Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF419 and GFF420 are separated by 98 nucleotides GFF420 and GFF421 overlap by 4 nucleotides
GFF419 - Uncharacterized GST-like protein yibF, at 138,287 to 138,895
GFF419
GFF420 - L-seryl-tRNA(Sec) selenium transferase (EC 2.9.1.1), at 138,994 to 140,385
GFF420
GFF421 - Selenocysteine-specific translation elongation factor, at 140,382 to 142,232
GFF421
Position (kb) 138
139
140
141 Strain fitness (log2 ratio) -1
0
1
2 at 137.994 kb on - strand at 137.994 kb on - strand at 138.006 kb on - strand at 138.251 kb on - strand at 138.251 kb on - strand at 138.263 kb on - strand at 138.301 kb on + strand at 138.356 kb on - strand, within GFF419 at 138.358 kb on + strand, within GFF419 at 138.358 kb on + strand, within GFF419 at 138.359 kb on - strand, within GFF419 at 138.386 kb on + strand, within GFF419 at 138.413 kb on + strand, within GFF419 at 138.462 kb on - strand, within GFF419 at 138.493 kb on + strand, within GFF419 at 138.493 kb on + strand, within GFF419 at 138.586 kb on + strand, within GFF419 at 138.587 kb on - strand, within GFF419 at 138.691 kb on + strand, within GFF419 at 138.692 kb on - strand, within GFF419 at 138.903 kb on + strand at 138.904 kb on - strand at 138.904 kb on - strand at 138.928 kb on + strand at 138.928 kb on + strand at 138.929 kb on - strand at 138.940 kb on + strand at 139.012 kb on - strand at 139.036 kb on - strand at 139.446 kb on + strand, within GFF420 at 139.592 kb on + strand, within GFF420 at 139.672 kb on - strand, within GFF420 at 139.678 kb on - strand, within GFF420 at 139.749 kb on + strand, within GFF420 at 139.793 kb on + strand, within GFF420 at 139.794 kb on - strand, within GFF420 at 139.801 kb on + strand, within GFF420 at 139.876 kb on - strand, within GFF420 at 140.006 kb on + strand, within GFF420 at 140.007 kb on - strand, within GFF420 at 140.014 kb on + strand, within GFF420 at 140.195 kb on + strand, within GFF420 at 140.387 kb on + strand at 140.408 kb on - strand at 140.547 kb on - strand at 140.580 kb on - strand, within GFF421 at 140.580 kb on - strand, within GFF421 at 140.768 kb on - strand, within GFF421 at 140.788 kb on - strand, within GFF421 at 140.809 kb on + strand, within GFF421 at 140.840 kb on + strand, within GFF421 at 141.002 kb on - strand, within GFF421 at 141.017 kb on + strand, within GFF421 at 141.020 kb on + strand, within GFF421 at 141.075 kb on + strand, within GFF421 at 141.101 kb on + strand, within GFF421 at 141.239 kb on + strand, within GFF421 at 141.239 kb on + strand, within GFF421 at 141.239 kb on + strand, within GFF421 at 141.240 kb on - strand, within GFF421
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 137,994 - +0.2 137,994 - -0.0 138,006 - -0.2 138,251 - +0.4 138,251 - +0.3 138,263 - -0.0 138,301 + -0.4 138,356 - GFF419 0.11 -1.2 138,358 + GFF419 0.12 -0.2 138,358 + GFF419 0.12 -0.8 138,359 - GFF419 0.12 +0.0 138,386 + GFF419 0.16 +0.5 138,413 + GFF419 0.21 +0.9 138,462 - GFF419 0.29 +0.9 138,493 + GFF419 0.34 -0.6 138,493 + GFF419 0.34 -0.9 138,586 + GFF419 0.49 -1.0 138,587 - GFF419 0.49 +0.0 138,691 + GFF419 0.66 -0.8 138,692 - GFF419 0.67 +1.5 138,903 + -1.2 138,904 - -0.2 138,904 - -0.7 138,928 + -1.4 138,928 + +0.1 138,929 - +0.9 138,940 + +1.2 139,012 - +0.6 139,036 - +0.0 139,446 + GFF420 0.32 +0.8 139,592 + GFF420 0.43 +0.5 139,672 - GFF420 0.49 -0.4 139,678 - GFF420 0.49 +1.3 139,749 + GFF420 0.54 +0.7 139,793 + GFF420 0.57 +1.0 139,794 - GFF420 0.57 +1.1 139,801 + GFF420 0.58 +1.1 139,876 - GFF420 0.63 +0.8 140,006 + GFF420 0.73 +1.4 140,007 - GFF420 0.73 +1.6 140,014 + GFF420 0.73 +0.7 140,195 + GFF420 0.86 +1.1 140,387 + +0.3 140,408 - +1.0 140,547 - +1.9 140,580 - GFF421 0.11 +1.3 140,580 - GFF421 0.11 +0.1 140,768 - GFF421 0.21 +0.7 140,788 - GFF421 0.22 -0.6 140,809 + GFF421 0.23 +1.9 140,840 + GFF421 0.25 +0.6 141,002 - GFF421 0.33 +0.9 141,017 + GFF421 0.34 +0.5 141,020 + GFF421 0.34 +1.2 141,075 + GFF421 0.37 +1.8 141,101 + GFF421 0.39 +0.8 141,239 + GFF421 0.46 +1.0 141,239 + GFF421 0.46 +0.8 141,239 + GFF421 0.46 +0.9 141,240 - GFF421 0.46 +0.6
Or see this region's nucleotide sequence