Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF35 and GFF36 are separated by 75 nucleotides GFF36 and GFF37 are separated by 180 nucleotides
GFF35 - Lysine decarboxylase 2, constitutive (EC 4.1.1.18), at 10,926 to 13,067
GFF35
GFF36 - Chitinase (EC 3.2.1.14), at 13,143 to 14,906
GFF36
GFF37 - Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2), at 15,087 to 16,046
GFF37
Position (kb) 13
14
15 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 12.145 kb on + strand, within GFF35 at 12.201 kb on + strand, within GFF35 at 12.342 kb on - strand, within GFF35 at 12.342 kb on - strand, within GFF35 at 12.355 kb on - strand, within GFF35 at 12.359 kb on + strand, within GFF35 at 12.379 kb on + strand, within GFF35 at 12.379 kb on + strand, within GFF35 at 12.379 kb on + strand, within GFF35 at 12.383 kb on + strand, within GFF35 at 12.396 kb on - strand, within GFF35 at 12.425 kb on + strand, within GFF35 at 12.425 kb on + strand, within GFF35 at 12.426 kb on - strand, within GFF35 at 12.426 kb on - strand, within GFF35 at 12.426 kb on - strand, within GFF35 at 12.427 kb on + strand, within GFF35 at 12.427 kb on + strand, within GFF35 at 12.428 kb on - strand, within GFF35 at 12.428 kb on - strand, within GFF35 at 12.634 kb on + strand, within GFF35 at 12.923 kb on + strand at 13.025 kb on + strand at 13.026 kb on - strand at 13.099 kb on - strand at 13.136 kb on + strand at 13.144 kb on + strand at 13.192 kb on + strand at 13.211 kb on + strand at 13.246 kb on + strand at 13.247 kb on - strand at 13.326 kb on - strand, within GFF36 at 13.424 kb on - strand, within GFF36 at 13.514 kb on + strand, within GFF36 at 13.517 kb on - strand, within GFF36 at 13.517 kb on - strand, within GFF36 at 13.517 kb on - strand, within GFF36 at 13.708 kb on + strand, within GFF36 at 13.709 kb on - strand, within GFF36 at 13.709 kb on - strand, within GFF36 at 13.709 kb on - strand, within GFF36 at 13.743 kb on - strand, within GFF36 at 13.754 kb on - strand, within GFF36 at 13.774 kb on - strand, within GFF36 at 13.927 kb on + strand, within GFF36 at 14.040 kb on + strand, within GFF36 at 14.097 kb on + strand, within GFF36 at 14.098 kb on - strand, within GFF36 at 14.102 kb on + strand, within GFF36 at 14.236 kb on + strand, within GFF36 at 14.242 kb on + strand, within GFF36 at 14.243 kb on - strand, within GFF36 at 14.246 kb on + strand, within GFF36 at 14.334 kb on + strand, within GFF36 at 14.335 kb on - strand, within GFF36 at 14.481 kb on - strand, within GFF36 at 14.481 kb on - strand, within GFF36 at 14.483 kb on + strand, within GFF36 at 14.495 kb on + strand, within GFF36 at 14.586 kb on - strand, within GFF36 at 14.814 kb on - strand at 14.925 kb on + strand at 14.964 kb on + strand at 14.965 kb on - strand at 15.100 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 12,145 + GFF35 0.57 +1.5 12,201 + GFF35 0.60 +0.3 12,342 - GFF35 0.66 +0.3 12,342 - GFF35 0.66 -0.0 12,355 - GFF35 0.67 -0.9 12,359 + GFF35 0.67 +0.4 12,379 + GFF35 0.68 -2.8 12,379 + GFF35 0.68 -0.7 12,379 + GFF35 0.68 +0.4 12,383 + GFF35 0.68 +0.3 12,396 - GFF35 0.69 -0.0 12,425 + GFF35 0.70 +0.5 12,425 + GFF35 0.70 +0.0 12,426 - GFF35 0.70 +0.0 12,426 - GFF35 0.70 +0.4 12,426 - GFF35 0.70 +0.1 12,427 + GFF35 0.70 +0.4 12,427 + GFF35 0.70 -0.5 12,428 - GFF35 0.70 -0.6 12,428 - GFF35 0.70 +0.4 12,634 + GFF35 0.80 +0.3 12,923 + -0.2 13,025 + -0.8 13,026 - +0.2 13,099 - +0.6 13,136 + -0.4 13,144 + +0.7 13,192 + -0.6 13,211 + +0.7 13,246 + +0.2 13,247 - -1.1 13,326 - GFF36 0.10 +0.5 13,424 - GFF36 0.16 -0.6 13,514 + GFF36 0.21 -1.9 13,517 - GFF36 0.21 -0.3 13,517 - GFF36 0.21 -0.1 13,517 - GFF36 0.21 +0.5 13,708 + GFF36 0.32 -0.4 13,709 - GFF36 0.32 +0.4 13,709 - GFF36 0.32 -0.3 13,709 - GFF36 0.32 +0.3 13,743 - GFF36 0.34 +1.1 13,754 - GFF36 0.35 +0.4 13,774 - GFF36 0.36 -0.0 13,927 + GFF36 0.44 +0.7 14,040 + GFF36 0.51 +0.3 14,097 + GFF36 0.54 +0.6 14,098 - GFF36 0.54 +0.2 14,102 + GFF36 0.54 +0.0 14,236 + GFF36 0.62 +0.8 14,242 + GFF36 0.62 +0.6 14,243 - GFF36 0.62 -0.3 14,246 + GFF36 0.63 +0.4 14,334 + GFF36 0.68 +0.4 14,335 - GFF36 0.68 +1.4 14,481 - GFF36 0.76 +0.5 14,481 - GFF36 0.76 -0.6 14,483 + GFF36 0.76 +0.3 14,495 + GFF36 0.77 -0.1 14,586 - GFF36 0.82 +0.2 14,814 - -0.0 14,925 + +0.4 14,964 + +1.1 14,965 - +1.0 15,100 + +4.2
Or see this region's nucleotide sequence