Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4263 and GFF4264 are separated by 122 nucleotides GFF4264 and GFF4265 are separated by 39 nucleotides
GFF4263 - Methylisocitrate lyase (EC 4.1.3.30), at 48,189 to 49,076
GFF4263
GFF4264 - 2-methylcitrate synthase (EC 2.3.3.5), at 49,199 to 50,368
GFF4264
GFF4265 - 2-methylcitrate dehydratase (EC 4.2.1.79), at 50,408 to 51,859
GFF4265
Position (kb)
49
50
51 Strain fitness (log2 ratio)
-1
0
1
2 at 48.315 kb on + strand, within GFF4263 at 48.316 kb on - strand, within GFF4263 at 48.474 kb on - strand, within GFF4263 at 48.474 kb on - strand, within GFF4263 at 48.601 kb on + strand, within GFF4263 at 48.717 kb on + strand, within GFF4263 at 49.023 kb on + strand at 49.023 kb on + strand at 49.023 kb on + strand at 49.024 kb on - strand at 49.074 kb on + strand at 49.082 kb on - strand at 49.128 kb on - strand at 49.154 kb on + strand at 49.154 kb on + strand at 49.155 kb on - strand at 49.172 kb on - strand at 49.172 kb on - strand at 49.297 kb on + strand at 49.433 kb on + strand, within GFF4264 at 49.434 kb on - strand, within GFF4264 at 49.434 kb on - strand, within GFF4264 at 49.579 kb on + strand, within GFF4264 at 49.580 kb on - strand, within GFF4264 at 49.580 kb on - strand, within GFF4264 at 49.656 kb on - strand, within GFF4264 at 49.676 kb on - strand, within GFF4264 at 49.772 kb on - strand, within GFF4264 at 49.788 kb on - strand, within GFF4264 at 50.108 kb on + strand, within GFF4264 at 50.109 kb on - strand, within GFF4264 at 50.193 kb on - strand, within GFF4264 at 50.273 kb on - strand at 50.318 kb on + strand at 50.318 kb on + strand at 50.318 kb on + strand at 50.367 kb on - strand at 50.407 kb on + strand at 50.476 kb on + strand at 50.523 kb on - strand at 50.752 kb on + strand, within GFF4265 at 51.234 kb on - strand, within GFF4265
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 48,315 + GFF4263 0.14 -0.2 48,316 - GFF4263 0.14 -0.2 48,474 - GFF4263 0.32 -0.3 48,474 - GFF4263 0.32 +0.4 48,601 + GFF4263 0.46 -0.4 48,717 + GFF4263 0.59 -0.8 49,023 + -0.4 49,023 + -0.8 49,023 + -1.1 49,024 - -0.2 49,074 + +0.0 49,082 - +0.5 49,128 - -0.2 49,154 + -1.4 49,154 + +0.3 49,155 - -0.0 49,172 - -0.3 49,172 - +0.3 49,297 + +0.3 49,433 + GFF4264 0.20 +0.5 49,434 - GFF4264 0.20 +0.3 49,434 - GFF4264 0.20 -0.6 49,579 + GFF4264 0.32 +0.1 49,580 - GFF4264 0.33 +0.2 49,580 - GFF4264 0.33 -0.3 49,656 - GFF4264 0.39 +0.5 49,676 - GFF4264 0.41 +0.2 49,772 - GFF4264 0.49 +0.7 49,788 - GFF4264 0.50 -0.0 50,108 + GFF4264 0.78 -0.3 50,109 - GFF4264 0.78 -0.3 50,193 - GFF4264 0.85 +2.1 50,273 - -0.0 50,318 + -0.1 50,318 + +0.2 50,318 + -0.6 50,367 - +0.2 50,407 + +0.4 50,476 + +0.3 50,523 - +1.0 50,752 + GFF4265 0.24 -1.0 51,234 - GFF4265 0.57 -0.4
Or see this region's nucleotide sequence