Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4716 and GFF4717 overlap by 1 nucleotides GFF4717 and GFF4718 are separated by 145 nucleotides GFF4718 and GFF4719 are separated by 45 nucleotides GFF4719 and GFF4720 overlap by 40 nucleotides
GFF4716 - Formate hydrogenlyase subunit 3, at 26,327 to 28,153
GFF4716
GFF4717 - Formate hydrogenlyase subunit 2, at 28,153 to 28,761
GFF4717
GFF4718 - Formate hydrogenlyase regulatory protein HycA, at 28,907 to 29,368
GFF4718
GFF4719 - FIG01045287: hypothetical protein, at 29,414 to 29,617
GFF4719
GFF4720 - [NiFe] hydrogenase nickel incorporation protein HypA, at 29,578 to 29,934
GFF4720
Position (kb) 28
29 Strain fitness (log2 ratio) -2
-1
0
1 at 27.520 kb on + strand, within GFF4716 at 27.843 kb on - strand, within GFF4716 at 27.908 kb on - strand, within GFF4716 at 28.133 kb on - strand at 28.133 kb on - strand at 28.210 kb on - strand at 28.227 kb on + strand, within GFF4717 at 28.228 kb on - strand, within GFF4717 at 28.494 kb on + strand, within GFF4717 at 28.531 kb on - strand, within GFF4717 at 28.557 kb on + strand, within GFF4717 at 28.828 kb on + strand at 28.908 kb on + strand at 28.977 kb on + strand, within GFF4718 at 29.013 kb on + strand, within GFF4718 at 29.013 kb on + strand, within GFF4718 at 29.014 kb on - strand, within GFF4718 at 29.175 kb on + strand, within GFF4718 at 29.289 kb on + strand, within GFF4718 at 29.293 kb on + strand, within GFF4718 at 29.293 kb on + strand, within GFF4718 at 29.293 kb on + strand, within GFF4718 at 29.293 kb on + strand, within GFF4718 at 29.334 kb on + strand at 29.335 kb on - strand at 29.459 kb on + strand, within GFF4719 at 29.578 kb on - strand at 29.730 kb on - strand, within GFF4720
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 27,520 + GFF4716 0.65 -0.9 27,843 - GFF4716 0.83 -0.2 27,908 - GFF4716 0.87 -0.1 28,133 - -0.8 28,133 - +0.3 28,210 - +1.2 28,227 + GFF4717 0.12 +0.1 28,228 - GFF4717 0.12 -0.3 28,494 + GFF4717 0.56 +0.7 28,531 - GFF4717 0.62 -0.1 28,557 + GFF4717 0.66 +0.4 28,828 + -0.3 28,908 + +0.5 28,977 + GFF4718 0.15 +0.6 29,013 + GFF4718 0.23 -0.9 29,013 + GFF4718 0.23 +0.2 29,014 - GFF4718 0.23 -0.4 29,175 + GFF4718 0.58 +0.3 29,289 + GFF4718 0.83 +0.5 29,293 + GFF4718 0.84 +0.8 29,293 + GFF4718 0.84 +0.3 29,293 + GFF4718 0.84 -2.0 29,293 + GFF4718 0.84 +0.6 29,334 + +0.5 29,335 - +0.6 29,459 + GFF4719 0.22 +0.7 29,578 - +0.3 29,730 - GFF4720 0.43 +0.2
Or see this region's nucleotide sequence