Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4724 and GFF4725 are separated by 233 nucleotides GFF4725 and GFF4726 are separated by 61 nucleotides GFF4726 and GFF4727 are separated by 181 nucleotides
GFF4724 - [NiFe] hydrogenase metallocenter assembly protein HypE, at 32,256 to 33,266
GFF4724
GFF4725 - Formate hydrogenlyase transcriptional activator, at 33,500 to 35,563
GFF4725
GFF4726 - FIG00638716: hypothetical protein, at 35,625 to 35,969
GFF4726
GFF4727 - Manganese ABC transporter, periplasmic-binding protein SitA, at 36,151 to 37,068
GFF4727
Position (kb) 33
34
35
36 Strain fitness (log2 ratio) -2
-1
0
1 at 32.597 kb on + strand, within GFF4724 at 32.683 kb on + strand, within GFF4724 at 32.688 kb on - strand, within GFF4724 at 32.777 kb on + strand, within GFF4724 at 32.864 kb on - strand, within GFF4724 at 32.892 kb on - strand, within GFF4724 at 33.207 kb on + strand at 33.250 kb on + strand at 33.257 kb on + strand at 33.259 kb on + strand at 33.265 kb on - strand at 33.283 kb on + strand at 33.283 kb on + strand at 33.297 kb on + strand at 33.412 kb on + strand at 33.424 kb on + strand at 33.425 kb on - strand at 33.493 kb on + strand at 33.493 kb on + strand at 33.542 kb on - strand at 33.542 kb on - strand at 33.546 kb on + strand at 33.597 kb on + strand at 33.598 kb on - strand at 33.666 kb on - strand at 33.666 kb on - strand at 33.701 kb on + strand at 33.843 kb on - strand, within GFF4725 at 33.946 kb on + strand, within GFF4725 at 33.946 kb on + strand, within GFF4725 at 33.947 kb on - strand, within GFF4725 at 34.013 kb on + strand, within GFF4725 at 34.105 kb on - strand, within GFF4725 at 34.460 kb on - strand, within GFF4725 at 34.532 kb on + strand, within GFF4725 at 34.598 kb on - strand, within GFF4725 at 34.649 kb on + strand, within GFF4725 at 34.649 kb on + strand, within GFF4725 at 34.650 kb on - strand, within GFF4725 at 34.650 kb on - strand, within GFF4725 at 34.650 kb on - strand, within GFF4725 at 34.694 kb on - strand, within GFF4725 at 34.785 kb on + strand, within GFF4725 at 34.951 kb on - strand, within GFF4725 at 34.957 kb on + strand, within GFF4725 at 34.957 kb on + strand, within GFF4725 at 35.159 kb on - strand, within GFF4725 at 35.213 kb on - strand, within GFF4725 at 35.252 kb on - strand, within GFF4725 at 35.334 kb on + strand, within GFF4725 at 35.334 kb on + strand, within GFF4725 at 35.335 kb on - strand, within GFF4725 at 35.432 kb on - strand at 35.432 kb on - strand at 35.433 kb on + strand at 35.799 kb on - strand, within GFF4726 at 35.832 kb on - strand, within GFF4726 at 35.852 kb on - strand, within GFF4726 at 35.870 kb on - strand, within GFF4726 at 36.037 kb on + strand at 36.038 kb on - strand at 36.162 kb on - strand at 36.259 kb on - strand, within GFF4727 at 36.347 kb on - strand, within GFF4727 at 36.370 kb on - strand, within GFF4727 at 36.393 kb on + strand, within GFF4727 at 36.439 kb on + strand, within GFF4727 at 36.507 kb on - strand, within GFF4727 at 36.514 kb on + strand, within GFF4727
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 32,597 + GFF4724 0.34 +0.0 32,683 + GFF4724 0.42 -0.1 32,688 - GFF4724 0.43 +1.2 32,777 + GFF4724 0.52 -1.1 32,864 - GFF4724 0.60 +0.3 32,892 - GFF4724 0.63 -1.1 33,207 + -1.5 33,250 + +0.6 33,257 + +0.9 33,259 + +0.5 33,265 - -0.5 33,283 + +0.9 33,283 + -0.1 33,297 + +0.8 33,412 + -0.0 33,424 + -1.6 33,425 - -1.2 33,493 + +0.4 33,493 + +0.4 33,542 - +0.5 33,542 - -0.3 33,546 + +0.2 33,597 + -0.1 33,598 - -0.0 33,666 - +1.1 33,666 - +0.8 33,701 + -0.4 33,843 - GFF4725 0.17 -0.1 33,946 + GFF4725 0.22 -0.3 33,946 + GFF4725 0.22 +0.5 33,947 - GFF4725 0.22 +0.7 34,013 + GFF4725 0.25 -0.1 34,105 - GFF4725 0.29 +0.5 34,460 - GFF4725 0.47 -0.1 34,532 + GFF4725 0.50 -0.1 34,598 - GFF4725 0.53 -0.2 34,649 + GFF4725 0.56 +0.5 34,649 + GFF4725 0.56 -0.1 34,650 - GFF4725 0.56 -1.3 34,650 - GFF4725 0.56 +0.1 34,650 - GFF4725 0.56 +0.1 34,694 - GFF4725 0.58 +0.3 34,785 + GFF4725 0.62 -0.1 34,951 - GFF4725 0.70 +0.9 34,957 + GFF4725 0.71 -0.4 34,957 + GFF4725 0.71 -2.3 35,159 - GFF4725 0.80 +0.5 35,213 - GFF4725 0.83 +0.1 35,252 - GFF4725 0.85 +0.1 35,334 + GFF4725 0.89 -1.8 35,334 + GFF4725 0.89 -0.6 35,335 - GFF4725 0.89 +0.0 35,432 - -1.8 35,432 - +0.5 35,433 + +0.4 35,799 - GFF4726 0.50 -0.4 35,832 - GFF4726 0.60 -0.3 35,852 - GFF4726 0.66 -0.3 35,870 - GFF4726 0.71 +0.4 36,037 + +0.3 36,038 - +0.2 36,162 - +0.4 36,259 - GFF4727 0.12 -0.4 36,347 - GFF4727 0.21 -1.1 36,370 - GFF4727 0.24 +0.0 36,393 + GFF4727 0.26 -0.9 36,439 + GFF4727 0.31 +0.4 36,507 - GFF4727 0.39 +1.3 36,514 + GFF4727 0.40 +0.6
Or see this region's nucleotide sequence