Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3883

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3882 and GFF3883 are separated by 21 nucleotidesGFF3883 and GFF3884 overlap by 4 nucleotides GFF3882 - Lipopolysaccharide biosynthesis protein WzxC, at 159,381 to 160,892 GFF3882 GFF3883 - Colanic acid biosysnthesis protein WcaK, at 160,914 to 162,194 GFF3883 GFF3884 - Colanic acid biosynthesis glycosyl transferase WcaL, at 162,191 to 163,411 GFF3884 Position (kb) 160 161 162 163Strain fitness (log2 ratio) -1 0 1 2 3at 160.074 kb on - strand, within GFF3882at 160.084 kb on - strand, within GFF3882at 160.150 kb on - strand, within GFF3882at 160.152 kb on - strand, within GFF3882at 160.162 kb on - strand, within GFF3882at 160.194 kb on - strand, within GFF3882at 160.273 kb on - strand, within GFF3882at 160.273 kb on - strand, within GFF3882at 160.275 kb on - strand, within GFF3882at 160.485 kb on - strand, within GFF3882at 160.485 kb on - strand, within GFF3882at 160.654 kb on - strand, within GFF3882at 160.656 kb on - strand, within GFF3882at 160.741 kb on + strandat 160.773 kb on - strandat 160.902 kb on - strandat 161.266 kb on - strand, within GFF3883at 161.368 kb on - strand, within GFF3883at 161.577 kb on + strand, within GFF3883at 161.765 kb on + strand, within GFF3883at 161.818 kb on - strand, within GFF3883at 161.973 kb on + strand, within GFF3883at 161.982 kb on - strand, within GFF3883at 162.007 kb on + strand, within GFF3883at 162.007 kb on + strand, within GFF3883at 162.266 kb on - strandat 162.322 kb on + strand, within GFF3884at 162.322 kb on + strand, within GFF3884at 162.345 kb on - strand, within GFF3884at 162.422 kb on - strand, within GFF3884at 162.557 kb on - strand, within GFF3884at 162.557 kb on - strand, within GFF3884at 162.653 kb on - strand, within GFF3884at 162.658 kb on + strand, within GFF3884at 163.139 kb on - strand, within GFF3884at 163.185 kb on + strand, within GFF3884at 163.189 kb on + strand, within GFF3884

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
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160,074 - GFF3882 0.46 -0.1
160,084 - GFF3882 0.46 -0.4
160,150 - GFF3882 0.51 -0.4
160,152 - GFF3882 0.51 -0.2
160,162 - GFF3882 0.52 +0.2
160,194 - GFF3882 0.54 +0.0
160,273 - GFF3882 0.59 +1.0
160,273 - GFF3882 0.59 +0.0
160,275 - GFF3882 0.59 -1.4
160,485 - GFF3882 0.73 -0.3
160,485 - GFF3882 0.73 -0.1
160,654 - GFF3882 0.84 -0.9
160,656 - GFF3882 0.84 +0.0
160,741 + +3.5
160,773 - -0.6
160,902 - +0.7
161,266 - GFF3883 0.27 +0.4
161,368 - GFF3883 0.35 +0.1
161,577 + GFF3883 0.52 +0.7
161,765 + GFF3883 0.66 +0.8
161,818 - GFF3883 0.71 +0.2
161,973 + GFF3883 0.83 +2.8
161,982 - GFF3883 0.83 -0.2
162,007 + GFF3883 0.85 +2.8
162,007 + GFF3883 0.85 +2.7
162,266 - -0.6
162,322 + GFF3884 0.11 +1.8
162,322 + GFF3884 0.11 +1.7
162,345 - GFF3884 0.13 +0.8
162,422 - GFF3884 0.19 -1.0
162,557 - GFF3884 0.30 +0.3
162,557 - GFF3884 0.30 +1.0
162,653 - GFF3884 0.38 -0.5
162,658 + GFF3884 0.38 +2.8
163,139 - GFF3884 0.78 -0.8
163,185 + GFF3884 0.81 +3.0
163,189 + GFF3884 0.82 +3.0

Or see this region's nucleotide sequence