Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4409 and GFF4410 are separated by 85 nucleotides GFF4410 and GFF4411 are separated by 32 nucleotides GFF4411 and GFF4412 are separated by 154 nucleotides GFF4412 and GFF4413 overlap by 30 nucleotides
GFF4409 - YbbM seven transmembrane helix protein, at 193,364 to 194,143
GFF4409
GFF4410 - FIG000875: Thioredoxin domain-containing protein EC-YbbN, at 194,229 to 195,110
GFF4410
GFF4411 - Putative NAD(P)-dependent oxidoreductase EC-YbbO, at 195,143 to 195,913
GFF4411
GFF4412 - Arylesterase precursor (EC 3.1.1.2), at 196,068 to 196,682
GFF4412
GFF4413 - Uncharacterized metabolite ABC transporter in Enterobacteriaceae, ATP-binding protein EC-YbbA, at 196,653 to 197,339
GFF4413
Position (kb) 195
196 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 194.145 kb on - strand at 194.204 kb on - strand at 194.231 kb on - strand at 194.233 kb on + strand at 194.245 kb on + strand at 194.246 kb on - strand at 194.390 kb on + strand, within GFF4410 at 194.390 kb on + strand, within GFF4410 at 194.712 kb on + strand, within GFF4410 at 194.839 kb on + strand, within GFF4410 at 194.930 kb on - strand, within GFF4410 at 194.999 kb on - strand, within GFF4410 at 195.031 kb on - strand at 195.060 kb on - strand at 195.114 kb on + strand at 195.266 kb on + strand, within GFF4411 at 195.276 kb on - strand, within GFF4411 at 195.278 kb on + strand, within GFF4411 at 195.435 kb on - strand, within GFF4411 at 195.566 kb on + strand, within GFF4411 at 195.654 kb on + strand, within GFF4411 at 195.655 kb on - strand, within GFF4411 at 195.687 kb on + strand, within GFF4411 at 195.960 kb on + strand at 195.961 kb on - strand at 195.961 kb on - strand at 195.972 kb on - strand at 195.998 kb on - strand at 196.011 kb on - strand at 196.011 kb on - strand at 196.011 kb on - strand at 196.014 kb on + strand at 196.014 kb on + strand at 196.015 kb on - strand at 196.018 kb on + strand at 196.018 kb on + strand at 196.019 kb on - strand at 196.019 kb on - strand at 196.019 kb on - strand at 196.019 kb on - strand at 196.038 kb on + strand at 196.112 kb on + strand at 196.176 kb on + strand, within GFF4412 at 196.176 kb on + strand, within GFF4412 at 196.176 kb on + strand, within GFF4412 at 196.229 kb on + strand, within GFF4412 at 196.259 kb on - strand, within GFF4412 at 196.345 kb on - strand, within GFF4412 at 196.380 kb on + strand, within GFF4412 at 196.472 kb on + strand, within GFF4412 at 196.482 kb on + strand, within GFF4412 at 196.526 kb on + strand, within GFF4412 at 196.527 kb on - strand, within GFF4412 at 196.593 kb on + strand, within GFF4412
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 194,145 - +0.8 194,204 - +0.4 194,231 - -0.3 194,233 + +1.2 194,245 + +0.5 194,246 - +0.5 194,390 + GFF4410 0.18 +0.7 194,390 + GFF4410 0.18 +0.4 194,712 + GFF4410 0.55 -0.8 194,839 + GFF4410 0.69 -0.7 194,930 - GFF4410 0.79 -0.2 194,999 - GFF4410 0.87 +0.4 195,031 - -0.7 195,060 - +0.8 195,114 + +0.5 195,266 + GFF4411 0.16 -2.4 195,276 - GFF4411 0.17 -2.2 195,278 + GFF4411 0.18 -1.5 195,435 - GFF4411 0.38 -1.8 195,566 + GFF4411 0.55 -2.4 195,654 + GFF4411 0.66 -2.9 195,655 - GFF4411 0.66 -2.7 195,687 + GFF4411 0.71 -0.8 195,960 + -2.0 195,961 - -0.8 195,961 - -1.3 195,972 - -0.4 195,998 - +0.5 196,011 - -0.0 196,011 - -0.6 196,011 - +1.6 196,014 + -1.4 196,014 + -2.5 196,015 - +1.3 196,018 + -2.4 196,018 + -0.8 196,019 - +1.1 196,019 - +0.6 196,019 - -0.5 196,019 - +0.9 196,038 + -2.1 196,112 + -1.8 196,176 + GFF4412 0.18 -1.1 196,176 + GFF4412 0.18 -1.2 196,176 + GFF4412 0.18 -1.2 196,229 + GFF4412 0.26 -1.8 196,259 - GFF4412 0.31 -0.0 196,345 - GFF4412 0.45 +0.2 196,380 + GFF4412 0.51 -0.3 196,472 + GFF4412 0.66 -0.6 196,482 + GFF4412 0.67 -1.8 196,526 + GFF4412 0.74 -2.4 196,527 - GFF4412 0.75 -1.2 196,593 + GFF4412 0.85 +0.2
Or see this region's nucleotide sequence