Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4043 and GFF4044 are separated by 218 nucleotides GFF4044 and GFF4045 are separated by 88 nucleotides GFF4045 and GFF4046 overlap by 4 nucleotides
GFF4043 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 5,333 to 6,553
GFF4043
GFF4044 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 6,772 to 7,527
GFF4044
GFF4045 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 7,616 to 8,284
GFF4045
GFF4046 - Ribonuclease HI (EC 3.1.26.4), at 8,281 to 8,748
GFF4046
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5.777 kb on - strand, within GFF4043 at 5.777 kb on - strand, within GFF4043 at 5.782 kb on - strand, within GFF4043 at 5.782 kb on - strand, within GFF4043 at 5.849 kb on - strand, within GFF4043 at 5.945 kb on + strand, within GFF4043 at 5.995 kb on + strand, within GFF4043 at 5.996 kb on - strand, within GFF4043 at 6.107 kb on - strand, within GFF4043 at 6.171 kb on - strand, within GFF4043 at 6.218 kb on - strand, within GFF4043 at 6.231 kb on - strand, within GFF4043 at 6.333 kb on - strand, within GFF4043 at 6.356 kb on - strand, within GFF4043 at 6.384 kb on + strand, within GFF4043 at 6.385 kb on - strand, within GFF4043 at 6.385 kb on - strand, within GFF4043 at 6.388 kb on + strand, within GFF4043 at 6.389 kb on - strand, within GFF4043 at 6.389 kb on - strand, within GFF4043 at 6.389 kb on - strand, within GFF4043 at 6.391 kb on - strand, within GFF4043 at 6.411 kb on - strand, within GFF4043 at 6.424 kb on - strand, within GFF4043 at 6.424 kb on - strand, within GFF4043 at 6.431 kb on - strand, within GFF4043 at 6.431 kb on - strand, within GFF4043 at 6.442 kb on - strand at 6.536 kb on - strand at 6.681 kb on - strand at 6.721 kb on - strand at 6.721 kb on - strand at 6.763 kb on + strand at 6.952 kb on - strand, within GFF4044 at 7.017 kb on + strand, within GFF4044 at 7.017 kb on + strand, within GFF4044 at 7.018 kb on - strand, within GFF4044 at 7.022 kb on - strand, within GFF4044 at 7.245 kb on + strand, within GFF4044 at 7.246 kb on - strand, within GFF4044 at 7.401 kb on + strand, within GFF4044 at 7.402 kb on - strand, within GFF4044 at 7.402 kb on - strand, within GFF4044 at 7.513 kb on - strand at 7.527 kb on + strand at 7.528 kb on - strand at 7.530 kb on - strand at 7.532 kb on + strand at 7.631 kb on + strand at 7.806 kb on + strand, within GFF4045 at 7.806 kb on + strand, within GFF4045 at 7.855 kb on + strand, within GFF4045 at 7.856 kb on - strand, within GFF4045 at 8.073 kb on + strand, within GFF4045 at 8.074 kb on - strand, within GFF4045 at 8.074 kb on - strand, within GFF4045 at 8.079 kb on - strand, within GFF4045 at 8.079 kb on - strand, within GFF4045 at 8.097 kb on + strand, within GFF4045 at 8.098 kb on - strand, within GFF4045 at 8.164 kb on + strand, within GFF4045 at 8.165 kb on - strand, within GFF4045 at 8.216 kb on - strand, within GFF4045 at 8.283 kb on - strand at 8.304 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 5,777 - GFF4043 0.36 -1.7 5,777 - GFF4043 0.36 +0.4 5,782 - GFF4043 0.37 -0.4 5,782 - GFF4043 0.37 -0.5 5,849 - GFF4043 0.42 -1.5 5,945 + GFF4043 0.50 -0.8 5,995 + GFF4043 0.54 +0.2 5,996 - GFF4043 0.54 -1.0 6,107 - GFF4043 0.63 +0.4 6,171 - GFF4043 0.69 -1.6 6,218 - GFF4043 0.72 +1.4 6,231 - GFF4043 0.74 -1.8 6,333 - GFF4043 0.82 +0.1 6,356 - GFF4043 0.84 -0.2 6,384 + GFF4043 0.86 -1.6 6,385 - GFF4043 0.86 +0.2 6,385 - GFF4043 0.86 -1.3 6,388 + GFF4043 0.86 +1.1 6,389 - GFF4043 0.86 +0.5 6,389 - GFF4043 0.86 +0.2 6,389 - GFF4043 0.86 +0.1 6,391 - GFF4043 0.87 -0.2 6,411 - GFF4043 0.88 +0.3 6,424 - GFF4043 0.89 +0.2 6,424 - GFF4043 0.89 +0.2 6,431 - GFF4043 0.90 +0.6 6,431 - GFF4043 0.90 +0.4 6,442 - -1.7 6,536 - +1.0 6,681 - +0.1 6,721 - -1.1 6,721 - -0.7 6,763 + -1.3 6,952 - GFF4044 0.24 -0.3 7,017 + GFF4044 0.32 +0.2 7,017 + GFF4044 0.32 -1.1 7,018 - GFF4044 0.33 -0.4 7,022 - GFF4044 0.33 -1.3 7,245 + GFF4044 0.63 +0.0 7,246 - GFF4044 0.63 +0.1 7,401 + GFF4044 0.83 -0.7 7,402 - GFF4044 0.83 -0.3 7,402 - GFF4044 0.83 -0.1 7,513 - +0.1 7,527 + -0.1 7,528 - +0.3 7,530 - -2.8 7,532 + -0.5 7,631 + -0.2 7,806 + GFF4045 0.28 +1.3 7,806 + GFF4045 0.28 +0.5 7,855 + GFF4045 0.36 +0.7 7,856 - GFF4045 0.36 +0.3 8,073 + GFF4045 0.68 -0.5 8,074 - GFF4045 0.68 +0.1 8,074 - GFF4045 0.68 -0.9 8,079 - GFF4045 0.69 +0.2 8,079 - GFF4045 0.69 +1.0 8,097 + GFF4045 0.72 +0.5 8,098 - GFF4045 0.72 +0.7 8,164 + GFF4045 0.82 +0.1 8,165 - GFF4045 0.82 -0.4 8,216 - GFF4045 0.90 -0.3 8,283 - -0.1 8,304 + +0.1
Or see this region's nucleotide sequence