Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4518 and GFF4519 are separated by 67 nucleotides GFF4519 and GFF4520 are separated by 21 nucleotides GFF4520 and GFF4521 are separated by 84 nucleotides GFF4521 and GFF4522 are separated by 82 nucleotides GFF4522 and GFF4523 are separated by 234 nucleotides
GFF4518 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.5.3), at 306,751 to 307,518
GFF4518
GFF4519 - Universal stress protein G, at 307,586 to 307,879
GFF4519
GFF4520 - FIG01045303: hypothetical protein, at 307,901 to 308,152
GFF4520
GFF4521 - Uncharacterized zinc-type alcohol dehydrogenase-like protein ybdR, at 308,237 to 309,475
GFF4521
GFF4522 - Regulator of nucleoside diphosphate kinase, at 309,558 to 309,968
GFF4522
GFF4523 - Ribonuclease I precursor (EC 3.1.27.6), at 310,203 to 311,009
GFF4523
Position (kb) 308
309
310 Strain fitness (log2 ratio) -1
0
1 at 307.261 kb on - strand, within GFF4518 at 307.352 kb on - strand, within GFF4518 at 307.491 kb on + strand at 307.574 kb on + strand at 307.588 kb on - strand at 307.673 kb on - strand, within GFF4519 at 307.763 kb on - strand, within GFF4519 at 307.898 kb on + strand at 308.011 kb on - strand, within GFF4520 at 308.011 kb on - strand, within GFF4520 at 308.189 kb on + strand at 308.200 kb on - strand at 308.253 kb on - strand at 308.663 kb on + strand, within GFF4521 at 308.698 kb on + strand, within GFF4521 at 308.699 kb on - strand, within GFF4521 at 308.725 kb on + strand, within GFF4521 at 309.080 kb on - strand, within GFF4521 at 309.317 kb on + strand, within GFF4521 at 309.317 kb on + strand, within GFF4521 at 309.318 kb on - strand, within GFF4521 at 309.318 kb on - strand, within GFF4521 at 309.319 kb on + strand, within GFF4521 at 309.319 kb on + strand, within GFF4521 at 309.319 kb on + strand, within GFF4521 at 309.320 kb on - strand, within GFF4521 at 309.546 kb on - strand at 309.896 kb on - strand, within GFF4522 at 309.924 kb on - strand, within GFF4522 at 309.951 kb on - strand at 309.989 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 307,261 - GFF4518 0.66 +0.6 307,352 - GFF4518 0.78 -0.7 307,491 + -0.6 307,574 + +0.0 307,588 - -0.0 307,673 - GFF4519 0.30 +0.5 307,763 - GFF4519 0.60 -0.3 307,898 + +1.1 308,011 - GFF4520 0.44 +0.5 308,011 - GFF4520 0.44 -0.2 308,189 + -0.3 308,200 - +0.5 308,253 - -0.1 308,663 + GFF4521 0.34 +0.2 308,698 + GFF4521 0.37 -1.3 308,699 - GFF4521 0.37 +0.6 308,725 + GFF4521 0.39 -0.7 309,080 - GFF4521 0.68 +0.6 309,317 + GFF4521 0.87 -1.2 309,317 + GFF4521 0.87 -1.6 309,318 - GFF4521 0.87 -1.1 309,318 - GFF4521 0.87 +0.0 309,319 + GFF4521 0.87 +0.3 309,319 + GFF4521 0.87 -1.0 309,319 + GFF4521 0.87 -0.3 309,320 - GFF4521 0.87 -0.9 309,546 - -0.3 309,896 - GFF4522 0.82 -0.7 309,924 - GFF4522 0.89 -0.3 309,951 - +0.1 309,989 + +0.0
Or see this region's nucleotide sequence