Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF420 and GFF421 overlap by 4 nucleotides GFF421 and GFF422 are separated by 284 nucleotides
GFF420 - L-seryl-tRNA(Sec) selenium transferase (EC 2.9.1.1), at 138,994 to 140,385
GFF420
GFF421 - Selenocysteine-specific translation elongation factor, at 140,382 to 142,232
GFF421
GFF422 - Putative sugar kinase, at 142,517 to 143,398
GFF422
Position (kb)
140
141
142
143 Strain fitness (log2 ratio)
-1
0
1
2 at 139.446 kb on + strand, within GFF420 at 139.592 kb on + strand, within GFF420 at 139.672 kb on - strand, within GFF420 at 139.678 kb on - strand, within GFF420 at 139.749 kb on + strand, within GFF420 at 139.793 kb on + strand, within GFF420 at 139.794 kb on - strand, within GFF420 at 139.801 kb on + strand, within GFF420 at 139.876 kb on - strand, within GFF420 at 140.006 kb on + strand, within GFF420 at 140.007 kb on - strand, within GFF420 at 140.014 kb on + strand, within GFF420 at 140.195 kb on + strand, within GFF420 at 140.387 kb on + strand at 140.408 kb on - strand at 140.547 kb on - strand at 140.580 kb on - strand, within GFF421 at 140.580 kb on - strand, within GFF421 at 140.768 kb on - strand, within GFF421 at 140.788 kb on - strand, within GFF421 at 140.809 kb on + strand, within GFF421 at 140.840 kb on + strand, within GFF421 at 141.002 kb on - strand, within GFF421 at 141.017 kb on + strand, within GFF421 at 141.020 kb on + strand, within GFF421 at 141.075 kb on + strand, within GFF421 at 141.101 kb on + strand, within GFF421 at 141.239 kb on + strand, within GFF421 at 141.239 kb on + strand, within GFF421 at 141.239 kb on + strand, within GFF421 at 141.240 kb on - strand, within GFF421 at 141.552 kb on - strand, within GFF421 at 141.659 kb on + strand, within GFF421 at 141.672 kb on + strand, within GFF421 at 141.801 kb on - strand, within GFF421 at 141.833 kb on + strand, within GFF421 at 142.014 kb on + strand, within GFF421 at 142.214 kb on - strand at 142.342 kb on + strand at 142.395 kb on - strand at 142.443 kb on + strand at 142.728 kb on + strand, within GFF422 at 142.869 kb on - strand, within GFF422 at 142.891 kb on + strand, within GFF422 at 142.892 kb on - strand, within GFF422 at 142.898 kb on - strand, within GFF422 at 142.982 kb on - strand, within GFF422
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 139,446 + GFF420 0.32 +0.8 139,592 + GFF420 0.43 +0.5 139,672 - GFF420 0.49 -0.4 139,678 - GFF420 0.49 +1.3 139,749 + GFF420 0.54 +0.7 139,793 + GFF420 0.57 +1.0 139,794 - GFF420 0.57 +1.1 139,801 + GFF420 0.58 +1.1 139,876 - GFF420 0.63 +0.8 140,006 + GFF420 0.73 +1.4 140,007 - GFF420 0.73 +1.6 140,014 + GFF420 0.73 +0.7 140,195 + GFF420 0.86 +1.1 140,387 + +0.3 140,408 - +1.0 140,547 - +1.9 140,580 - GFF421 0.11 +1.3 140,580 - GFF421 0.11 +0.1 140,768 - GFF421 0.21 +0.7 140,788 - GFF421 0.22 -0.6 140,809 + GFF421 0.23 +1.9 140,840 + GFF421 0.25 +0.6 141,002 - GFF421 0.33 +0.9 141,017 + GFF421 0.34 +0.5 141,020 + GFF421 0.34 +1.2 141,075 + GFF421 0.37 +1.8 141,101 + GFF421 0.39 +0.8 141,239 + GFF421 0.46 +1.0 141,239 + GFF421 0.46 +0.8 141,239 + GFF421 0.46 +0.9 141,240 - GFF421 0.46 +0.6 141,552 - GFF421 0.63 +0.8 141,659 + GFF421 0.69 -1.4 141,672 + GFF421 0.70 +1.3 141,801 - GFF421 0.77 -0.9 141,833 + GFF421 0.78 +1.3 142,014 + GFF421 0.88 -0.1 142,214 - +1.2 142,342 + -1.6 142,395 - -0.1 142,443 + +1.5 142,728 + GFF422 0.24 +0.6 142,869 - GFF422 0.40 +0.0 142,891 + GFF422 0.42 -1.2 142,892 - GFF422 0.43 +0.1 142,898 - GFF422 0.43 -1.0 142,982 - GFF422 0.53 +0.8
Or see this region's nucleotide sequence