Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4776 and GFF4777 are separated by 41 nucleotides GFF4777 and GFF4778 overlap by 4 nucleotides GFF4778 and GFF4779 are separated by 39 nucleotides
GFF4776 - LysR-family transcriptional regulator STM2912, at 84,379 to 85,290
GFF4776
GFF4777 - Gluconate permease, at 85,332 to 86,828
GFF4777
GFF4778 - Nucleoside-diphosphate-sugar epimerases, at 86,825 to 87,775
GFF4778
GFF4779 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 87,815 to 88,591
GFF4779
Position (kb)
85
86
87 Strain fitness (log2 ratio)
-2
-1
0
1 at 84.341 kb on - strand at 84.342 kb on + strand at 84.343 kb on - strand at 84.343 kb on - strand at 84.343 kb on - strand at 84.397 kb on - strand at 84.420 kb on - strand at 84.521 kb on + strand, within GFF4776 at 84.527 kb on + strand, within GFF4776 at 84.664 kb on - strand, within GFF4776 at 84.959 kb on + strand, within GFF4776 at 84.990 kb on + strand, within GFF4776 at 85.020 kb on - strand, within GFF4776 at 85.214 kb on + strand at 85.214 kb on + strand at 85.215 kb on - strand at 85.326 kb on - strand at 85.333 kb on + strand at 85.334 kb on - strand at 85.334 kb on - strand at 85.391 kb on + strand at 85.640 kb on + strand, within GFF4777 at 85.640 kb on + strand, within GFF4777 at 85.641 kb on - strand, within GFF4777 at 85.910 kb on + strand, within GFF4777 at 85.911 kb on - strand, within GFF4777 at 85.937 kb on + strand, within GFF4777 at 85.940 kb on - strand, within GFF4777 at 86.041 kb on - strand, within GFF4777 at 86.150 kb on + strand, within GFF4777 at 86.155 kb on + strand, within GFF4777 at 86.156 kb on - strand, within GFF4777 at 86.240 kb on - strand, within GFF4777 at 86.682 kb on - strand at 86.773 kb on - strand at 87.294 kb on + strand, within GFF4778 at 87.713 kb on + strand at 87.790 kb on + strand at 87.791 kb on - strand at 87.791 kb on - strand at 87.800 kb on + strand at 87.805 kb on + strand at 87.805 kb on + strand at 87.805 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 84,341 - -0.9 84,342 + -0.0 84,343 - +0.2 84,343 - -0.3 84,343 - +0.6 84,397 - -0.3 84,420 - +0.8 84,521 + GFF4776 0.16 +0.7 84,527 + GFF4776 0.16 +0.1 84,664 - GFF4776 0.31 -0.0 84,959 + GFF4776 0.64 -0.2 84,990 + GFF4776 0.67 -0.8 85,020 - GFF4776 0.70 +0.7 85,214 + +0.2 85,214 + +0.1 85,215 - -0.8 85,326 - +0.8 85,333 + +0.4 85,334 - -0.8 85,334 - -0.9 85,391 + +0.3 85,640 + GFF4777 0.21 +0.4 85,640 + GFF4777 0.21 -2.6 85,641 - GFF4777 0.21 -0.0 85,910 + GFF4777 0.39 +0.1 85,911 - GFF4777 0.39 +0.4 85,937 + GFF4777 0.40 +1.1 85,940 - GFF4777 0.41 +0.2 86,041 - GFF4777 0.47 +0.5 86,150 + GFF4777 0.55 -0.8 86,155 + GFF4777 0.55 +0.2 86,156 - GFF4777 0.55 +0.8 86,240 - GFF4777 0.61 -0.0 86,682 - +0.2 86,773 - +0.6 87,294 + GFF4778 0.49 +0.2 87,713 + -0.5 87,790 + -0.5 87,791 - -0.1 87,791 - -0.2 87,800 + -0.1 87,805 + +0.3 87,805 + +1.1 87,805 + +0.1
Or see this region's nucleotide sequence