Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4050 and GFF4051 are separated by 15 nucleotides GFF4051 and GFF4052 overlap by 4 nucleotides GFF4052 and GFF4053 overlap by 14 nucleotides
GFF4050 - Protein ImpG/VasA, at 12,133 to 14,016
GFF4050
GFF4051 - Uncharacterized protein ImpF, at 14,032 to 14,526
GFF4051
GFF4052 - Protein of avirulence locus ImpE, at 14,523 to 15,347
GFF4052
GFF4053 - FIG074102: hypothetical protein, at 15,334 to 16,224
GFF4053
Position (kb) 14
15
16 Strain fitness (log2 ratio) -1
0
1
2 at 13.769 kb on + strand, within GFF4050 at 13.883 kb on + strand at 13.973 kb on + strand at 13.974 kb on - strand at 13.992 kb on - strand at 14.030 kb on + strand at 14.134 kb on + strand, within GFF4051 at 14.142 kb on + strand, within GFF4051 at 14.144 kb on + strand, within GFF4051 at 14.144 kb on + strand, within GFF4051 at 14.144 kb on + strand, within GFF4051 at 14.144 kb on + strand, within GFF4051 at 14.145 kb on - strand, within GFF4051 at 14.145 kb on - strand, within GFF4051 at 14.267 kb on + strand, within GFF4051 at 14.268 kb on - strand, within GFF4051 at 14.268 kb on - strand, within GFF4051 at 14.272 kb on - strand, within GFF4051 at 14.272 kb on - strand, within GFF4051 at 14.272 kb on - strand, within GFF4051 at 14.508 kb on + strand at 14.818 kb on - strand, within GFF4052 at 14.843 kb on - strand, within GFF4052 at 15.137 kb on - strand, within GFF4052 at 15.159 kb on + strand, within GFF4052 at 15.159 kb on + strand, within GFF4052 at 15.159 kb on + strand, within GFF4052 at 15.291 kb on - strand at 15.405 kb on + strand at 15.406 kb on - strand at 15.408 kb on + strand at 15.448 kb on - strand, within GFF4053 at 15.627 kb on - strand, within GFF4053 at 15.627 kb on - strand, within GFF4053 at 15.726 kb on - strand, within GFF4053 at 15.841 kb on - strand, within GFF4053 at 15.970 kb on - strand, within GFF4053 at 15.990 kb on + strand, within GFF4053 at 16.151 kb on - strand at 16.157 kb on + strand at 16.158 kb on - strand at 16.158 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 13,769 + GFF4050 0.87 +1.4 13,883 + +0.3 13,973 + -0.6 13,974 - -0.4 13,992 - -1.0 14,030 + -0.2 14,134 + GFF4051 0.21 +1.0 14,142 + GFF4051 0.22 +0.5 14,144 + GFF4051 0.23 -0.3 14,144 + GFF4051 0.23 -0.0 14,144 + GFF4051 0.23 +0.2 14,144 + GFF4051 0.23 -0.1 14,145 - GFF4051 0.23 -0.8 14,145 - GFF4051 0.23 +0.1 14,267 + GFF4051 0.47 +0.9 14,268 - GFF4051 0.48 +0.2 14,268 - GFF4051 0.48 +0.3 14,272 - GFF4051 0.48 +0.0 14,272 - GFF4051 0.48 -0.0 14,272 - GFF4051 0.48 +0.1 14,508 + -1.3 14,818 - GFF4052 0.36 +2.3 14,843 - GFF4052 0.39 +0.7 15,137 - GFF4052 0.74 +0.2 15,159 + GFF4052 0.77 -0.8 15,159 + GFF4052 0.77 +0.4 15,159 + GFF4052 0.77 +1.1 15,291 - -0.6 15,405 + +0.6 15,406 - -0.4 15,408 + +0.3 15,448 - GFF4053 0.13 -0.4 15,627 - GFF4053 0.33 +0.7 15,627 - GFF4053 0.33 -0.1 15,726 - GFF4053 0.44 -0.3 15,841 - GFF4053 0.57 +0.2 15,970 - GFF4053 0.71 -1.3 15,990 + GFF4053 0.74 -0.1 16,151 - +0.2 16,157 + +1.0 16,158 - -0.1 16,158 - -0.3
Or see this region's nucleotide sequence