Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4044 and GFF4045 are separated by 88 nucleotides GFF4045 and GFF4046 overlap by 4 nucleotides GFF4046 and GFF4047 are separated by 54 nucleotides
GFF4044 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 6,772 to 7,527
GFF4044
GFF4045 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 7,616 to 8,284
GFF4045
GFF4046 - Ribonuclease HI (EC 3.1.26.4), at 8,281 to 8,748
GFF4046
GFF4047 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 8,803 to 9,543
GFF4047
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6.681 kb on - strand at 6.721 kb on - strand at 6.721 kb on - strand at 6.763 kb on + strand at 6.952 kb on - strand, within GFF4044 at 7.017 kb on + strand, within GFF4044 at 7.017 kb on + strand, within GFF4044 at 7.018 kb on - strand, within GFF4044 at 7.022 kb on - strand, within GFF4044 at 7.245 kb on + strand, within GFF4044 at 7.246 kb on - strand, within GFF4044 at 7.401 kb on + strand, within GFF4044 at 7.402 kb on - strand, within GFF4044 at 7.402 kb on - strand, within GFF4044 at 7.513 kb on - strand at 7.527 kb on + strand at 7.528 kb on - strand at 7.530 kb on - strand at 7.532 kb on + strand at 7.631 kb on + strand at 7.806 kb on + strand, within GFF4045 at 7.806 kb on + strand, within GFF4045 at 7.855 kb on + strand, within GFF4045 at 7.856 kb on - strand, within GFF4045 at 8.073 kb on + strand, within GFF4045 at 8.074 kb on - strand, within GFF4045 at 8.074 kb on - strand, within GFF4045 at 8.079 kb on - strand, within GFF4045 at 8.079 kb on - strand, within GFF4045 at 8.097 kb on + strand, within GFF4045 at 8.098 kb on - strand, within GFF4045 at 8.164 kb on + strand, within GFF4045 at 8.165 kb on - strand, within GFF4045 at 8.216 kb on - strand, within GFF4045 at 8.283 kb on - strand at 8.304 kb on + strand at 8.800 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 6,681 - +0.1 6,721 - -1.1 6,721 - -0.7 6,763 + -1.3 6,952 - GFF4044 0.24 -0.3 7,017 + GFF4044 0.32 +0.2 7,017 + GFF4044 0.32 -1.1 7,018 - GFF4044 0.33 -0.4 7,022 - GFF4044 0.33 -1.3 7,245 + GFF4044 0.63 +0.0 7,246 - GFF4044 0.63 +0.1 7,401 + GFF4044 0.83 -0.7 7,402 - GFF4044 0.83 -0.3 7,402 - GFF4044 0.83 -0.1 7,513 - +0.1 7,527 + -0.1 7,528 - +0.3 7,530 - -2.8 7,532 + -0.5 7,631 + -0.2 7,806 + GFF4045 0.28 +1.3 7,806 + GFF4045 0.28 +0.5 7,855 + GFF4045 0.36 +0.7 7,856 - GFF4045 0.36 +0.3 8,073 + GFF4045 0.68 -0.5 8,074 - GFF4045 0.68 +0.1 8,074 - GFF4045 0.68 -0.9 8,079 - GFF4045 0.69 +0.2 8,079 - GFF4045 0.69 +1.0 8,097 + GFF4045 0.72 +0.5 8,098 - GFF4045 0.72 +0.7 8,164 + GFF4045 0.82 +0.1 8,165 - GFF4045 0.82 -0.4 8,216 - GFF4045 0.90 -0.3 8,283 - -0.1 8,304 + +0.1 8,800 + +0.2
Or see this region's nucleotide sequence