Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4784 and GFF4785 are separated by 170 nucleotides GFF4785 and GFF4786 overlap by 1 nucleotides GFF4786 and GFF4787 are separated by 10 nucleotides GFF4787 and GFF4788 are separated by 44 nucleotides
GFF4784 - Transcriptional regulator, MarR family, at 92,390 to 92,794
GFF4784
GFF4785 - 3-polyprenyl-4-hydroxybenzoate carboxy-lyase UbiX (EC 4.1.1.-), at 92,965 to 93,558
GFF4785
GFF4786 - UbiD family decarboxylase, MJ1133 type, at 93,558 to 94,985
GFF4786
GFF4787 - Hydroxyaromatic non-oxidative decarboxylase protein D (EC 4.1.1.-), at 94,996 to 95,232
GFF4787
GFF4788 - RNA polymerase sigma factor RpoS, at 95,277 to 96,269
GFF4788
Position (kb) 93
94
95 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 92.787 kb on - strand at 92.827 kb on + strand at 92.850 kb on - strand at 92.942 kb on - strand at 93.143 kb on - strand, within GFF4785 at 93.143 kb on - strand, within GFF4785 at 93.210 kb on + strand, within GFF4785 at 93.210 kb on + strand, within GFF4785 at 93.211 kb on - strand, within GFF4785 at 93.211 kb on - strand, within GFF4785 at 93.211 kb on - strand, within GFF4785 at 93.225 kb on + strand, within GFF4785 at 93.225 kb on + strand, within GFF4785 at 93.226 kb on - strand, within GFF4785 at 93.444 kb on + strand, within GFF4785 at 93.495 kb on + strand, within GFF4785 at 93.495 kb on + strand, within GFF4785 at 93.729 kb on - strand, within GFF4786 at 93.729 kb on - strand, within GFF4786 at 93.753 kb on - strand, within GFF4786 at 93.899 kb on + strand, within GFF4786 at 93.975 kb on + strand, within GFF4786 at 93.976 kb on - strand, within GFF4786 at 94.051 kb on - strand, within GFF4786 at 94.076 kb on + strand, within GFF4786 at 94.076 kb on + strand, within GFF4786 at 94.191 kb on - strand, within GFF4786 at 94.271 kb on + strand, within GFF4786 at 94.350 kb on - strand, within GFF4786 at 94.584 kb on + strand, within GFF4786 at 94.584 kb on + strand, within GFF4786 at 94.585 kb on - strand, within GFF4786 at 94.585 kb on - strand, within GFF4786 at 94.795 kb on + strand, within GFF4786 at 94.795 kb on + strand, within GFF4786 at 94.905 kb on + strand at 94.906 kb on - strand at 94.980 kb on - strand at 95.026 kb on - strand, within GFF4787 at 95.069 kb on + strand, within GFF4787 at 95.069 kb on + strand, within GFF4787 at 95.070 kb on - strand, within GFF4787 at 95.070 kb on - strand, within GFF4787 at 95.071 kb on + strand, within GFF4787 at 95.093 kb on - strand, within GFF4787 at 95.095 kb on - strand, within GFF4787 at 95.095 kb on - strand, within GFF4787 at 95.095 kb on - strand, within GFF4787 at 95.275 kb on - strand at 95.605 kb on - strand, within GFF4788 at 95.637 kb on - strand, within GFF4788 at 95.637 kb on - strand, within GFF4788 at 95.709 kb on - strand, within GFF4788 at 95.740 kb on - strand, within GFF4788 at 95.740 kb on - strand, within GFF4788 at 95.740 kb on - strand, within GFF4788 at 95.837 kb on - strand, within GFF4788
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 92,787 - -1.2 92,827 + -0.2 92,850 - +0.3 92,942 - +0.4 93,143 - GFF4785 0.30 +1.2 93,143 - GFF4785 0.30 +0.8 93,210 + GFF4785 0.41 -1.8 93,210 + GFF4785 0.41 -0.6 93,211 - GFF4785 0.41 +0.3 93,211 - GFF4785 0.41 +0.2 93,211 - GFF4785 0.41 +0.5 93,225 + GFF4785 0.44 -0.9 93,225 + GFF4785 0.44 -1.4 93,226 - GFF4785 0.44 -0.1 93,444 + GFF4785 0.81 -1.4 93,495 + GFF4785 0.89 -1.2 93,495 + GFF4785 0.89 -1.3 93,729 - GFF4786 0.12 +0.2 93,729 - GFF4786 0.12 +0.5 93,753 - GFF4786 0.14 +0.1 93,899 + GFF4786 0.24 -1.1 93,975 + GFF4786 0.29 -1.2 93,976 - GFF4786 0.29 +0.2 94,051 - GFF4786 0.35 +0.3 94,076 + GFF4786 0.36 -1.3 94,076 + GFF4786 0.36 -1.2 94,191 - GFF4786 0.44 -0.9 94,271 + GFF4786 0.50 -0.9 94,350 - GFF4786 0.55 +0.6 94,584 + GFF4786 0.72 -0.5 94,584 + GFF4786 0.72 -0.2 94,585 - GFF4786 0.72 -3.6 94,585 - GFF4786 0.72 +0.0 94,795 + GFF4786 0.87 -0.9 94,795 + GFF4786 0.87 -0.9 94,905 + -1.8 94,906 - -1.4 94,980 - -0.4 95,026 - GFF4787 0.13 +0.2 95,069 + GFF4787 0.31 -1.7 95,069 + GFF4787 0.31 -0.8 95,070 - GFF4787 0.31 +0.4 95,070 - GFF4787 0.31 +0.4 95,071 + GFF4787 0.32 -0.5 95,093 - GFF4787 0.41 +0.3 95,095 - GFF4787 0.42 -0.7 95,095 - GFF4787 0.42 +1.3 95,095 - GFF4787 0.42 +0.3 95,275 - -3.5 95,605 - GFF4788 0.33 -0.8 95,637 - GFF4788 0.36 -2.2 95,637 - GFF4788 0.36 -2.6 95,709 - GFF4788 0.44 -4.7 95,740 - GFF4788 0.47 -3.3 95,740 - GFF4788 0.47 -4.3 95,740 - GFF4788 0.47 -4.2 95,837 - GFF4788 0.56 -2.2
Or see this region's nucleotide sequence