Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF479 and GFF480 are separated by 100 nucleotides GFF480 and GFF481 are separated by 117 nucleotides GFF481 and GFF482 overlap by 4 nucleotides GFF482 and GFF483 are separated by 103 nucleotides GFF483 and GFF484 are separated by 81 nucleotides
GFF479 - Dipeptide transport ATP-binding protein DppF (TC 3.A.1.5.2), at 201,735 to 202,748
GFF479
GFF480 - Serine transporter, at 202,849 to 204,147
GFF480
GFF481 - hypothetical protein, at 204,265 to 204,405
GFF481
GFF482 - FIG01047459: hypothetical protein, at 204,402 to 204,662
GFF482
GFF483 - hypothetical protein, at 204,766 to 204,897
GFF483
GFF484 - FIG002337: predicted inner membrane protein, at 204,979 to 206,658
GFF484
Position (kb)
202
203
204
205 Strain fitness (log2 ratio)
-2
-1
0
1 at 201.973 kb on - strand, within GFF479 at 201.976 kb on - strand, within GFF479 at 201.996 kb on - strand, within GFF479 at 202.122 kb on - strand, within GFF479 at 202.377 kb on + strand, within GFF479 at 202.377 kb on + strand, within GFF479 at 202.378 kb on - strand, within GFF479 at 202.378 kb on - strand, within GFF479 at 202.385 kb on + strand, within GFF479 at 202.385 kb on + strand, within GFF479 at 202.440 kb on + strand, within GFF479 at 202.440 kb on + strand, within GFF479 at 202.441 kb on - strand, within GFF479 at 202.444 kb on + strand, within GFF479 at 202.503 kb on + strand, within GFF479 at 202.652 kb on + strand at 202.674 kb on - strand at 202.790 kb on - strand at 202.895 kb on + strand at 202.974 kb on + strand at 203.005 kb on + strand, within GFF480 at 203.229 kb on + strand, within GFF480 at 203.230 kb on - strand, within GFF480 at 203.254 kb on + strand, within GFF480 at 203.374 kb on - strand, within GFF480 at 203.375 kb on + strand, within GFF480 at 203.376 kb on - strand, within GFF480 at 203.824 kb on + strand, within GFF480 at 204.011 kb on - strand, within GFF480 at 204.147 kb on + strand at 204.184 kb on - strand at 204.197 kb on + strand at 204.198 kb on - strand at 204.210 kb on + strand at 204.211 kb on - strand at 204.398 kb on + strand at 204.398 kb on + strand at 204.399 kb on - strand at 204.464 kb on - strand, within GFF482 at 204.512 kb on + strand, within GFF482 at 204.663 kb on - strand at 204.701 kb on + strand at 204.704 kb on - strand at 204.789 kb on + strand, within GFF483 at 204.789 kb on + strand, within GFF483 at 204.798 kb on + strand, within GFF483 at 205.022 kb on + strand at 205.022 kb on + strand at 205.022 kb on + strand at 205.030 kb on - strand at 205.057 kb on - strand at 205.138 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 201,973 - GFF479 0.23 -0.1 201,976 - GFF479 0.24 +0.3 201,996 - GFF479 0.26 -1.1 202,122 - GFF479 0.38 -1.2 202,377 + GFF479 0.63 -0.0 202,377 + GFF479 0.63 -0.1 202,378 - GFF479 0.63 +0.1 202,378 - GFF479 0.63 -0.0 202,385 + GFF479 0.64 +0.2 202,385 + GFF479 0.64 +0.3 202,440 + GFF479 0.70 -0.4 202,440 + GFF479 0.70 +0.5 202,441 - GFF479 0.70 -0.8 202,444 + GFF479 0.70 -1.8 202,503 + GFF479 0.76 +0.2 202,652 + +0.1 202,674 - -0.3 202,790 - +1.3 202,895 + +1.0 202,974 + -0.1 203,005 + GFF480 0.12 -0.2 203,229 + GFF480 0.29 +0.3 203,230 - GFF480 0.29 +0.3 203,254 + GFF480 0.31 +0.0 203,374 - GFF480 0.40 +0.3 203,375 + GFF480 0.40 +0.1 203,376 - GFF480 0.41 +0.5 203,824 + GFF480 0.75 +0.3 204,011 - GFF480 0.89 +0.4 204,147 + +1.0 204,184 - +0.3 204,197 + -0.8 204,198 - +0.2 204,210 + -0.1 204,211 - +0.4 204,398 + +0.2 204,398 + +0.1 204,399 - -1.4 204,464 - GFF482 0.24 +0.3 204,512 + GFF482 0.42 -0.0 204,663 - +0.9 204,701 + -0.2 204,704 - -0.1 204,789 + GFF483 0.17 +0.2 204,789 + GFF483 0.17 -0.3 204,798 + GFF483 0.24 +0.4 205,022 + -1.3 205,022 + -0.1 205,022 + -0.2 205,030 - +0.3 205,057 - +0.4 205,138 - -1.3
Or see this region's nucleotide sequence