Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4545 and GFF4546 are separated by 139 nucleotides GFF4546 and GFF4547 are separated by 10 nucleotides
GFF4545 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 328,615 to 329,826
GFF4545
GFF4546 - Rare lipoprotein A precursor, at 329,966 to 331,111
GFF4546
GFF4547 - Rod shape-determining protein RodA, at 331,122 to 332,234
GFF4547
Position (kb)
329
330
331
332 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 329.005 kb on - strand, within GFF4545 at 329.007 kb on - strand, within GFF4545 at 329.291 kb on - strand, within GFF4545 at 329.333 kb on + strand, within GFF4545 at 329.333 kb on + strand, within GFF4545 at 329.333 kb on + strand, within GFF4545 at 329.334 kb on - strand, within GFF4545 at 329.334 kb on - strand, within GFF4545 at 329.334 kb on - strand, within GFF4545 at 329.334 kb on - strand, within GFF4545 at 329.334 kb on - strand, within GFF4545 at 329.491 kb on - strand, within GFF4545 at 329.585 kb on + strand, within GFF4545 at 329.800 kb on - strand at 329.837 kb on - strand at 329.870 kb on + strand at 329.875 kb on + strand at 329.886 kb on + strand at 329.994 kb on - strand at 330.043 kb on + strand at 330.090 kb on - strand, within GFF4546 at 330.122 kb on + strand, within GFF4546 at 330.439 kb on + strand, within GFF4546 at 330.507 kb on - strand, within GFF4546 at 330.615 kb on - strand, within GFF4546 at 330.619 kb on + strand, within GFF4546 at 330.625 kb on + strand, within GFF4546 at 330.753 kb on + strand, within GFF4546 at 330.850 kb on - strand, within GFF4546 at 331.001 kb on + strand at 331.002 kb on - strand at 331.048 kb on + strand at 331.051 kb on + strand at 331.052 kb on - strand at 331.052 kb on - strand at 331.064 kb on + strand at 331.064 kb on + strand at 331.068 kb on - strand at 331.068 kb on - strand at 331.070 kb on + strand at 331.071 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 329,005 - GFF4545 0.32 -0.3 329,007 - GFF4545 0.32 -0.8 329,291 - GFF4545 0.56 -0.5 329,333 + GFF4545 0.59 -1.5 329,333 + GFF4545 0.59 -0.6 329,333 + GFF4545 0.59 +0.9 329,334 - GFF4545 0.59 -0.7 329,334 - GFF4545 0.59 -0.9 329,334 - GFF4545 0.59 -1.1 329,334 - GFF4545 0.59 +0.3 329,334 - GFF4545 0.59 -0.9 329,491 - GFF4545 0.72 -0.3 329,585 + GFF4545 0.80 -2.3 329,800 - -0.8 329,837 - -0.3 329,870 + -0.3 329,875 + -0.6 329,886 + -0.9 329,994 - -0.1 330,043 + -3.0 330,090 - GFF4546 0.11 -0.4 330,122 + GFF4546 0.14 -0.0 330,439 + GFF4546 0.41 -1.4 330,507 - GFF4546 0.47 -0.4 330,615 - GFF4546 0.57 -0.7 330,619 + GFF4546 0.57 +0.6 330,625 + GFF4546 0.58 -0.4 330,753 + GFF4546 0.69 -0.5 330,850 - GFF4546 0.77 +0.5 331,001 + -0.1 331,002 - -1.3 331,048 + -1.0 331,051 + -1.3 331,052 - -0.2 331,052 - -3.0 331,064 + -1.4 331,064 + -1.7 331,068 - -0.8 331,068 - -0.2 331,070 + -1.9 331,071 - -0.7
Or see this region's nucleotide sequence