Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4463 and GFF4464 are separated by 238 nucleotides GFF4464 and GFF4465 are separated by 108 nucleotides GFF4465 and GFF4466 are separated by 50 nucleotides GFF4466 and GFF4467 are separated by 215 nucleotides
GFF4463 - copE1, at 248,434 to 248,565
GFF4463
GFF4464 - sensor histidine kinase, at 248,804 to 249,133
GFF4464
GFF4465 - Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA, at 249,242 to 249,421
GFF4465
GFF4466 - Putative HTH-type transcriptional regulator ykgD, at 249,472 to 250,326
GFF4466
GFF4467 - Putative Dihydrolipoamide dehydrogenase (EC 1.8.1.4); Mercuric ion reductase (EC 1.16.1.1); PF00070 family, FAD-dependent NAD(P)-disulphide oxidoreductase, at 250,542 to 251,867
GFF4467
Position (kb) 249
250
251 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 248.661 kb on - strand at 248.661 kb on - strand at 248.667 kb on - strand at 248.842 kb on + strand, within GFF4464 at 248.915 kb on + strand, within GFF4464 at 249.028 kb on + strand, within GFF4464 at 249.028 kb on + strand, within GFF4464 at 249.085 kb on + strand, within GFF4464 at 249.112 kb on + strand at 249.289 kb on - strand, within GFF4465 at 249.377 kb on + strand, within GFF4465 at 249.566 kb on + strand, within GFF4466 at 249.670 kb on - strand, within GFF4466 at 249.868 kb on - strand, within GFF4466 at 249.895 kb on + strand, within GFF4466 at 250.069 kb on - strand, within GFF4466 at 250.069 kb on - strand, within GFF4466 at 250.144 kb on - strand, within GFF4466 at 250.296 kb on + strand at 250.315 kb on - strand at 250.498 kb on - strand at 250.565 kb on + strand at 250.653 kb on - strand at 250.653 kb on - strand at 250.653 kb on - strand at 250.957 kb on + strand, within GFF4467 at 251.046 kb on - strand, within GFF4467 at 251.097 kb on - strand, within GFF4467 at 251.097 kb on - strand, within GFF4467 at 251.237 kb on - strand, within GFF4467 at 251.317 kb on + strand, within GFF4467 at 251.318 kb on - strand, within GFF4467
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 248,661 - -0.3 248,661 - -0.1 248,667 - -0.2 248,842 + GFF4464 0.12 -3.1 248,915 + GFF4464 0.34 -1.3 249,028 + GFF4464 0.68 -0.8 249,028 + GFF4464 0.68 +0.2 249,085 + GFF4464 0.85 +0.0 249,112 + +0.3 249,289 - GFF4465 0.26 -0.7 249,377 + GFF4465 0.75 -0.1 249,566 + GFF4466 0.11 +0.7 249,670 - GFF4466 0.23 +0.5 249,868 - GFF4466 0.46 -0.6 249,895 + GFF4466 0.49 -0.1 250,069 - GFF4466 0.70 -1.3 250,069 - GFF4466 0.70 +0.1 250,144 - GFF4466 0.79 +0.1 250,296 + -1.0 250,315 - -0.1 250,498 - -0.0 250,565 + -1.6 250,653 - -0.0 250,653 - +0.2 250,653 - +0.1 250,957 + GFF4467 0.31 -2.9 251,046 - GFF4467 0.38 -0.2 251,097 - GFF4467 0.42 +0.8 251,097 - GFF4467 0.42 -1.2 251,237 - GFF4467 0.52 +0.5 251,317 + GFF4467 0.58 +0.8 251,318 - GFF4467 0.59 +0.3
Or see this region's nucleotide sequence