Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4044

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4043 and GFF4044 are separated by 218 nucleotidesGFF4044 and GFF4045 are separated by 88 nucleotidesGFF4045 and GFF4046 overlap by 4 nucleotides GFF4043 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 5,333 to 6,553 GFF4043 GFF4044 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 6,772 to 7,527 GFF4044 GFF4045 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 7,616 to 8,284 GFF4045 GFF4046 - Ribonuclease HI (EC 3.1.26.4), at 8,281 to 8,748 GFF4046 Position (kb) 6 7 8Strain fitness (log2 ratio) -3 -2 -1 0 1at 5.777 kb on - strand, within GFF4043at 5.777 kb on - strand, within GFF4043at 5.782 kb on - strand, within GFF4043at 5.782 kb on - strand, within GFF4043at 5.849 kb on - strand, within GFF4043at 5.945 kb on + strand, within GFF4043at 5.995 kb on + strand, within GFF4043at 5.996 kb on - strand, within GFF4043at 6.107 kb on - strand, within GFF4043at 6.171 kb on - strand, within GFF4043at 6.218 kb on - strand, within GFF4043at 6.231 kb on - strand, within GFF4043at 6.333 kb on - strand, within GFF4043at 6.356 kb on - strand, within GFF4043at 6.384 kb on + strand, within GFF4043at 6.385 kb on - strand, within GFF4043at 6.385 kb on - strand, within GFF4043at 6.388 kb on + strand, within GFF4043at 6.389 kb on - strand, within GFF4043at 6.389 kb on - strand, within GFF4043at 6.389 kb on - strand, within GFF4043at 6.391 kb on - strand, within GFF4043at 6.411 kb on - strand, within GFF4043at 6.424 kb on - strand, within GFF4043at 6.424 kb on - strand, within GFF4043at 6.431 kb on - strand, within GFF4043at 6.431 kb on - strand, within GFF4043at 6.442 kb on - strandat 6.536 kb on - strandat 6.681 kb on - strandat 6.721 kb on - strandat 6.721 kb on - strandat 6.763 kb on + strandat 6.952 kb on - strand, within GFF4044at 7.017 kb on + strand, within GFF4044at 7.017 kb on + strand, within GFF4044at 7.018 kb on - strand, within GFF4044at 7.022 kb on - strand, within GFF4044at 7.245 kb on + strand, within GFF4044at 7.246 kb on - strand, within GFF4044at 7.401 kb on + strand, within GFF4044at 7.402 kb on - strand, within GFF4044at 7.402 kb on - strand, within GFF4044at 7.513 kb on - strandat 7.527 kb on + strandat 7.528 kb on - strandat 7.530 kb on - strandat 7.532 kb on + strandat 7.631 kb on + strandat 7.806 kb on + strand, within GFF4045at 7.806 kb on + strand, within GFF4045at 7.855 kb on + strand, within GFF4045at 7.856 kb on - strand, within GFF4045at 8.073 kb on + strand, within GFF4045at 8.074 kb on - strand, within GFF4045at 8.074 kb on - strand, within GFF4045at 8.079 kb on - strand, within GFF4045at 8.079 kb on - strand, within GFF4045at 8.097 kb on + strand, within GFF4045at 8.098 kb on - strand, within GFF4045at 8.164 kb on + strand, within GFF4045at 8.165 kb on - strand, within GFF4045at 8.216 kb on - strand, within GFF4045at 8.283 kb on - strandat 8.304 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
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5,777 - GFF4043 0.36 -1.7
5,777 - GFF4043 0.36 +0.4
5,782 - GFF4043 0.37 -0.4
5,782 - GFF4043 0.37 -0.5
5,849 - GFF4043 0.42 -1.5
5,945 + GFF4043 0.50 -0.8
5,995 + GFF4043 0.54 +0.2
5,996 - GFF4043 0.54 -1.0
6,107 - GFF4043 0.63 +0.4
6,171 - GFF4043 0.69 -1.6
6,218 - GFF4043 0.72 +1.4
6,231 - GFF4043 0.74 -1.8
6,333 - GFF4043 0.82 +0.1
6,356 - GFF4043 0.84 -0.2
6,384 + GFF4043 0.86 -1.6
6,385 - GFF4043 0.86 +0.2
6,385 - GFF4043 0.86 -1.3
6,388 + GFF4043 0.86 +1.1
6,389 - GFF4043 0.86 +0.5
6,389 - GFF4043 0.86 +0.2
6,389 - GFF4043 0.86 +0.1
6,391 - GFF4043 0.87 -0.2
6,411 - GFF4043 0.88 +0.3
6,424 - GFF4043 0.89 +0.2
6,424 - GFF4043 0.89 +0.2
6,431 - GFF4043 0.90 +0.6
6,431 - GFF4043 0.90 +0.4
6,442 - -1.7
6,536 - +1.0
6,681 - +0.1
6,721 - -1.1
6,721 - -0.7
6,763 + -1.3
6,952 - GFF4044 0.24 -0.3
7,017 + GFF4044 0.32 +0.2
7,017 + GFF4044 0.32 -1.1
7,018 - GFF4044 0.33 -0.4
7,022 - GFF4044 0.33 -1.3
7,245 + GFF4044 0.63 +0.0
7,246 - GFF4044 0.63 +0.1
7,401 + GFF4044 0.83 -0.7
7,402 - GFF4044 0.83 -0.3
7,402 - GFF4044 0.83 -0.1
7,513 - +0.1
7,527 + -0.1
7,528 - +0.3
7,530 - -2.8
7,532 + -0.5
7,631 + -0.2
7,806 + GFF4045 0.28 +1.3
7,806 + GFF4045 0.28 +0.5
7,855 + GFF4045 0.36 +0.7
7,856 - GFF4045 0.36 +0.3
8,073 + GFF4045 0.68 -0.5
8,074 - GFF4045 0.68 +0.1
8,074 - GFF4045 0.68 -0.9
8,079 - GFF4045 0.69 +0.2
8,079 - GFF4045 0.69 +1.0
8,097 + GFF4045 0.72 +0.5
8,098 - GFF4045 0.72 +0.7
8,164 + GFF4045 0.82 +0.1
8,165 - GFF4045 0.82 -0.4
8,216 - GFF4045 0.90 -0.3
8,283 - -0.1
8,304 + +0.1

Or see this region's nucleotide sequence