Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3758 and GFF3759 are separated by 9 nucleotides GFF3759 and GFF3760 are separated by 106 nucleotides
GFF3758 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 25,723 to 26,817
GFF3758
GFF3759 - ABC transporter, periplasmic substrate-binding protein, at 26,827 to 28,632
GFF3759
GFF3760 - Rtn protein, at 28,739 to 30,295
GFF3760
Position (kb) 26
27
28
29 Strain fitness (log2 ratio) -2
-1
0
1 at 26.147 kb on + strand, within GFF3758 at 26.165 kb on + strand, within GFF3758 at 26.223 kb on - strand, within GFF3758 at 26.225 kb on + strand, within GFF3758 at 26.293 kb on - strand, within GFF3758 at 26.406 kb on + strand, within GFF3758 at 26.490 kb on - strand, within GFF3758 at 26.490 kb on - strand, within GFF3758 at 26.808 kb on - strand at 26.826 kb on - strand at 26.826 kb on - strand at 26.828 kb on + strand at 26.871 kb on - strand at 26.895 kb on + strand at 26.896 kb on - strand at 26.896 kb on - strand at 26.896 kb on - strand at 26.896 kb on - strand at 26.900 kb on - strand at 26.959 kb on - strand at 26.959 kb on - strand at 26.959 kb on - strand at 26.960 kb on + strand at 26.960 kb on + strand at 26.960 kb on + strand at 26.961 kb on - strand at 26.979 kb on - strand at 26.981 kb on + strand at 27.106 kb on - strand, within GFF3759 at 27.235 kb on - strand, within GFF3759 at 27.259 kb on - strand, within GFF3759 at 27.259 kb on - strand, within GFF3759 at 27.360 kb on + strand, within GFF3759 at 27.471 kb on - strand, within GFF3759 at 27.578 kb on + strand, within GFF3759 at 27.605 kb on + strand, within GFF3759 at 27.618 kb on + strand, within GFF3759 at 27.820 kb on + strand, within GFF3759 at 27.882 kb on - strand, within GFF3759 at 27.965 kb on + strand, within GFF3759 at 28.090 kb on - strand, within GFF3759 at 28.090 kb on - strand, within GFF3759 at 28.167 kb on + strand, within GFF3759 at 28.169 kb on + strand, within GFF3759 at 28.169 kb on + strand, within GFF3759 at 28.369 kb on - strand, within GFF3759 at 28.399 kb on + strand, within GFF3759 at 28.424 kb on + strand, within GFF3759 at 28.446 kb on + strand, within GFF3759 at 28.446 kb on + strand, within GFF3759 at 28.447 kb on - strand, within GFF3759 at 28.530 kb on + strand at 28.824 kb on + strand at 28.825 kb on - strand at 28.992 kb on + strand, within GFF3760 at 28.997 kb on + strand, within GFF3760 at 29.107 kb on - strand, within GFF3760 at 29.188 kb on + strand, within GFF3760 at 29.322 kb on - strand, within GFF3760 at 29.490 kb on - strand, within GFF3760 at 29.523 kb on + strand, within GFF3760 at 29.523 kb on + strand, within GFF3760 at 29.524 kb on - strand, within GFF3760 at 29.528 kb on - strand, within GFF3760 at 29.617 kb on - strand, within GFF3760
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 26,147 + GFF3758 0.39 +0.5 26,165 + GFF3758 0.40 -1.6 26,223 - GFF3758 0.46 -0.2 26,225 + GFF3758 0.46 -0.0 26,293 - GFF3758 0.52 +0.6 26,406 + GFF3758 0.62 +0.3 26,490 - GFF3758 0.70 -0.2 26,490 - GFF3758 0.70 +0.4 26,808 - -0.1 26,826 - +0.1 26,826 - -1.3 26,828 + +0.5 26,871 - -0.4 26,895 + -1.1 26,896 - -1.2 26,896 - -0.0 26,896 - -0.1 26,896 - -0.1 26,900 - -0.5 26,959 - -1.4 26,959 - -1.8 26,959 - +0.2 26,960 + +0.4 26,960 + +0.1 26,960 + -0.6 26,961 - -2.0 26,979 - +0.3 26,981 + -0.5 27,106 - GFF3759 0.15 +0.3 27,235 - GFF3759 0.23 -0.4 27,259 - GFF3759 0.24 +0.3 27,259 - GFF3759 0.24 +0.2 27,360 + GFF3759 0.30 +0.1 27,471 - GFF3759 0.36 -0.3 27,578 + GFF3759 0.42 -0.7 27,605 + GFF3759 0.43 -0.2 27,618 + GFF3759 0.44 +0.3 27,820 + GFF3759 0.55 -1.1 27,882 - GFF3759 0.58 -1.1 27,965 + GFF3759 0.63 +0.2 28,090 - GFF3759 0.70 -1.5 28,090 - GFF3759 0.70 -0.1 28,167 + GFF3759 0.74 -0.4 28,169 + GFF3759 0.74 -0.3 28,169 + GFF3759 0.74 -0.8 28,369 - GFF3759 0.85 +0.2 28,399 + GFF3759 0.87 -2.1 28,424 + GFF3759 0.88 -0.9 28,446 + GFF3759 0.90 -0.1 28,446 + GFF3759 0.90 -0.4 28,447 - GFF3759 0.90 -0.2 28,530 + +0.1 28,824 + -0.4 28,825 - +0.4 28,992 + GFF3760 0.16 -1.1 28,997 + GFF3760 0.17 -0.1 29,107 - GFF3760 0.24 +0.3 29,188 + GFF3760 0.29 -0.8 29,322 - GFF3760 0.37 -1.8 29,490 - GFF3760 0.48 +0.2 29,523 + GFF3760 0.50 +0.9 29,523 + GFF3760 0.50 -0.9 29,524 - GFF3760 0.50 +0.9 29,528 - GFF3760 0.51 -1.2 29,617 - GFF3760 0.56 +0.7
Or see this region's nucleotide sequence