand, within GFF4909 at 11.223 kb on + strand, within GFF4909 at 11.337 kb on + strand, within GFF4909 at 11.447 kb on + strand, within GFF4909 at 11.448 kb on - strand, within GFF4909 at 11.662 kb on + strand, within GFF4909 at 11.696 kb on + strand, within GFF4909 at 11.700 kb on + strand, within GFF4909 at 11.701 kb on - strand, within GFF4909 at 11.762 kb on + strand, within GFF4909 at 11.834 kb on + strand, within GFF4909 at 11.844 kb on + strand, within GFF4909 at 11.845 kb on - strand, within GFF4909 at 12.224 kb on + strand, within GFF4910 at 12.225 kb on - strand, within GFF4910 at 12.328 kb on + strand, within GFF4910 at 12.328 kb on + strand, within GFF4910 at 12.329 kb on - strand, within GFF4910 at 12.329 kb on - strand, within GFF4910 at 12.329 kb on - strand, within GFF4910 at 12.329 kb on - strand, within GFF4910 at 12.404 kb on + strand, within GFF4910 at 12.741 kb on - strand, within GFF4910 at 12.919 kb on + strand, within GFF4910 at 12.919 kb on + strand, within GFF4910 at 12.920 kb on - strand, within GFF4910 at 13.043 kb on + strand at 13.048 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 9,513 - GFF4908 0.11 -0.4 9,535 - GFF4908 0.13 +0.0 9,625 - GFF4908 0.23 +0.2 9,635 + GFF4908 0.24 +0.8 9,720 + GFF4908 0.34 +0.1 9,720 + GFF4908 0.34 +0.4 9,721 - GFF4908 0.34 -0.7 9,831 + GFF4908 0.46 -0.4 10,129 - GFF4908 0.79 +0.1 10,217 + GFF4908 0.89 +0.5 10,259 + +0.6 10,259 + +0.6 10,259 + +1.7 10,260 - -0.3 10,379 - +0.1 10,538 + +0.8 10,634 + GFF4909 0.10 +0.2 10,691 + GFF4909 0.14 -0.2 10,866 - GFF4909 0.25 +0.1 10,953 + GFF4909 0.30 +0.4 11,008 - GFF4909 0.33 +0.9 11,136 + GFF4909 0.41 +0.5 11,223 + GFF4909 0.46 +0.3 11,337 + GFF4909 0.53 +0.3 11,447 + GFF4909 0.60 -1.0 11,448 - GFF4909 0.60 +0.1 11,662 + GFF4909 0.73 -0.0 11,696 + GFF4909 0.75 -1.0 11,700 + GFF4909 0.75 +0.4 11,701 - GFF4909 0.75 -0.2 11,762 + GFF4909 0.79 +0.0 11,834 + GFF4909 0.83 +1.3 11,844 + GFF4909 0.84 +0.3 11,845 - GFF4909 0.84 +0.9 12,224 + GFF4910 0.10 +0.0 12,225 - GFF4910 0.11 -0.1 12,328 + GFF4910 0.21 +0.7 12,328 + GFF4910 0.21 -0.9 12,329 - GFF4910 0.21 -3.1 12,329 - GFF4910 0.21 -1.4 12,329 - GFF4910 0.21 -0.4 12,329 - GFF4910 0.21 -0.3 12,404 + GFF4910 0.29 -0.8 12,741 - GFF4910 0.64 -0.2 12,919 + GFF4910 0.82 -2.5 12,919 + GFF4910 0.82 -0.5 12,920 - GFF4910 0.82 -0.9 13,043 + +0.4 13,048 - +0.2
Or see this region's nucleotide sequence