Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4297 and GFF4298 are separated by 212 nucleotides GFF4298 and GFF4299 are separated by 46 nucleotides GFF4299 and GFF4300 are separated by 14 nucleotides
GFF4297 - Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3), at 81,766 to 83,061
GFF4297
GFF4298 - hypothetical protein, at 83,274 to 83,423
GFF4298
GFF4299 - Branched-chain amino acid transport system carrier protein, at 83,470 to 84,789
GFF4299
GFF4300 - Proline-specific permease proY, at 84,804 to 86,234
GFF4300
Position (kb)
83
84
85 Strain fitness (log2 ratio)
-1
0
1 at 82.498 kb on - strand, within GFF4297 at 82.516 kb on + strand, within GFF4297 at 82.604 kb on + strand, within GFF4297 at 82.605 kb on - strand, within GFF4297 at 82.669 kb on - strand, within GFF4297 at 82.719 kb on - strand, within GFF4297 at 82.719 kb on - strand, within GFF4297 at 82.782 kb on + strand, within GFF4297 at 82.885 kb on + strand, within GFF4297 at 83.109 kb on - strand at 83.277 kb on + strand at 83.551 kb on - strand at 83.551 kb on - strand at 83.842 kb on + strand, within GFF4299 at 84.010 kb on + strand, within GFF4299 at 84.288 kb on + strand, within GFF4299 at 84.288 kb on + strand, within GFF4299 at 84.289 kb on - strand, within GFF4299 at 84.289 kb on - strand, within GFF4299 at 84.394 kb on + strand, within GFF4299 at 84.564 kb on + strand, within GFF4299 at 84.802 kb on - strand at 84.802 kb on - strand at 84.802 kb on - strand at 84.802 kb on - strand at 84.807 kb on + strand at 84.808 kb on - strand at 84.879 kb on - strand at 84.883 kb on + strand at 84.884 kb on - strand at 84.926 kb on - strand at 85.005 kb on + strand, within GFF4300 at 85.008 kb on - strand, within GFF4300 at 85.031 kb on + strand, within GFF4300 at 85.032 kb on - strand, within GFF4300 at 85.032 kb on - strand, within GFF4300 at 85.032 kb on - strand, within GFF4300 at 85.032 kb on - strand, within GFF4300 at 85.147 kb on - strand, within GFF4300 at 85.244 kb on - strand, within GFF4300 at 85.343 kb on + strand, within GFF4300 at 85.343 kb on + strand, within GFF4300 at 85.344 kb on - strand, within GFF4300 at 85.346 kb on + strand, within GFF4300 at 85.346 kb on + strand, within GFF4300 at 85.572 kb on - strand, within GFF4300 at 85.572 kb on - strand, within GFF4300 at 85.625 kb on + strand, within GFF4300 at 85.632 kb on - strand, within GFF4300 at 85.784 kb on + strand, within GFF4300
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 82,498 - GFF4297 0.56 -0.0 82,516 + GFF4297 0.58 +0.2 82,604 + GFF4297 0.65 +0.4 82,605 - GFF4297 0.65 +0.1 82,669 - GFF4297 0.70 -0.4 82,719 - GFF4297 0.74 +0.5 82,719 - GFF4297 0.74 -1.1 82,782 + GFF4297 0.78 +0.3 82,885 + GFF4297 0.86 +0.2 83,109 - +0.6 83,277 + +1.1 83,551 - +0.7 83,551 - +1.0 83,842 + GFF4299 0.28 +0.1 84,010 + GFF4299 0.41 +1.2 84,288 + GFF4299 0.62 +0.5 84,288 + GFF4299 0.62 +0.5 84,289 - GFF4299 0.62 +0.8 84,289 - GFF4299 0.62 +0.1 84,394 + GFF4299 0.70 +0.8 84,564 + GFF4299 0.83 +0.4 84,802 - +0.1 84,802 - +0.1 84,802 - +0.4 84,802 - +0.7 84,807 + -0.2 84,808 - +0.6 84,879 - -0.8 84,883 + -0.7 84,884 - -1.5 84,926 - -0.5 85,005 + GFF4300 0.14 +0.5 85,008 - GFF4300 0.14 -0.4 85,031 + GFF4300 0.16 -0.7 85,032 - GFF4300 0.16 -0.4 85,032 - GFF4300 0.16 -0.1 85,032 - GFF4300 0.16 +0.0 85,032 - GFF4300 0.16 -0.7 85,147 - GFF4300 0.24 -0.6 85,244 - GFF4300 0.31 -0.2 85,343 + GFF4300 0.38 -0.1 85,343 + GFF4300 0.38 -0.3 85,344 - GFF4300 0.38 -0.6 85,346 + GFF4300 0.38 +0.1 85,346 + GFF4300 0.38 -0.0 85,572 - GFF4300 0.54 -0.7 85,572 - GFF4300 0.54 +0.6 85,625 + GFF4300 0.57 -1.5 85,632 - GFF4300 0.58 -0.3 85,784 + GFF4300 0.68 -0.7
Or see this region's nucleotide sequence