Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3674 and GFF3675 overlap by 4 nucleotides GFF3675 and GFF3676 are separated by 210 nucleotides GFF3676 and GFF3677 overlap by 21 nucleotides GFF3677 and GFF3678 are separated by 135 nucleotides
GFF3674 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 26,085 to 26,267
GFF3674
GFF3675 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 26,264 to 27,010
GFF3675
GFF3676 - FIG00510082: hypothetical protein, at 27,221 to 28,114
GFF3676
GFF3677 - Membrane Protein Functionally coupled to the MukBEF Chromosome Partitioning Mechanism, at 28,094 to 28,870
GFF3677
GFF3678 - S-adenosylmethionine-dependent methyltransferase Functionally Coupled to the MukBEF Chromosome Partitioning Mechanism, at 29,006 to 29,809
GFF3678
Position (kb) 27
28
29 Strain fitness (log2 ratio) -2
-1
0
1 at 27.057 kb on - strand at 27.187 kb on - strand at 27.275 kb on - strand at 27.318 kb on + strand, within GFF3676 at 27.318 kb on + strand, within GFF3676 at 27.319 kb on - strand, within GFF3676 at 27.320 kb on + strand, within GFF3676 at 27.320 kb on + strand, within GFF3676 at 27.321 kb on - strand, within GFF3676 at 27.559 kb on - strand, within GFF3676 at 27.585 kb on + strand, within GFF3676 at 27.635 kb on - strand, within GFF3676 at 28.116 kb on - strand at 28.573 kb on + strand, within GFF3677 at 28.574 kb on - strand, within GFF3677 at 28.627 kb on + strand, within GFF3677 at 28.629 kb on + strand, within GFF3677 at 28.716 kb on - strand, within GFF3677 at 28.832 kb on + strand at 28.833 kb on - strand at 28.837 kb on + strand at 28.895 kb on + strand at 28.895 kb on + strand at 28.896 kb on - strand at 28.896 kb on - strand at 28.897 kb on + strand at 28.898 kb on - strand at 28.917 kb on + strand at 28.920 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 27,057 - -1.2 27,187 - +0.4 27,275 - -0.3 27,318 + GFF3676 0.11 -0.2 27,318 + GFF3676 0.11 +0.2 27,319 - GFF3676 0.11 -0.8 27,320 + GFF3676 0.11 +0.9 27,320 + GFF3676 0.11 -0.3 27,321 - GFF3676 0.11 +1.0 27,559 - GFF3676 0.38 +0.8 27,585 + GFF3676 0.41 +1.6 27,635 - GFF3676 0.46 -0.4 28,116 - -0.3 28,573 + GFF3677 0.62 +0.3 28,574 - GFF3677 0.62 -2.2 28,627 + GFF3677 0.69 -0.5 28,629 + GFF3677 0.69 -0.3 28,716 - GFF3677 0.80 -0.5 28,832 + -0.4 28,833 - -0.6 28,837 + -1.2 28,895 + +0.3 28,895 + -1.4 28,896 - -0.4 28,896 - +0.1 28,897 + -2.5 28,898 - -0.5 28,917 + -0.1 28,920 + -0.0
Or see this region's nucleotide sequence