Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4396 and GFF4397 are separated by 48 nucleotides GFF4397 and GFF4398 are separated by 77 nucleotides GFF4398 and GFF4399 are separated by 11 nucleotides
GFF4396 - Inosine-guanosine kinase (EC 2.7.1.73), at 179,211 to 180,515
GFF4396
GFF4397 - POTASSIUM/PROTON ANTIPORTER ROSB, at 180,564 to 182,240
GFF4397
GFF4398 - hypothetical protein, at 182,318 to 182,443
GFF4398
GFF4399 - Fosmidomycin resistance protein, at 182,455 to 183,675
GFF4399
Position (kb) 180
181
182
183 Strain fitness (log2 ratio) -2
-1
0
1 at 179.595 kb on + strand, within GFF4396 at 179.607 kb on - strand, within GFF4396 at 179.644 kb on + strand, within GFF4396 at 179.697 kb on - strand, within GFF4396 at 179.720 kb on + strand, within GFF4396 at 179.794 kb on - strand, within GFF4396 at 179.808 kb on - strand, within GFF4396 at 180.055 kb on + strand, within GFF4396 at 180.074 kb on - strand, within GFF4396 at 180.081 kb on + strand, within GFF4396 at 180.081 kb on + strand, within GFF4396 at 180.082 kb on - strand, within GFF4396 at 180.082 kb on - strand, within GFF4396 at 180.082 kb on - strand, within GFF4396 at 180.083 kb on + strand, within GFF4396 at 180.083 kb on + strand, within GFF4396 at 180.084 kb on - strand, within GFF4396 at 180.084 kb on - strand, within GFF4396 at 180.146 kb on + strand, within GFF4396 at 180.201 kb on - strand, within GFF4396 at 180.201 kb on - strand, within GFF4396 at 180.237 kb on + strand, within GFF4396 at 180.327 kb on + strand, within GFF4396 at 180.336 kb on - strand, within GFF4396 at 180.357 kb on - strand, within GFF4396 at 180.404 kb on + strand at 180.405 kb on - strand at 180.408 kb on + strand at 180.409 kb on - strand at 180.674 kb on - strand at 180.692 kb on - strand at 180.990 kb on - strand, within GFF4397 at 181.109 kb on + strand, within GFF4397 at 181.110 kb on - strand, within GFF4397 at 181.307 kb on + strand, within GFF4397 at 181.354 kb on - strand, within GFF4397 at 181.421 kb on + strand, within GFF4397 at 181.422 kb on - strand, within GFF4397 at 181.458 kb on - strand, within GFF4397 at 181.458 kb on - strand, within GFF4397 at 181.659 kb on - strand, within GFF4397 at 181.659 kb on - strand, within GFF4397 at 181.899 kb on + strand, within GFF4397 at 181.899 kb on + strand, within GFF4397 at 182.463 kb on + strand at 182.660 kb on + strand, within GFF4399 at 182.661 kb on - strand, within GFF4399 at 182.778 kb on + strand, within GFF4399 at 182.918 kb on + strand, within GFF4399 at 182.951 kb on + strand, within GFF4399 at 182.980 kb on + strand, within GFF4399 at 183.006 kb on + strand, within GFF4399
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 179,595 + GFF4396 0.29 -0.6 179,607 - GFF4396 0.30 +0.0 179,644 + GFF4396 0.33 -0.0 179,697 - GFF4396 0.37 -0.5 179,720 + GFF4396 0.39 +0.7 179,794 - GFF4396 0.45 +1.0 179,808 - GFF4396 0.46 +0.4 180,055 + GFF4396 0.65 -1.5 180,074 - GFF4396 0.66 -0.3 180,081 + GFF4396 0.67 -0.8 180,081 + GFF4396 0.67 -0.8 180,082 - GFF4396 0.67 -0.0 180,082 - GFF4396 0.67 -0.7 180,082 - GFF4396 0.67 +1.0 180,083 + GFF4396 0.67 -0.8 180,083 + GFF4396 0.67 +0.3 180,084 - GFF4396 0.67 -0.4 180,084 - GFF4396 0.67 +0.4 180,146 + GFF4396 0.72 -0.6 180,201 - GFF4396 0.76 +0.3 180,201 - GFF4396 0.76 -0.8 180,237 + GFF4396 0.79 +0.4 180,327 + GFF4396 0.86 -0.3 180,336 - GFF4396 0.86 +0.3 180,357 - GFF4396 0.88 -0.4 180,404 + +0.4 180,405 - -0.1 180,408 + -1.4 180,409 - -0.2 180,674 - -0.0 180,692 - -0.4 180,990 - GFF4397 0.25 -0.1 181,109 + GFF4397 0.32 -1.1 181,110 - GFF4397 0.33 -1.1 181,307 + GFF4397 0.44 -0.9 181,354 - GFF4397 0.47 +0.0 181,421 + GFF4397 0.51 +0.3 181,422 - GFF4397 0.51 -1.3 181,458 - GFF4397 0.53 -0.8 181,458 - GFF4397 0.53 +0.4 181,659 - GFF4397 0.65 +0.0 181,659 - GFF4397 0.65 +0.0 181,899 + GFF4397 0.80 +0.7 181,899 + GFF4397 0.80 -0.1 182,463 + -0.2 182,660 + GFF4399 0.17 -0.1 182,661 - GFF4399 0.17 -0.3 182,778 + GFF4399 0.26 +0.3 182,918 + GFF4399 0.38 -2.4 182,951 + GFF4399 0.41 +0.1 182,980 + GFF4399 0.43 -0.7 183,006 + GFF4399 0.45 -0.4
Or see this region's nucleotide sequence